You are viewing an incomplete version of our website. Please click to reload the website as full version.

Mouse (Murine) CSF2 ELISA Kit

Recommended CSF2 Product (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kits
  • CSF2
  • GM-CSF
  • GMCSF
  • CSF
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • granulocyte-macrophage colony-stimulating-factor
  • colony stimulating factor 2 (granulocyte-macrophage)
  • granulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • GM-CSF
  • CSF2
  • Csf2
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
21
20
17
8
4
4
3
3
3
2
2
1
Methode Type
Sandwich ELISA
Detection Range
6-2000 pg/mL
Minimum Detection Limit
6 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN625125
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.876543 ABIN415372 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.37-1000 pg/mL 1.37 pg/mL Log in to see 1
12.876543 ABIN411277 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 15.6-1000 pg/mL 15.6 pg/mL Log in to see 2
12.876543 ABIN366335 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.876543 ABIN1115250 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN627863 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 25-500 pg/mL 25 pg/mL Log in to see
1 ABIN1570085 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2683928 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2683929 Sandwich ELISA Cell Culture Supernatant, Cell Lysate 15.6-500 pg/mL 15.6 pg/mL Log in to see
1 ABIN2683930 Sandwich ELISA Cell Culture Supernatant, Serum 3.9-250 pg/mL 3.9 pg/mL Log in to see
1 ABIN2683931 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Serum 50-3200 pg/mL 50 pg/mL Log in to see
1 ABIN4947464 Sandwich ELISA Log in to see
1 ABIN2543220 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
1 ABIN2116000 0.62-10 ng/mL 0.62 ng/mL Log in to see
1 ABIN2111989 Sandwich ELISA 50-3200 pg/mL 50 pg/mL Log in to see
1 ABIN455255 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2869713 Log in to see
1 ABIN2948013 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2116002 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced CSF2 ELISA Kit for ELISA

Similar CSF2 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 19 ELISA Kits

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(21), (20), (17), (8), (4), (4), (3), (3), (3), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 6-2000 pg/mL
Minimum Detection Limit 6 pg/mL
Application ELISA
Pubmed 6 references available
Supplier Log in to see

Product Details CSF2 ELISA Kit

Target details Application Details Handling References for CSF2 Kit (ABIN625125) Images
Purpose Mouse GM-CSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Serum, Plasma, Cell Culture Supernatant
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity < 6 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details CSF2 ELISA Kit Application Details Handling References for CSF2 Kit (ABIN625125) Images back to top
Antigen
Alternative Name GM-CSF/ CSF2 (CSF2 ELISA Kit Abstract)
Background The Mouse GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse GM-CSF in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for mouse GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 12983
UniProt P26955
Research Area Immunology, Innate Immunity, Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details CSF2 ELISA Kit Target details Handling References for CSF2 Kit (ABIN625125) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 40 µL GM-CSF standard from the vial of Item C, into a tube with 960 µL Assay Diluent A or 1x Assay Diluent B to prepare a 2000 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200myl 200 µL 200 µL 200 µL 200 µL 40 µL standard + 960 µL 2000 666.7 222.2 74.07 24.69 8.23 2.74 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 60 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a 200-fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Mouse GM-CSF concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Mouse GM-CSF concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of GM-CSF is typically less than 6 pg/mL.
Recovery: Recovery was determined by spiking various levels of mouse GM-CSF into mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 95.48 84-103 Plasma 100.32 89-107 Cell culture media 102.53 88-108
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 95 97 96 Range ( %) 82-102 84-103 83-102 1:4 Average % of Expected 98 95 102 Range ( %) 85-104 83-103 88-108
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details CSF2 ELISA Kit Target details Application Details References for CSF2 Kit (ABIN625125) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for CSF2 Kit (ABIN625125)

Product Details CSF2 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Zhang, Ren, Tang, Wang, Liu, Zhang, Li, Liu, Zhao, He: "Vascular normalization induced by sinomenine hydrochloride results in suppressed mammary tumor growth and metastasis." in: Scientific reports, Vol. 5, pp. 8888, 2015

Mishra, Patel, Bansal, Kumar: "Semiquinone glucoside derivative provides protection against ?-radiation by modulation of immune response in murine model." in: Environmental toxicology, 2014

Ye, Chen, Wu, Huang, Li, Wang, Liu, Wang, Yang, Zhang, Lv, Xia: "GM-CSF contributes to aortic aneurysms resulting from SMAD3 deficiency." in: The Journal of clinical investigation, Vol. 123, Issue 5, pp. 2317-31, 2013

Ye, Chen, Wu, Huang, Li, Wang, Liu, Wang, Yang, Zhang, Lv, Xia: "GM-CSF contributes to aortic aneurysms resulting from SMAD3 deficiency." in: The Journal of clinical investigation, Vol. 123, Issue 5, pp. 2317-31, 2013

Ye, Chen, Wu, Huang, Li, Wang, Liu, Wang, Yang, Zhang, Lv, Xia: "GM-CSF contributes to aortic aneurysms resulting from SMAD3 deficiency." in: The Journal of clinical investigation, Vol. 123, Issue 5, pp. 2317-31, 2013

Ye, Chen, Wu, Huang, Li, Wang, Liu, Wang, Yang, Zhang, Lv, Xia: "GM-CSF contributes to aortic aneurysms resulting from SMAD3 deficiency." in: The Journal of clinical investigation, Vol. 123, Issue 5, pp. 2317-31, 2013

Images

Product Details CSF2 ELISA Kit Target details Application Details Handling References for CSF2 Kit (ABIN625125) back to top
Supplier Images
ELISA image for CSF2 ELISA Kit (Colony Stimulating Factor 2 (Granulocyte-Macrophage)) (ABIN625125) Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kit