Leptin antibody (N-Term)

Catalog No. ABIN2776943

This Rabbit Polyclonal antibody specifically detects Leptin in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Dog, Goat, Rabbit, Sheep and Guinea Pig.It has been mentioned in 1 publication

Quick Overview for Leptin antibody (N-Term) (ABIN2776943)

Target: Leptin (LEP)

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Goat, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Leptin Antibody

Binding Specificity: N-Term

Purpose: LEP Antibody - N-terminal region

Sequence: MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 91%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%, Sheep: 91%

Characteristics: This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LEP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41697-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Leptin antibody (ABIN2776943)

Müller, Kowalewski, Reichler, Kollár, Balogh: "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." in: Reproduction in domestic animals = Zuchthygiene, Vol. 52 Suppl 2, pp. 187-192, (2017) (PubMed).

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: LEP

Background:

Background Information: This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development.

Gene Name: leptin

Alternative Symbols: OB, OBS, LEPD

Protein Name: leptin

Molecular Weight: 19kDa

Gene ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process