STAT1 ELISA Kit

Catalog No. ABIN4886306

Product Details STAT1 ELISA Kit

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Binding Specificity: pTyr

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Phosphotyrosine STAT1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine STAT1 in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-STAT1.

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT 1 (STAT1 Products)

Synonyms: CANDF7 ELISA Kit, ISGF-3 ELISA Kit, STAT91 ELISA Kit, 2010005J02Rik ELISA Kit, AA408197 ELISA Kit, XStat1 ELISA Kit, STAT4 ELISA Kit, DD6G4-4 ELISA Kit, STAT ELISA Kit, STAT1 ELISA Kit, dZ182H3.3 ELISA Kit, si:by134g18.2 ELISA Kit, si:by51f19.1 ELISA Kit, si:dz182h3.3 ELISA Kit, si:dz199m19.1 ELISA Kit, si:dz73o4.2 ELISA Kit, stat1 ELISA Kit, signal transducer and activator of transcription 1 ELISA Kit, signal transducer and activator of transcription 1 L homeolog ELISA Kit, signal transducer and activator of transcription 1, 91kDa ELISA Kit, signal transducer and activator of transcription 4 ELISA Kit, signal transducer and activator of transcription 1a ELISA Kit, STAT1 ELISA Kit, Stat1 ELISA Kit, stat1.L ELISA Kit, stat4 ELISA Kit, stat1a ELISA Kit

Gene ID: 6772

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events