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Mouse (Murine) STAT1 ELISA Kit

Recommended STAT1 Product (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA Kits
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Epitope
pTyr701
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
27
25
12
2
1
1
1
1
Methode Type
Cell ELISA
Application
ELISA
Supplier
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Catalog No. ABIN1981837
$ 446.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012085857 ABIN625231 Sandwich ELISA Cell Lysate Log in to see
0.0012085857 ABIN625230 Sandwich ELISA Cell Lysate Log in to see
0.0012085857 ABIN625243 Sandwich ELISA Cell Lysate Log in to see
0.0012085857 ABIN625244 Sandwich ELISA Cell Lysate Log in to see
0.0012085857 ABIN1117106 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.0012085857 ABIN1380703 Cell ELISA Log in to see
0.0012085857 ABIN1380704 Cell ELISA Log in to see
0.0012085857 ABIN510243 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012085857 ABIN1381518 Cell ELISA Log in to see
0.0012085857 ABIN2114923 Log in to see
0.0012085857 ABIN2114922 Cell ELISA Log in to see
0.0012085857 ABIN2114924 Log in to see
0.0012085857 ABIN2114921 Cell ELISA Log in to see
0.0012085857 ABIN2851541 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.0012085857 ABIN2114925 Log in to see
0.0012085857 ABIN2685531 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
0.0012085857 ABIN2685532 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
0.0012085857 ABIN2114920 Cell ELISA Log in to see
0.0012085857 ABIN2685534 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
0.0012085857 ABIN2685535 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see

Top referenced STAT1 ELISA Kit for ELISA

  • Human STAT1 ELISA Kit for Cell ELISA - ABIN1981837 (1 Publications): Nakano, Fujii, Hashimoto, Yukawa, Yoshifuji, Ohmura, Nakaizumi, Mimori: Type I interferon induces CX3CL1 (fractalkine) and CCL5 (RANTES) production in human pulmonary vascular endothelial cells. in Clinical and experimental immunology 2012 (PubMed)

Similar STAT1 ELISA Kits

Application / Reactivity Human Mouse (Murine)
ELISA 28 ELISA Kits 26 ELISA Kits

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA Kits
Epitope pTyr701
Reactivity Human, Mouse (Murine)
Kits with alternative reactivity to:
(27), (25), (12), (2), (1), (1), (1), (1)
Methode Type Cell ELISA
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details STAT1 ELISA Kit

Target details Application Details Handling References for STAT1 Kit (ABIN1981837) Images
Purpose Semi-Quantitative Cell-Based Human/Mouse Stat 1 (Tyr701) Phosphorylation ELISA Kit for adherent whole cell lines.
Brand CellBIND®
Sample Type Adherent Cell Culture
Detection Method Colorimetric
Specificity The antibody pair provided in this kit recognizes human and mouse Stat 1 (pTyr701).
Characteristics Semi-Quantitative
Components 96 wells (12 strips x 8 wells) Coated With Antibody
Wash Buffer Concentrate (20x)
Positive Control
Assay Diluent
Detection Antibody
Secondary Antibody or HRP Streptavidin
TMB One-Step Substrate Reagent
Stop Solution
Cell Lysis Buffer
Material not included Distilled or deionized water
100 mL and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details STAT1 ELISA Kit Application Details Handling References for STAT1 Kit (ABIN1981837) Images back to top
Antigen
Alternative Name STAT1 (STAT1 ELISA Kit Abstract)
Background STAT1
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details STAT1 ELISA Kit Target details Handling References for STAT1 Kit (ABIN1981837) Images back to top
Comment

Site and signal pathway-specificIn vitro detection of adherent cell cultureNo sample lysis neededCompatible with a standard ELISA plate readerFaster results than with ELISAAdaptable for high-throughput screening and drug discovery

Plate 96 wells
Protocol 1. Add cells
2. Treatment with stimulators or inhibitors
3. Fixing and blocking
4. Anti-phospho-protein antibody or anti-pan-protein antibody
5. HRP-conjugated secondary antibody
6. Develop with substrate
Reagent Preparation NOTE: Thaw all reagents to room temperature immediately before use. If wash buffers contain visible crystals, warm to room temperature and mix gently until dissolved.
NOTE: Briefly centrifuge (~1,000g) ITEMS G, H, and I before opening to ensure maximum recovery.
For more information look at the picture.
Assay Procedure NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1-2 cycles/sec).
1. Design your experiment. For example, see Figure 2 below.
OPTIONAL: If seeding HUVECs, HMEC-1 or other loosely attached cells, coat the Uncoated 96-Well Microplate (ITEM A) by adding 100 µL poly-L-Lysine (Recommended Sigma Aldrich) into each well and then follow manufacturer's instructions. A pre-coated CellBIND® microplate or other poly-lysine treated tissue culture plate may be used in place of Item A.
2. Seed 100 µL of 30,000 cells into each well of the Uncoated 96-Well Microplate (ITEM A) provided and incubate overnight at 37 °C with 5 % CO2.
NOTE: The optimal cell number used will vary on the cell line and the relative amount of protein phosphorylation. More or less cells may be used but this must be determined empirically.
NOTE: The cells can be starved ~4-24 hours (depending on cell line) prior to treatment with inhibitors or activators.
3. Apply various treatments, inhibitors (such as siRNA or chemicals) or activators according to manufacturer's instructions and incubate for the desired time points.
NOTE: It is recommended to dissolve inhibitors or activators into serum-free cell culture medium before treating the cells (unless otherwise stated in the manufacturer's instructions.)
4. Discard the cell culture medium by flipping the microplate upside down and gently tapping the bottom of the microplate over a sink.
5. Wash by pipetting 200 µL of the prepared 1X Wash Buffer A (ITEM B) into each well. Discard the wash buffer (same as step 4) and wash 2 more times for a total of 3 washes using fresh wash buffer each time. After the final wash, gently blot the microplate onto a paper towel to remove any excess/remaining buffer.
NOTE: To avoid cell loss, do not pipette directly onto the cells. Instead, gently dispense the liquid down the wall of cell culture wells. Also avoid the use of vacuum suction or too forcefully tapping the microplate when discarding any solution.
6. Add 100 µL of Fixing Solution (ITEM D) into each well and incubate for 20 minutes at room temperature.
NOTE: The fixing solution is used to permeabilize the cells.
7. Repeat wash step 5.
8. Add 200 µL of the prepared 1X Quenching Buffer (ITEM E) into each well and incubate 20 minutes at room temperature.
NOTE: The quenching buffer is used to minimize the background response.
9. Wash 4 times with 1X Wash Buffer A.
10. Add 200 µL of the prepared 1X Blocking Buffer (ITEM F) into each well and incubate for 1 hour at 37 °C.
11. Wash 3 times with the prepared 1X Wash Buffer B (ITEM C).
NOTE: If needed, the microplate may be stored at -80 °C for several days after this wash.
12. Add 50 µL of the prepared 1X primary antibody (ITEM G or H) into each corresponding well and incubate for 2 hours at room temperature.
13. Wash 4 times with 1X Wash Buffer B.
14. Add 50 µL of 1X HRP Conjugated secondary antibody (ITEM I) into each well and incubate for 1 hour at room temperature.
15. Wash 4 times with 1X Wash Buffer B.
16. Add 100 µL of the TMB Substrate (ITEM J) into each well and incubate for 30 minutes at room temperature in the dark.
17. Add 50 µL of the Stop Solution (ITEM K) into each well. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details STAT1 ELISA Kit Target details Application Details References for STAT1 Kit (ABIN1981837) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Store entire kit at ≤ -20 °C immediately upon arrival.
Expiry Date 6 months

References for STAT1 Kit (ABIN1981837)

Product Details STAT1 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Nakano, Fujii, Hashimoto, Yukawa, Yoshifuji, Ohmura, Nakaizumi, Mimori: "Type I interferon induces CX3CL1 (fractalkine) and CCL5 (RANTES) production in human pulmonary vascular endothelial cells." in: Clinical and experimental immunology, Vol. 170, Issue 1, pp. 94-100, 2012

Images

Product Details STAT1 ELISA Kit Target details Application Details Handling References for STAT1 Kit (ABIN1981837) back to top
Supplier Images
 image for STAT1 ELISA Kit (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN1981837) Cell-Based protein phosphorylation procedure
 image for STAT1 ELISA Kit (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN1981837) This picture shows the reagent preparation.
 image for STAT1 ELISA Kit (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN1981837) Example of how to seed cells for cell-based assay
 image for STAT1 ELISA Kit (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN1981837) A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37 °C
 image for STAT1 ELISA Kit (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN1981837) A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37 °C.
Western Blotting (WB) image for STAT1 ELISA Kit (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN1981837) Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells. Phospho-sta...