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anti-Human IL12 antibody for ELISA (Capture)

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • CLMF
  • CLMF2
  • IL-12 p40
  • Il-12a
  • IL-12B
  • IL-12p35
  • IL-12p40
  • Il12
  • il12b
  • IL12B
  • il12p40.b
  • Ll12a
  • LOC100217488
  • NKSF
  • NKSF2
  • p35
  • zgc:152789
Reactivity
Human
120
60
16
5
3
2
2
2
1
1
1
Host
Mouse
76
46
41
34
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is un-conjugated
30
11
8
7
6
4
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture)
126
114
43
32
25
21
11
10
9
8
7
7
7
6
4
4
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN281651
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086944667 ABIN1722430 ELISA (Capture) ELISPOT (Capture) Mouse IgG1 Log in to see B-T21
0.00086944667 ABIN1722431 ELISA (Capture) Mouse IgG1 Log in to see B-P40

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General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Human
(120), (60), (16), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(76), (46), (41), (34), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is un-conjugated
(30), (11), (8), (7), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture)
(126), (114), (43), (32), (25), (21), (11), (10), (9), (8), (7), (7), (7), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling Images
Specificity The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells. For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended.
Characteristics The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells. For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended.
Synonyms: Synonyms: Interleukin-12, IL12, p35
Purification Affinity Purified
Clone B-T21 (BT21)
Isotype IgG1

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-12 (IL12 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling Images back to top
Application Notes Applications reported: The B-T21 antibody has been reported for use as capture antibody in an IL-12 p70 sandwich ELISA.

Applications tested: The B-T21 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the biotinylated C8.6 (13-7129) antibody for detection and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C