Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription. Western Blot (Ab 1-2 Î¼/mL for 2hrs at RT) Immunohistology (Formalin/paraffin) (Use Ab at 1-2 Î¼/mL for 30â€¯min at RT) (Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1â€¯mg/mL Tris-HCl,â€¯pH 2.0 for 15â€¯min at RT or 10â€¯min at 37 deg C) Positive Control Breast carcinoma Cellular Localization Cell membrane/cytoplasmic Recognizes Prolactin Receptor. Species Crossreactivity: Human.