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Rat (Rattus) CSF2 ELISA Kit

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Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kits
  • CSF
  • CSF2
  • Csfgm
  • Gm-csf
  • Gm-CSf
  • GM-CSF
  • GMCSF
  • Gmcsf
  • MGI-IGM
Epitope
AA 1-127
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
48
45
33
11
9
7
5
5
4
4
3
2
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921059
$ 350.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.29526275 ABIN416077 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.29526275 ABIN1115251 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.29526275 ABIN2708644 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.29526275 ABIN2762217 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 0.02-1.0 ng/mL 0.02-1.0 ng/mL Log in to see
0.0012266339 ABIN368073 Sandwich ELISA Serum, Plasma, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Log in to see
0.0012266339 ABIN3032868 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-3000 pg/mL 4.1 pg/mL Log in to see
0.0012266339 ABIN625194 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 10-3000 pg/mL 10 pg/mL Log in to see
0.0012266339 ABIN3198538 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012266339 ABIN627048 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012266339 ABIN1570086 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012266339 ABIN2683932 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
0.0012266339 ABIN627084 Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.0012266339 ABIN627109 Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.0012266339 ABIN1112618 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012266339 ABIN510106 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012266339 ABIN955824 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012266339 ABIN2748815 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012266339 ABIN2042486 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012266339 ABIN2113569 Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant Log in to see
0.0012266339 ABIN2683933 Sandwich ELISA Cell Culture Supernatant, Serum 7.8-500 pg/mL 7.8 pg/mL Log in to see

Top referenced CSF2 ELISA Kit for ELISA

Similar CSF2 ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 31 ELISA Kits

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kits
Epitope AA 1-127
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(48), (45), (33), (11), (9), (7), (5), (5), (4), (4), (3), (2)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CSF2 ELISA Kit

Target details Application Details Handling References for CSF2 Kit (ABIN921059) Images
Purpose For quantitative detection of rat GM-CSF in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant rat GM-CSF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat GM-CSF antibod - 1
  • Lyophilized recombinant rat GM-CSF standar - 10ng/tubex2
  • Biotinylated anti- rat GM-CSF antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A1-K127

Target details

Product Details CSF2 ELISA Kit Application Details Handling References for CSF2 Kit (ABIN921059) Images back to top
Antigen
Alternative Name CSF2 (CSF2 ELISA Kit Abstract)
Background Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69 % nucleotide homology and 54 % amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
Gene ID 116630
UniProt P48750
Pathways JAK-STAT Signaling

Application Details

Product Details CSF2 ELISA Kit Target details Handling References for CSF2 Kit (ABIN921059) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat GM-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GM-CSF has been precoated onto 96-well plates. Standards(E.coli, A1-K127) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat GM-CSF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL rat GM-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat GM-CSF standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 110, Standard deviation: 4.29, CV(%): 3.9
  • Sample 2: n=16, Mean(pg/ml): 379, Standard deviation: 16.3, CV(%): 4.3
  • Sample 3: n=16, Mean(pg/ml): 646, Standard deviation: 29.07, CV(%): 4.5,
  • Sample 1: n=24, Mean(pg/ml): 125, Standard deviation: 8.5, CV(%): 6.8
  • Sample 2: n=24, Mean(pg/ml): 458, Standard deviation: 31.6, CV(%): 6.9
  • Sample 3: n=24, Mean(pg/ml): 637, Standard deviation: 45.2, CV(%): 7.1
Restrictions For Research Use only

Handling

Product Details CSF2 ELISA Kit Target details Application Details References for CSF2 Kit (ABIN921059) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CSF2 Kit (ABIN921059)

Product Details CSF2 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Grabstein, Urdal, Tushinski, Mochizuki, Price, Cantrell, Gillis, Conlon: "Induction of macrophage tumoricidal activity by granulocyte-macrophage colony-stimulating factor." in: Science (New York, N.Y.), Vol. 232, Issue 4749, pp. 506-8, 1986

Huebner, Isobe, Croce, Golde, Kaufman, Gasson: "The human gene encoding GM-CSF is at 5q21-q32, the chromosome region deleted in the 5q- anomaly." in: Science (New York, N.Y.), Vol. 230, Issue 4731, pp. 1282-5, 1986

Images

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Supplier Images
ELISA image for CSF2 ELISA Kit (Colony Stimulating Factor 2 (Granulocyte-Macrophage)) (ABIN921059) Rat GM-CSF PicoKine ELISA Kit standard curve