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anti-Human FYN antibody for Flow Cytometry

Recommended FYN Antibody (supplied by: Log in to see )

Antigen
FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
  • AI448320
  • AW552119
  • c-fyn
  • cfyn-a
  • fyn
  • im:6908851
  • p59-FYN
  • PKC
  • si:dkey-119m7.5
  • SLK
  • SYN
  • zgc:86720
  • zgc:109996
Reactivity
Human
220
123
39
7
7
7
2
1
1
1
1
Host
Mouse
131
97
16
Clonality
Monoclonal
Conjugate
This FYN antibody is un-conjugated
9
9
8
8
7
7
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
224
113
64
39
38
27
26
18
6
4
2
2
2
2
1
Supplier
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Catalog No. ABIN969159
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3116459 ABIN652383 FACS IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal
0.3116459 ABIN2439365 FACS WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.3116459 ABIN2437181 FACS IHC ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.3116459 ABIN1846351 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010621916 ABIN392098 FACS IHC (p) WB Rabbit Ig AA 13-43, N-Term Log in to see Polyclonal
0.0010621916 ABIN966166 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2A10 2
0.0010621916 ABIN1959883 ICC FACS IF IHC (p) ELISA WB Mouse IgG1 Log in to see 2A10
0.0010621916 ABIN1723376 ICC FACS IHC (p) IP WB Mouse IgG Log in to see FYN-01
0.0010621916 ABIN1812803 FACS IHC (p) WB Rabbit AA 1-30 Log in to see Polyclonal
0.0010621916 ABIN1841323 FACS IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0010621916 ABIN865401 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2A10
0.0010621916 ABIN2573235 FACS Mouse IgG1, kappa Log in to see 1302CT390-118-237
0.0010621916 ABIN2270433 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B328
0.0010621916 ABIN3031009 FACS IHC ELISA WB Rabbit Ig Fraction AA 13-43 Log in to see Polyclonal
0.0010621916 ABIN3031010 FACS IHC ELISA WB Rabbit Ig Fraction AA 1-30 Log in to see Polyclonal
0.0010621916 ABIN4312913 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 2A10
0.0010621916 ABIN3031012 FACS IHC ELISA WB Mouse IgG1, kappa Log in to see 1302CT390-118-237
0.0010621916 ABIN2793188 FACS IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal

Top referenced anti-FYN antibody for Flow Cytometry

  • Human Monoclonal FYN Primary Antibody for ICC, FACS - ABIN969159 (3 Publications): Brignatz, Paronetto, Opi, Cappellari, Audebert, Feuillet, Bismuth, Roche, Arold, Sette, Collette: Alternative splicing modulates autoinhibition and SH3 accessibility in the Src kinase Fyn. in Molecular and cellular biology 2009 (PubMed)

  • Human Monoclonal FYN Primary Antibody for FACS, IF - ABIN966166 (2 Publications): Lu, Zhu, Cvrljevic, Huang, Sarkaria, Ahkavan, Dang, Dinca, Plaisier, Oderberg, Lee, Chen, Caldwell, Xie, Loo, Seligson, Chakravari, Lee, Weinmann, Cloughesy, Nelson, Bergers, Graeber, Furnari, James, Cavenee, Johns, Mischel: Fyn and SRC are effectors of oncogenic epidermal growth factor receptor signaling in glioblastoma patients. in Cancer research 2009 (PubMed)

Similar anti-FYN Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 99 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 19 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunohistochemistry (IHC) 66 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunoprecipitation (IP) 40 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 211 Antibodies

General

Antigen FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
Reactivity Human
(220), (123), (39), (7), (7), (7), (2), (1), (1), (1), (1)
Host Mouse
(131), (97), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FYN antibody is un-conjugated
(9), (9), (8), (8), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(224), (113), (64), (39), (38), (27), (26), (18), (6), (4), (2), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-FYN Antibody

Target Details FYN Application Details Handling References for anti-FYN Antibody (ABIN969159) Images
Purification Ascites
Immunogen Purified recombinant fragment of human FYN expressed in E. coli.
Clone 2A10
Isotype IgG1

Target Details FYN

Product Details anti-FYN Antibody Application Details Handling References for anti-FYN Antibody (ABIN969159) Images back to top
Antigen
Alternative Name FYN (FYN Antibody Abstract)
Background This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
Molecular Weight 61 kDa
Gene ID 2534
HGNC 2534
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FYN Antibody Target Details FYN Handling References for anti-FYN Antibody (ABIN969159) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-FYN Antibody Target Details FYN Application Details References for anti-FYN Antibody (ABIN969159) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-FYN Antibody (ABIN969159)

Product Details anti-FYN Antibody Target Details FYN Application Details Handling Images back to top
Product cited in:

Brignatz, Paronetto, Opi, Cappellari, Audebert, Feuillet, Bismuth, Roche, Arold, Sette, Collette: "Alternative splicing modulates autoinhibition and SH3 accessibility in the Src kinase Fyn." in: Molecular and cellular biology, Vol. 29, Issue 24, pp. 6438-48, 2009

Lu, Zhu, Cvrljevic, Huang, Sarkaria, Ahkavan, Dang, Dinca, Plaisier, Oderberg, Lee, Chen, Caldwell, Xie, Loo, Seligson, Chakravari, Lee, Weinmann, Cloughesy, Nelson, Bergers, Graeber, Furnari, James, Cavenee, Johns, Mischel: "Fyn and SRC are effectors of oncogenic epidermal growth factor receptor signaling in glioblastoma patients." in: Cancer research, Vol. 69, Issue 17, pp. 6889-98, 2009

Kitkumthorn, Yanatatsanajit, Kiatpongsan, Phokaew, Triratanachat, Trivijitsilp, Termrungruanglert, Tresukosol, Niruthisard, Mutirangura: "Cyclin A1 promoter hypermethylation in human papillomavirus-associated cervical cancer." in: BMC cancer, Vol. 6, pp. 55, 2006

Images

Product Details anti-FYN Antibody Target Details FYN Application Details Handling References for anti-FYN Antibody (ABIN969159) back to top
Supplier Images
ELISA image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (ABIN969159) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (ABIN969159) Figure 2: Flow cytometric analysis of Hela cells using FYN mouse mAb (green) and nega...
Immunocytochemistry (ICC) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (ABIN969159) Figure 3: Immunofluorescence analysis of U251 cells using FYN mouse mAb (green) . Blu...
Immunohistochemistry (IHC) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (ABIN969159) Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (ABIN969159) Figure 5: Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombina...