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anti-Rat (Rattus) CD25 antibody for Functional Studies

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Rat (Rattus)
443
332
108
19
10
10
8
5
4
3
3
2
1
Host
Mouse
481
303
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
114
113
91
61
28
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
675
129
117
95
92
69
59
46
44
21
17
15
14
11
10
8
6
6
5
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN120426
$ 588.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008821086 ABIN120431 EIA Func FACS IHC (fro) Mouse IgG1 Log in to see NDS 61
0.0008821086 ABIN2479468 Func IHC (fro) FACS ELISA Mouse IgG1 Log in to see NDS 61 4
0.0008821086 ABIN217773 Func FACS IHC (fro) IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39
0.0008821086 ABIN2479475 Func IHC (fro) FACS IHC (p) IP ELISA Mouse IgG1 Log in to see OX-39 4
0.0008821086 ABIN210184 Func FACS IHC (fro) ELISA Mouse IgG1 Log in to see NDS 61
0.0008821086 ABIN2678227 Func FACS Mouse IgG1 Log in to see OX39

Top referenced anti-CD25 antibody for Functional Studies

Similar anti-CD25 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 1 Antibodies
ELISA 32 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 77 Antibodies
Functional Studies (Func) 7 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 28 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 20 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Rat (Rattus)
(443), (332), (108), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1)
Host Mouse
(481), (303), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(114), (113), (91), (61), (28), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(675), (129), (117), (95), (92), (69), (59), (46), (44), (21), (17), (15), (14), (11), (10), (8), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN120426) Images
Specificity This antibody recognizes the alpha chain of CD25, otherwise know as IL-2 receptor alpha. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
Purification Affinity chromatography on Protein G
Endotoxin Level Low Endotoxin
Immunogen Stimulated Rat T cells. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/1 mouse myeloma cell line.
Clone OX-39
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN120426) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background CD25 is a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 25704
NCBI Accession NP_037295
UniProt P26897
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN120426) Images back to top
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Functional Assays. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN120426) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-CD25 Antibody (ABIN120426)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Charteris, Lightman: "In vivo lymphokine production in experimental autoimmune uveoretinitis." in: Immunology, Vol. 78, Issue 3, pp. 387-92, 1993

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988

Hayosh, Swanborg: "Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 11, pp. 3771-5, 1987

Tellides, Dallman, Kupiec-Weglinski, Diamantstein, Morris: "Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy." in: Transplantation proceedings, Vol. 19, Issue 5, pp. 4231-3, 1987

Signore, Parman, Pozzilli, Andreani, Beverley: "Detection of activated lymphocytes in endocrine pancreas of BB/W rats by injection of 123I-interleukin-2: an early sign of type 1 diabetes." in: Lancet, Vol. 2, Issue 8558, pp. 537-40, 1987