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anti-Mouse (Murine) Leptin antibody for Western Blotting

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 74-109, Middle Region
31
23
18
15
15
15
11
6
6
6
6
6
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
410
166
146
24
8
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
352
207
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
23
17
11
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Western Blotting (WB)
404
398
98
56
46
39
31
26
21
19
17
10
10
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN3043294
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0997953 ABIN2776944 IHC WB Rabbit Middle Region Log in to see Polyclonal
3.0997953 ABIN2467855 WB Chicken full length Log in to see Polyclonal
3.0997953 ABIN152764 ICC IHC IHC (fro) WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN223581 WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN1876874 IHC WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN1169154 IP ELISA WB Biotin Rabbit Log in to see Polyclonal
3.0997953 ABIN1078272 ICC IHC IP WB Rabbit IgG AA 22-167 Log in to see Polyclonal
3.0997953 ABIN3201294 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-167 Log in to see
3.0997953 ABIN4904218 IHC WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN634794 WB Rabbit N-Term Log in to see Polyclonal
3.0997953 ABIN634796 WB Rabbit Middle Region Log in to see Polyclonal
3.0997953 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.0997953 ABIN2406454 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2776943 IHC WB Rabbit N-Term Log in to see Polyclonal
1 ABIN115496 EIA WB Rabbit Log in to see Polyclonal 6
1 ABIN604738 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN604739 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN115975 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115976 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115977 EIA IHC (p) WB Rabbit Log in to see Polyclonal

Similar anti-Leptin Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 74-109, Middle Region
(31), (23), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(410), (166), (146), (24), (8), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(352), (207), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (23), (17), (11), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Western Blotting (WB)
(404), (398), (98), (56), (46), (39), (31), (26), (21), (19), (17), (10), (10), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Leptin(LEP) detection. Tested with WB, ELISA in Mouse.
Sequence KMDQTLAVYQ QVLTSLPSQN VLQIANDLEN LRDLLH
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Leptin(LEP) detection. Tested with WB, ELISA in Mouse.
Gene Name: leptin
Protein Name: Leptin
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin (74-109aa KMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLH), different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.

Synonyms: FLJ94114 antibody|LEP antibody|LEP_HUMAN antibody|LEPD antibody|Leptin (murine obesity homolog) antibody|Leptin (obesity homolog, mouse) antibody|Leptin antibody|Leptin Murine Obesity Homolog antibody|Leptin Precursor Obesity Factor antibody|OB antibody|Obese Protein antibody|Obese, mouse, homolog of antibody|Obesity antibody|Obesity factor antibody|Obesity factor antibody|Obesity homolog mouse antibody|Obesity Murine Homolog Leptin antibody|OBS antibody|OTTHUMP00000212285 antibody
Gene ID 16846
UniProt P41160
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin antibody (LEP) (AA 74-109) (ABIN3043294) Observed bind size: 16KD