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anti-Mouse (Murine) CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Epitope
AA 34-139
22
20
19
18
15
14
14
7
7
6
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
563
435
150
19
10
9
5
4
3
3
2
1
1
Host
Mouse
569
383
135
7
5
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
132
121
95
57
27
27
25
23
21
19
17
13
12
10
10
10
10
10
10
10
8
8
8
6
6
6
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
891
151
143
131
112
81
80
75
45
21
20
19
17
12
10
9
7
6
6
5
4
3
3
2
2
1
1
1
Supplier
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Catalog No. ABIN1724861
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN2705770 WB Rabbit Center Log in to see Polyclonal
0.124960825 ABIN2490712 IF ELISA WB Rabbit IgG Ser268 Log in to see Polyclonal
0.124960825 ABIN1873210 WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN2490713 IF ELISA WB Rabbit IgG pSer268 Log in to see Polyclonal
0.124960825 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal
0.124960825 ABIN2426093 ELISA WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN1845343 ICC IF IHC ELISA WB Mouse IgG1 AA 34-139 Log in to see
0.124960825 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12
0.0012207722 ABIN2658044 WB Alexa Fluor 700 Rat IgG1 lambda Log in to see PC61 13
0.0012207722 ABIN1584197 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012207722 ABIN4880789 ELISA ICC IHC WB Mouse IgG1 AA 34-139 Log in to see 1B5D12
0.0012207722 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal
0.0012207722 ABIN670380 IHC (p) WB HRP Rabbit IgG AA 258-268 Log in to see Polyclonal
0.0012207722 ABIN670373 IHC (p) WB Biotin Rabbit IgG AA 258-268 Log in to see Polyclonal
0.0012207722 ABIN2624038 ELISA WB Biotin Rabbit IgG AA 22-235 Log in to see Polyclonal
0.0012207722 ABIN2624039 WB FITC Rabbit IgG AA 22-235 Log in to see Polyclonal
0.0012207722 ABIN4291121 FACS WB DyLight 405 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.0012207722 ABIN4291107 FACS WB Alexa Fluor 647 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.0012207722 ABIN4291108 FACS WB Alexa Fluor 700 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
0.0012207722 ABIN4291113 FACS WB DyLight 680 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab

Top referenced anti-CD25 antibody for Western Blotting

  • Human Monoclonal CD25 Primary Antibody for ICC, IHC - ABIN1724861 (2 Publications): Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients. in Annals of hematology 2012 (PubMed)

  • Mouse (Murine) Monoclonal CD25 Primary Antibody for WB - ABIN2658044 (13 Publications): Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. in Journal of immunology (Baltimore, Md. : 1950) 1985 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
ELISA 48 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 114 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 40 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 34-139
(22), (20), (19), (18), (15), (14), (14), (7), (7), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Monkey, Rat (Rattus)
(563), (435), (150), (19), (10), (9), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(569), (383), (135), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(132), (121), (95), (57), (27), (27), (25), (23), (21), (19), (17), (13), (12), (10), (10), (10), (10), (10), (10), (10), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(891), (151), (143), (131), (112), (81), (80), (75), (45), (21), (20), (19), (17), (12), (10), (9), (7), (6), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images
Purification Purified
Immunogen Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Clone 1B5D12
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Antigen
Alternative Name IL2RA (IL2RA Antibody Abstract)
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight 30.8 kDa
Gene ID 3559
HGNC 3559
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1724861) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD25 Antibody (ABIN1724861)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) back to top
Supplier Images
ELISA image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
Immunocytochemistry (ICC) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5)