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anti-Human STAT3 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
AA 50-240
107
94
84
28
21
17
16
15
14
13
13
8
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
519
248
192
30
24
23
12
11
10
5
4
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
452
97
34
4
1
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
24
22
18
13
12
11
7
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
485
233
206
74
69
64
46
25
23
13
12
12
9
7
3
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1027558
$ 186.72
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008736138 ABIN1027559 IHC (f) IHC (p) Rabbit IgG AA 50-240 Log in to see Polyclonal

Similar anti-STAT3 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 199 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 18 Antibodies
Gel Shift (GS) 2 Antibodies
Immunoassay (IA) 2 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 38 Antibodies
Immunodiffusion (ID) 1 Antibodies
Immunofluorescence (IF) 72 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 196 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 61 Antibodies
Immunoprecipitation (IP) 53 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope AA 50-240
(107), (94), (84), (28), (21), (17), (16), (15), (14), (13), (13), (8), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(519), (248), (192), (30), (24), (23), (12), (11), (10), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(452), (97), (34), (4), (1), (1)
Clonality
Polyclonal
Conjugate This STAT3 antibody is un-conjugated
(24), (22), (18), (13), (12), (11), (7), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(485), (233), (206), (74), (69), (64), (46), (25), (23), (13), (12), (12), (9), (7), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling Images
Purpose This antibody is designed for the specific localization of human STAT3 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Characteristics STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway - a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Purification Ready-to-use, Prediluted,
Material not included All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope. Anti-STAT3 Rabbit polyclonal antibody
Immunogen Recombinant protein encoding human STAT3 aa 50-240.
Isotype IgG

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Comment

Staining pattern: Nuclear, cytoplasmic. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from breast carcinoma.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for STAT3.

Protocol Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended
Assay Procedure Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:50 – 1:200
Incubation: 30 min, RT
Control Tissue: Breast carcinoma
Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.