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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||16 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Flow Cytometry (FACS)||280 Antibodies|
|Functional Studies (Func)||25 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||90 Antibodies|
|In vitro Assay (in vitro)||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||17 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
|15 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN370891) Images
|Specificity||This antibody is specific to Low affinity alpha chain IL-2 Receptor (IL-2Ra), Mr 55 kDa. The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. (Ref.2,11,12) Clone 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. (Ref.2,13)|
|Characteristics||Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen|
|Endotoxin Level||Low Endotoxin|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN370891) Images back to top
|Alternative Name||CD25 / IL2RA (IL2RA Antibody Abstract)|
|Background||The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. (Ref.14) CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. (Ref.2,3,15) Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. (Ref.4) CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. (Ref.5) It is expressed at a higher level on CD4+CD8+ thymocytes. (Ref.5,6) It is also expressed on cultured epidermal Langerhans cells. (Ref.7) The biochemical features of murine CD25 have been characterized in detail. (Ref.8-10)Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN370891) Images back to top
Flow Cytometry: Identification and enumeration of CD25+ (Ref.1-4)Immunoprecipitation. (Ref.1,2,8,10)Functional Assay: Inhibition of IL-2 or mitogen-induced T cell proliferation. (Ref.13)In vitro blocking of IL-2 binding. (Ref.2,12)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN370891) Images back to top
|Buffer||PBS containing no preservatives.|
Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD25 Antibody (ABIN370891)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Chen, Ma, Young et al.: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995 (PubMed).
Rolink, Grawunder, Winkler et al.: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, 1995 (PubMed).
Malek, Ortega, Jakway et al.: "The murine IL 2 receptor. II. Monoclonal anti-IL 2 receptor antibodies as specific inhibitors of T cell function in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1976-82, 1984 (PubMed).
Malek, Robb, Shevach: "Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 18, pp. 5694-8, 1983 (PubMed).
Ortega, Robb, Shevach et al.: "The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1970-5, 1984 (PubMed).
Leonard, Depper, Crabtree et al.: "Molecular cloning and expression of cDNAs for the human interleukin-2 receptor." in: Nature, Vol. 311, Issue 5987, pp. 626-31, 1984 (PubMed).
Habu, Okumura, Diamantstein et al.: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, 1985 (PubMed).
Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, 1985 (PubMed).
Andrew, Churilla, Malek et al.: "Activation of virus specific CTL clones: antigen-dependent regulation of interleukin 2 receptor expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 2, pp. 920-5, 1985 (PubMed).
Saragovi, Malek: "Direct identification of the murine IL-2 receptor p55-p75 heterodimer in the absence of IL-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 476-82, 1988 (PubMed).
Malek, Ashwell, Germain et al.: "The murine interleukin-2 receptor: biochemical structure and regulation of expression." in: Immunological reviews, Vol. 92, pp. 81-101, 1986 (PubMed).
Steiner, Tschachler, Tani et al.: "Interleukin 2 receptors on cultured murine epidermal Langerhans cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 1, pp. 155-9, 1986 (PubMed).
Malek, Korty: "The murine interleukin 2 receptor. IV. Biochemical characterization." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 11, pp. 4092-8, 1986 (PubMed).
Moreau, Nabholz, Diamantstein et al.: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987 (PubMed).
Lorenzo, Jaulin, Vita et al.: "Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 2970-7, 1991 (PubMed).