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anti-Mouse (Murine) CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Epitope
AA 34-139
13
7
7
5
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
472
425
126
19
10
10
8
4
4
3
3
2
1
Host
Mouse
527
397
91
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
122
120
97
61
30
22
20
19
17
14
12
12
11
10
7
7
7
7
7
7
7
6
6
6
6
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
817
130
116
112
106
104
61
59
45
21
17
14
14
11
10
9
6
6
5
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1724861
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12471054 ABIN2705770 WB Rabbit Center Log in to see Polyclonal
0.12471054 ABIN1873210 WB Rabbit IgG Log in to see Polyclonal
0.12471054 ABIN2490712 IF ELISA WB Rabbit IgG Ser268 Log in to see Polyclonal
0.12471054 ABIN2490713 IF ELISA WB Rabbit IgG pSer268 Log in to see Polyclonal
0.12471054 ABIN1859413 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-235 Log in to see Polyclonal
0.12471054 ABIN2405718 WB Rabbit IgG Log in to see Polyclonal
0.12471054 ABIN1845343 ICC IF IHC ELISA WB Mouse IgG1 AA 34-139 Log in to see
0.12471054 ABIN2426093 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011780028 ABIN2658044 WB Alexa Fluor 700 Rat IgG1 lambda Log in to see PC61 13
0.0011780028 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal
0.0011780028 ABIN1584197 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011780028 ABIN670373 IHC (p) WB Biotin Rabbit IgG AA 258-268 Log in to see Polyclonal
0.0011780028 ABIN670380 IHC (p) WB HRP Rabbit IgG AA 258-268 Log in to see Polyclonal
0.0011780028 ABIN2624038 ELISA WB Biotin Rabbit IgG AA 22-235 Log in to see Polyclonal
0.0011780028 ABIN2624039 WB FITC Rabbit IgG AA 22-235 Log in to see Polyclonal
0.0011780028 ABIN2442401 WB Rabbit N-Term Log in to see Polyclonal
0.0011780028 ABIN2742054 FACS IHC WB Rat IgG1, lambda Log in to see PC61
0.0011780028 ABIN2971873 WB Rabbit Center Log in to see Polyclonal
0.0011780028 ABIN2974449 WB Rabbit IgG Log in to see Polyclonal
0.0011780028 ABIN1884989 IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-CD25 antibody for Western Blotting

  • Human Monoclonal CD25 Primary Antibody for ICC, IHC - ABIN1724861 (2 Publications): Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients. in Annals of hematology 2012 (PubMed)

  • Mouse (Murine) Monoclonal CD25 Primary Antibody for WB - ABIN2658044 (13 Publications): Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. in Journal of immunology (Baltimore, Md. : 1950) 1985 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies 4 Antibodies
ELISA 50 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 95 Antibodies
Functional Studies (Func) 7 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 46 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 20 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 34-139
(13), (7), (7), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Monkey, Rat (Rattus)
(472), (425), (126), (19), (10), (10), (8), (4), (4), (3), (3), (2), (1)
Host Mouse
(527), (397), (91), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(122), (120), (97), (61), (30), (22), (20), (19), (17), (14), (12), (12), (11), (10), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(817), (130), (116), (112), (106), (104), (61), (59), (45), (21), (17), (14), (14), (11), (10), (9), (6), (6), (5), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images
Purification Purified
Immunogen Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Clone 1B5D12
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Antigen
Alternative Name IL2RA (IL2RA Antibody Abstract)
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight 30.8 kDa
Gene ID 3559
HGNC 3559
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1724861) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1724861) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD25 Antibody (ABIN1724861)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1724861) back to top
Supplier Images
ELISA image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
Immunocytochemistry (ICC) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4)
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (AA 34-139) (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5)