We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||16 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||1 Antibodies|
|ELISA (Detection)||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Flow Cytometry (FACS)||280 Antibodies|
|Functional Studies (Func)||25 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||90 Antibodies|
|In vitro Assay (in vitro)||4 Antibodies|
|Intracellular Flow Cytometry (ICFC)||17 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD25 antibody is conjugated to PE Alternatives|
Intracellular Flow Cytometry (ICFC)
|12 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2663968) Images
|Purification||The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2663968) Images back to top
|Alternative Name||CD25 (IL2RA Antibody Abstract)|
|Background||CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2663968) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2663968) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CD25 Antibody (ABIN2663968)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
León-Ponte, Ahern, OConnell: "Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor." in: Blood, Vol. 109, Issue 8, pp. 3139-46, 2007 (PubMed).
Lei, Scott: "Induction of tolerance to factor VIII inhibitors by gene therapy with immunodominant A2 and C2 domains presented by B cells as Ig fusion proteins." in: Blood, Vol. 105, Issue 12, pp. 4865-70, 2005 (PubMed).
Pasare, Medzhitov: "Toll-dependent control mechanisms of CD4 T cell activation." in: Immunity, Vol. 21, Issue 5, pp. 733-41, 2004 (PubMed).
Takahashi, Tagami, Yamazaki et al.: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000 (PubMed).
Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000 (PubMed).
Onizuka, Tawara, Shimizu et al.: "Tumor rejection by in vivo administration of anti-CD25 (interleukin-2 receptor alpha) monoclonal antibody." in: Cancer research, Vol. 59, Issue 13, pp. 3128-33, 1999 (PubMed).
Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).
Lowenthal, Corthésy, Tougne et al.: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985 (PubMed).
Lowenthal, Zubler, Nabholz et al.: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985 (PubMed).
Ceredig, Lowenthal, Nabholz et al.: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, 1985 (PubMed).
Moreau, Nabholz, Diamantstein et al.: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987 (PubMed).
Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991 (PubMed).
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2663968) back to top