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anti-Mouse (Murine) CD25 antibody for Intracellular Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
443
331
109
19
10
10
8
5
4
3
3
2
1
Host
Rat
482
302
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to PE-Cy7
114
113
91
61
27
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
676
130
117
95
92
70
59
47
45
20
17
15
14
11
10
9
6
6
5
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2659473
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008704084 ABIN2660661 ICFC APC-Cy7 Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN2661619 ICFC FITC Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN2661621 ICFC FITC Rat IgG2b kappa Log in to see 3C7 7
0.0008704084 ABIN2660326 ICFC PerCP-Cy5.5 Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN2660059 ICFC PerCP-Cy5.5 Rat IgG2b kappa Log in to see 3C7 6
0.0008704084 ABIN2659844 ICFC PerCP Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN2663968 ICFC PE Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN2659474 ICFC PE-Cy7 Rat IgG1 lambda Log in to see PC61 14
0.0008704084 ABIN2660662 ICFC APC-Cy7 Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN2661620 ICFC FITC Rat IgG2b kappa Log in to see 3C7 7
0.0008704084 ABIN2661618 ICFC FITC Rat IgG1 lambda Log in to see PC61 13
0.0008704084 ABIN2660327 ICFC PerCP-Cy5.5 Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN2660060 ICFC PerCP-Cy5.5 Rat IgG2b kappa Log in to see 3C7 6
0.0008704084 ABIN2659845 ICFC PerCP Rat IgG1 lambda Log in to see PC61 14
0.0008704084 ABIN2663969 ICFC PE Rat IgG1 lambda Log in to see PC61 12
0.0008704084 ABIN1176950 BR FACS ICFC Rat IgG2b kappa Log in to see 3C7

Top referenced anti-CD25 antibody for Intracellular Flow Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 14 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 280 Antibodies
Functional Studies (Func) 25 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 90 Antibodies
In vitro Assay (in vitro) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 17 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(443), (331), (109), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1)
Host Rat
(482), (302), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to PE-Cy7
(114), (113), (91), (61), (27), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(676), (130), (117), (95), (92), (70), (59), (47), (45), (20), (17), (15), (14), (11), (10), (9), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2659473) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Clone PC61
Isotype IgG1 lambda

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2659473) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2659473) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2659473) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 Antibody (ABIN2659473)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

León-Ponte, Ahern, OConnell: "Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor." in: Blood, Vol. 109, Issue 8, pp. 3139-46, 2007

Lei, Scott: "Induction of tolerance to factor VIII inhibitors by gene therapy with immunodominant A2 and C2 domains presented by B cells as Ig fusion proteins." in: Blood, Vol. 105, Issue 12, pp. 4865-70, 2005

Pasare, Medzhitov: "Toll-dependent control mechanisms of CD4 T cell activation." in: Immunity, Vol. 21, Issue 5, pp. 733-41, 2004

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000

Onizuka, Tawara, Shimizu, Sakaguchi, Fujita, Nakayama: "Tumor rejection by in vivo administration of anti-CD25 (interleukin-2 receptor alpha) monoclonal antibody." in: Cancer research, Vol. 59, Issue 13, pp. 3128-33, 1999

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987

Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985

Lowenthal, Zubler, Nabholz, MacDonald: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985

Ceredig, Lowenthal, Nabholz, MacDonald: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, 1985

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2659473) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (PE-Cy7) (ABIN2659473) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PE-Cy7)