Mouse (Murine) JAK1 ELISA Kit

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Antigen
Janus Kinase 1 (JAK1) ELISA Kits
  • JNK
  • JNK1
  • JAK1
  • jak1
  • JAK1A
  • JAK1B
  • JTK3
  • AA960307
  • C130039L05Rik
  • wu:fb93e10
  • Janus kinase 1
  • mitogen-activated protein kinase 8
  • Tyrosine-protein kinase Jak1
  • JAK1
  • MAPK8
  • jak1
  • Jak1
Epitope
pThr246, total
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
18
8
4
Method Type
Sandwich ELISA
Application
ELISA
Options
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Catalog No. ABIN4886153
$ 628.10
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2748290 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN1380618 Cell ELISA Log in to see
1 ABIN2114352 Cell ELISA Log in to see
1 ABIN1164602 Log in to see

General

Antigen Janus Kinase 1 (JAK1) ELISA Kits
Epitope pThr246, total
Reactivity Human, Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
(18), (8), (4)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details JAK1 ELISA Kit

Target details Application Details Handling Images
Purpose Human Phospho-PRAS40 (T246) & Total PRAS40 ELISA Kit. This assay semi-quantitatively measures phophorylated PRAS40 (Thr246) & Total PRAS40 in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Detection Method Colorimetric
Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes human JAK1 phosphorylated at site Tyrosine-1022 and total JAK1
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details JAK1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name JAK1 (JAK1 ELISA Kit Abstract)
Gene ID 3716
UniProt P23458
Research Area Kinases/Phosphatases, Signaling, Receptors, Cytokines
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded protein response

Application Details

Product Details JAK1 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details JAK1 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months