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anti-Rat (Rattus) Leptin antibody for Western Blotting

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
Middle Region
24
23
21
15
15
11
10
8
6
6
6
6
6
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
419
159
137
20
3
2
2
1
Host
Rabbit
360
213
20
8
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
65
23
17
11
7
7
7
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
409
387
91
55
40
39
30
26
19
17
16
10
9
7
5
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2776944
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.022232 ABIN1169158 ELISA WB Mouse IgG2a Log in to see RLEP 227
3.022232 ABIN152764 ICC IHC IHC (fro) WB Rabbit IgG Log in to see Polyclonal
3.022232 ABIN2467855 WB Chicken full length Log in to see Polyclonal
3.022232 ABIN1876874 IHC WB Rabbit IgG Log in to see Polyclonal
3.022232 ABIN1169153 ELISA WB Biotin Rabbit Log in to see Polyclonal
3.022232 ABIN1078273 ICC IHC IP WB Rabbit IgG AA 22-167 Log in to see Polyclonal
3.022232 ABIN1169150 ELISA WB Rabbit Log in to see Polyclonal
3.022232 ABIN634796 WB Rabbit Middle Region Log in to see Polyclonal
3.022232 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.022232 ABIN2406454 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN115507 EIA WB Rabbit Log in to see Polyclonal 7
1 ABIN725030 IF (p) IHC (p) WB Rabbit IgG AA 90-130 Log in to see Polyclonal
1 ABIN668243 IF (p) IHC (p) WB Rabbit IgG AA 1-50 Log in to see Polyclonal
1 ABIN1585862 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1824733 WB Rabbit IgG Log in to see Polyclonal
1 ABIN108522 ELISA WB Rabbit Log in to see Polyclonal 6
1 ABIN108521 ELISA WB Rabbit Log in to see Polyclonal 4
1 ABIN411526 WB Rabbit IgG Log in to see Polyclonal
1 ABIN1169161 ELISA WB Mouse IgG2a Log in to see RLEP 227
1 ABIN465028 ELISA WB Goat Log in to see Polyclonal

Similar anti-Leptin Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Western Blotting (WB) 95 Antibodies 258 Antibodies 77 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies 2 Antibodies 2 Antibodies
Protein Assay (PrA) 2 Antibodies
Neutralization (Neut) 2 Antibodies 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 20 Antibodies 7 Antibodies 1 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 44 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope Middle Region
(24), (23), (21), (15), (15), (11), (10), (8), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(419), (159), (137), (20), (3), (2), (2), (1)
Host Rabbit
(360), (213), (20), (8), (4)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(65), (23), (17), (11), (7), (7), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(409), (387), (91), (55), (40), (39), (30), (26), (19), (17), (16), (10), (9), (7), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN979273
Sequence LHVLAFSKSC HLPWASGLET LDSLGGVLEA SGYSTEVVAL SRLQGSLQDM
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Goat : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Sheep : 100 %, Pig : 90 %, Rabbit : 90 %, Dog : 70 %
Characteristics This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human LEP

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEP (LEP Antibody Abstract)
Background LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Molecular Weight 16 kDa
Gene ID 3952
NCBI Accession NM_000230, NP_000221
UniProt P41159
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 167 AA

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin antibody (LEP) (Middle Region) (ABIN2776944) WB Suggested Anti-LEP Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...
 image for anti-Leptin antibody (LEP) (Middle Region) (ABIN2776944) anti-Leptin (LEP) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Leptin antibody (LEP) (Middle Region) (ABIN2776944) anti-Leptin (LEP) (Middle Region) antibody (Image 3)