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anti-Mouse (Murine) CD25 antibody for Functional Studies

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
574
460
169
19
10
10
8
5
4
3
3
2
1
1
Host
Rat
607
411
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
125
102
61
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
949
149
141
132
130
124
81
75
47
21
20
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1302515
$ 196.35
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086938485 ABIN370891 Func FACS IP Rat IgG2b Log in to see 3C7 15
0.00086938485 ABIN1302435 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5 6
0.00086938485 ABIN370892 Func FACS IP Rat IgG2b Log in to see 3C7 15
0.00086938485 ABIN1302633 Func IHC (fro) FACS IP APC Rat IgG1 Log in to see PC61-5 8
0.00086938485 ABIN1302926 Func IHC (fro) FACS IP FITC Rat IgG1 Log in to see PC61-5 8
0.00086938485 ABIN1303127 Func IHC (fro) FACS IP PE Rat IgG1 Log in to see PC61-5 8
0.00086938485 ABIN282164 Func FACS Rat IgG Mix lambda Log in to see PC61-5
0.00086938485 ABIN2479486 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
0.00086938485 ABIN601654 Func IHC (fro) FACS IP Rat IgG1 Log in to see
0.00086938485 ABIN3200888 FACS Func IHC (fro) IP PE Rat IgG1 Log in to see PC61-5
0.00086938485 ABIN3199664 FACS Func IHC (fro) IP FITC Rat IgG1 Log in to see PC61-5
0.00086938485 ABIN3199663 FACS Func IHC (fro) IP APC Rat IgG1 Log in to see PC61-5
0.00086938485 ABIN2567369 Func FACS IP IHC Rat IgG1 lambda Log in to see PC61-5
0.00086938485 ABIN2567371 Func FACS IP IHC Rat IgG1 lambda Log in to see PC61-5
0.00086938485 ABIN2567370 Func FACS IP IHC Rat IgG1 lambda Log in to see PC61-5
0.00086938485 ABIN2678213 Func FACS Rat IgG2b kappa Log in to see 3C7 (3C7)
0.00086938485 ABIN3199661 FACS Func IHC (fro) IP Rat IgG1 Log in to see PC61-5
0.00086938485 ABIN4291004 FACS Func PE Rat IgG2b kappa Log in to see RM134L
0.00086938485 ABIN4291005 FACS Func PE Rat IgG2b kappa Log in to see RM134L
0.00086938485 ABIN4291006 FACS Func PE Rat IgG2b kappa Log in to see RM134L

Top referenced anti-CD25 antibody for Functional Studies

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 15 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 397 Antibodies
Functional Studies (Func) 28 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 51 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 84 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(574), (460), (169), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Rat
(607), (411), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (125), (102), (61), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(949), (149), (141), (132), (130), (124), (81), (75), (47), (21), (20), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1302515) Images
Specificity The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen B6.1 CTL cell line
Clone PC61-5
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1302515) Images back to top
Antigen
Alternative Name IL2RA (IL2RA Antibody Abstract)
Background CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1302515) Images back to top
Application Notes Functional Application: In vitro blocking of IL-2 binding to its receptor (CD25) and inhibition of IL-2 stimulated cell growth. In vivo depletion of CD25 positive cells.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens & MHC (Rodent)

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1302515) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD25 Antibody (ABIN1302515)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Tenorio, Olguín, Fernández, Vieyra, Saavedra: "Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 786078, 2009

Wei, Chuang, Li, Wei, Sun, Moritoki, Gershwin, Lian, Tian: "CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 10, pp. 7221-9, 2008

Van, Darwiche, Raymond, Lesage, Bouguermouh, Rubio, Sarfati: "Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 8, pp. 5204-8, 2008

Kotner, Tarleton: "Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice." in: Infection and immunity, Vol. 75, Issue 2, pp. 861-9, 2007

Kish, Gorbachev, Fairchild: "CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses." in: Journal of leukocyte biology, Vol. 78, Issue 3, pp. 725-35, 2005

Yu, Feng, Shih, Lai: "Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection." in: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4556-66, 2002

Jones, Cose, Coles, Winterhalter, Brooks, Heath, Carbone: "Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection." in: Journal of virology, Vol. 74, Issue 5, pp. 2414-9, 2000

Aiba, Hirayama, Ogawa: "Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells." in: Blood, Vol. 89, Issue 11, pp. 4005-12, 1997