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anti-Non-Human Primate CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human, Non-Human Primate
576
462
170
19
10
10
7
5
4
3
3
2
1
1
Host
Mouse
608
411
135
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to APC
131
125
102
60
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
947
149
142
132
130
125
84
74
48
21
20
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN2678204
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2678202 FACS PE Mouse IgG2b Log in to see CD25-4E3
0.30226976 ABIN2678212 FACS PE-eFluor® 610 Mouse IgG2b kappa Log in to see CD25-4E3
0.30226976 ABIN2678210 FACS EFluor 450 Mouse IgG2b kappa Log in to see CD25-4E3
0.30226976 ABIN2678208 FACS PerCP-eFluor® 710 Mouse IgG2b Log in to see CD25-4E3
0.30226976 ABIN2678206 FACS PE-Cy7 Mouse IgG2b kappa Log in to see CD25-4E3

Similar anti-CD25 Antibodies

Application / Reactivity Human Non-Human Primate
Western Blotting (WB) 124 Antibodies
Neutralization (Neut) 17 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunofluorescence (IF) 68 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Functional Studies (Func) 13 Antibodies
Flow Cytometry (FACS) 420 Antibodies 6 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISA (Capture) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human, Non-Human Primate
(576), (462), (170), (19), (10), (10), (7), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(608), (411), (135), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to APC
(131), (125), (102), (60), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(947), (149), (142), (132), (130), (125), (84), (74), (48), (21), (20), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes.

This monoclonal antibody is cross-reactive to rhesus monkey.
Characteristics This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes. This monoclonal antibody is cross-reactive to rhesus monkey.
Synonyms: Synonyms: Interleukin-2 Receptor alpha, IL-2Ra
Clone CD25-4E3
Isotype IgG2b kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Applications reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis.

Applications tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of nrmal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (APC) (ABIN2678204) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (APC)