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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||137 Antibodies||48 Antibodies||45 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies||4 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies||12 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||56 Antibodies||40 Antibodies||36 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies||22 Antibodies||22 Antibodies|
|Immunofluorescence (IF)||29 Antibodies||17 Antibodies||16 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Antigen||Protein-tyrosine Phosphatase 1C (PTPN6) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PTPN6 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|4 references available|
|Supplier||Log in to see|
Product Details anti-PTPN6 AntibodyTarget Details PTPN6 Application Details Handling References for anti-PTPN6 Antibody (ABIN319255) Images
|Specificity||This Antibody AP08023PU detects endogenous levels of PTPN6/SHP-1 only when phosphorylated at Tyrosine 536.|
|Purification||Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.|
|Immunogen||The antiserum was produced against synthesized phosphopeptide derived from human SHP-1 around the phosphorylation site of Tyrosine 536 (S-E-YP-G-N).|
Target Details PTPN6Product Details anti-PTPN6 Antibody Application Details Handling References for anti-PTPN6 Antibody (ABIN319255) Images back to top
|Alternative Name||PTPN6 (PTPN6 Antibody Abstract)|
|Background||SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer.Synonyms: HCP, Hematopoietic cell protein-tyrosine phosphatase, PTP-1C, PTP1C, PTPN-6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, Tyrosine-protein phosphatase non-receptor type 6|
|Pathways||JAK-STAT Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway|
Application DetailsProduct Details anti-PTPN6 Antibody Target Details PTPN6 Handling References for anti-PTPN6 Antibody (ABIN319255) Images back to top
Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PTPN6 Antibody Target Details PTPN6 Application Details References for anti-PTPN6 Antibody (ABIN319255) Images back to top
|Buffer||PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at-20 °C.|
References for anti-PTPN6 Antibody (ABIN319255)Product Details anti-PTPN6 Antibody Target Details PTPN6 Application Details Handling Images back to top
Hauck, Gulbins, Lang et al.: "Tyrosine phosphatase SHP-1 is involved in CD66-mediated phagocytosis of Opa52-expressing Neisseria gonorrhoeae." in: Infection and immunity, Vol. 67, Issue 10, pp. 5490-4, 1999 (PubMed).
Kanagasundaram, Jaworowski, Byrne et al.: "Separation and characterization of the activated pool of colony-stimulating factor 1 receptor forming distinct multimeric complexes with signalling molecules in macrophages." in: Molecular and cellular biology, Vol. 19, Issue 6, pp. 4079-92, 1999 (PubMed).
Timms, Carlberg, Gu et al.: "Identification of major binding proteins and substrates for the SH2-containing protein tyrosine phosphatase SHP-1 in macrophages." in: Molecular and cellular biology, Vol. 18, Issue 7, pp. 3838-50, 1998 (PubMed).
Migone, Cacalano, Taylor et al.: "Recruitment of SH2-containing protein tyrosine phosphatase SHP-1 to the interleukin 2 receptor; loss of SHP-1 expression in human T-lymphotropic virus type I-transformed T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3845-50, 1998 (PubMed).
ImagesProduct Details anti-PTPN6 Antibody Target Details PTPN6 Application Details Handling References for anti-PTPN6 Antibody (ABIN319255) back to top