You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) CD25 antibody for Functional Studies

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
443
331
109
19
10
10
8
5
4
3
3
2
1
Host
Rat
482
302
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
114
113
91
61
28
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
675
129
117
95
92
70
59
47
44
21
17
15
14
11
10
9
6
6
5
3
2
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN370891
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008738671 ABIN1302515 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5 8
0.0008738671 ABIN370892 Func FACS IP Rat IgG2b Log in to see 3C7 15
0.0008738671 ABIN1302435 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5 6
0.0008738671 ABIN1302633 Func IHC (fro) FACS IP APC Rat IgG1 Log in to see PC61-5 8
0.0008738671 ABIN1302926 Func IHC (fro) FACS IP FITC Rat IgG1 Log in to see PC61-5 8
0.0008738671 ABIN1303127 Func IHC (fro) FACS IP PE Rat IgG1 Log in to see PC61-5 8
0.0008738671 ABIN282164 Func FACS Rat IgG Mix lambda Log in to see PC61-5
0.0008738671 ABIN2479486 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3 4
0.0008738671 ABIN601654 Func IHC (fro) FACS IP Rat IgG1 Log in to see
0.0008738671 ABIN3200888 FACS Func IHC (fro) IP PE Rat IgG1 Log in to see PC61-5
0.0008738671 ABIN3199663 FACS Func IHC (fro) IP APC Rat IgG1 Log in to see PC61-5
0.0008738671 ABIN3199664 FACS Func IHC (fro) IP FITC Rat IgG1 Log in to see PC61-5
0.0008738671 ABIN3199661 FACS Func IHC (fro) IP Rat IgG1 Log in to see PC61-5
0.0008738671 ABIN1819631 Func IHC (fro) FACS IP Rat IgG1 Log in to see PC61-5-3
0.0008738671 ABIN2567371 Func FACS IP IHC Rat IgG1 lambda Log in to see PC61-5
0.0008738671 ABIN2567369 Func FACS IP IHC Rat IgG1 lambda Log in to see PC61-5
0.0008738671 ABIN2567370 Func FACS IP IHC Rat IgG1 lambda Log in to see PC61-5
0.0008738671 ABIN2678213 Func FACS Rat IgG2b kappa Log in to see 3C7 (3C7)
0.0008738671 ABIN3199662 FACS Func IHC (fro) IP Rat IgG1 Log in to see PC61-5
0.0008738671 ABIN3038746 Func FACS IP IHC PE Rat IgG1 Log in to see

Top referenced anti-CD25 antibody for Functional Studies

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 14 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 280 Antibodies
Functional Studies (Func) 25 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 90 Antibodies
In vitro Assay (in vitro) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 17 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(443), (331), (109), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1)
Host Rat
(482), (302), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(114), (113), (91), (61), (28), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(675), (129), (117), (95), (92), (70), (59), (47), (44), (21), (17), (15), (14), (11), (10), (9), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 15 references available
Supplier Log in to see

Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN370891) Images
Specificity This antibody is specific to Low affinity alpha chain IL-2 Receptor (IL-2Ra), Mr 55 kDa. The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. (Ref.2,11,12) Clone 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. (Ref.2,13)
Characteristics Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen
Endotoxin Level Low Endotoxin
Clone 3C7
Isotype IgG2b

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN370891) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. (Ref.14) CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. (Ref.2,3,15) Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. (Ref.4) CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. (Ref.5) It is expressed at a higher level on CD4+CD8+ thymocytes. (Ref.5,6) It is also expressed on cultured epidermal Langerhans cells. (Ref.7) The biochemical features of murine CD25 have been characterized in detail. (Ref.8-10)Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 16184
UniProt P01590
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN370891) Images back to top
Application Notes Flow Cytometry: Identification and enumeration of CD25+ (Ref.1-4)Immunoprecipitation. (Ref.1,2,8,10)Functional Assay: Inhibition of IL-2 or mitogen-induced T cell proliferation. (Ref.13)In vitro blocking of IL-2 binding. (Ref.2,12)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN370891) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing no preservatives.
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD25 Antibody (ABIN370891)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, 1995

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, 1995

Lorenzo, Jaulin, Vita, Froussard, Ferrara, Jankovic, Theze: "Structure-function study of the p55 subunit of murine IL-2 receptor by epitope mapping." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 2970-7, 1991

Saragovi, Malek: "Direct identification of the murine IL-2 receptor p55-p75 heterodimer in the absence of IL-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 476-82, 1988

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987

Steiner, Tschachler, Tani, Malek, Shevach, Holter, Knapp, Wolff, Stingl: "Interleukin 2 receptors on cultured murine epidermal Langerhans cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 1, pp. 155-9, 1986

Malek, Korty: "The murine interleukin 2 receptor. IV. Biochemical characterization." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 11, pp. 4092-8, 1986

Malek, Ashwell, Germain, Shevach, Miller: "The murine interleukin-2 receptor: biochemical structure and regulation of expression." in: Immunological reviews, Vol. 92, pp. 81-101, 1986

Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, 1985

Andrew, Churilla, Malek, Braciale, Braciale: "Activation of virus specific CTL clones: antigen-dependent regulation of interleukin 2 receptor expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 2, pp. 920-5, 1985

Habu, Okumura, Diamantstein, Shevach: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, 1985

Ortega, Robb, Shevach, Malek: "The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1970-5, 1984

Malek, Ortega, Jakway, Chan, Shevach: "The murine IL 2 receptor. II. Monoclonal anti-IL 2 receptor antibodies as specific inhibitors of T cell function in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1976-82, 1984

Leonard, Depper, Crabtree, Rudikoff, Pumphrey, Robb, Krönke, Svetlik, Peffer, Waldmann: "Molecular cloning and expression of cDNAs for the human interleukin-2 receptor." in: Nature, Vol. 311, Issue 5987, pp. 626-31, 1984

Malek, Robb, Shevach: "Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 18, pp. 5694-8, 1983