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Rat (Rattus) IL-13 ELISA Kit

Recommended IL-13 Product (supplied by: Log in to see )

Antigen
Interleukin 13 (IL13) ELISA Kits
  • Il-13
  • IL-13
  • IL13
  • L13
  • P600
Epitope
AA 19-131
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
52
30
20
10
7
7
6
5
4
4
3
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921119
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN416369 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see 2
0.32693866 ABIN1115616 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN2708656 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32693866 ABIN2114265 Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) Log in to see
0.0012117105 ABIN365260 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012117105 ABIN627191 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012117105 ABIN625198 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids Log in to see
0.0012117105 ABIN1570620 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.813-500 pg/mL 7.813 pg/mL Log in to see
0.0012117105 ABIN1112725 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2042523 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2684582 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN1846947 Tissue Homogenate, Cell Lysate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2116077 0.625-40 pg/mL 0.625 pg/mL Log in to see
0.0012117105 ABIN579325 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2535387 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012117105 ABIN2088657 Sandwich ELISA Log in to see
0.0012117105 ABIN2116080 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2948866 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN1720601 Log in to see

Top referenced IL-13 ELISA Kit for ELISA

Similar IL-13 ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 20 ELISA Kits

General

Antigen Interleukin 13 (IL13) ELISA Kits
Epitope AA 19-131
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(52), (30), (20), (10), (7), (7), (6), (5), (4), (4), (3), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 3 references available
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Product Details IL-13 ELISA Kit

Target details Application Details Handling References for IL-13 Kit (ABIN921119) Images
Purpose For quantitative detection of rat IL-13 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant rat IL-13
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- rat IL-13 antibod - 1
  • Lyophilized recombinant rat IL-13 standar - 10ng/tubex2
  • Biotinylated anti- rat IL-13 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: T19-H131

Target details

Product Details IL-13 ELISA Kit Application Details Handling References for IL-13 Kit (ABIN921119) Images back to top
Antigen
Alternative Name IL13 (IL13 ELISA Kit Abstract)
Background Interleukin 13(IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2(Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor(IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6(STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.
Gene ID 116553
UniProt P42203
Research Area Cancer, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details IL-13 ELISA Kit Target details Handling References for IL-13 Kit (ABIN921119) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, T19-H131) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-13 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat IL-13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IL-13 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 174, Standard deviation: 7.66, CV(%): 4.4
  • Sample 2: n=16, Mean(pg/ml): 396, Standard deviation: 24.95, CV(%): 6.3
  • Sample 3: n=16, Mean(pg/ml): 648, Standard deviation: 35, CV(%): 5.4,
  • Sample 1: n=24, Mean(pg/ml): 284, Standard deviation: 14.77, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 520, Standard deviation: 39, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 706, Standard deviation: 46.6, CV(%): 6.6
Restrictions For Research Use only

Handling

Product Details IL-13 ELISA Kit Target details Application Details References for IL-13 Kit (ABIN921119) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL-13 Kit (ABIN921119)

Product Details IL-13 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Wynn: "IL-13 effector functions." in: Annual review of immunology, Vol. 21, pp. 425-56, 2003

Wills-Karp, Luyimbazi, Xu, Schofield, Neben, Karp, Donaldson: "Interleukin-13: central mediator of allergic asthma." in: Science (New York, N.Y.), Vol. 282, Issue 5397, pp. 2258-61, 1999

Morgan, Dolganov, Robbins, Hinton, Lovett: "The selective isolation of novel cDNAs encoded by the regions surrounding the human interleukin 4 and 5 genes." in: Nucleic acids research, Vol. 20, Issue 19, pp. 5173-9, 1992

Images

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Supplier Images
ELISA image for IL-13 ELISA Kit (Interleukin 13) (ABIN921119) Rat IL-13 PicoKine ELISA Kit standard curve