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|Application / Reactivity||Mouse (Murine)||Human|
|Chromatin Immunoprecipitation (ChIP)||3 Antibodies||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||36 Antibodies|
|Gel Shift (GS)||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||33 Antibodies|
|Immunofluorescence (IF)||58 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||27 Antibodies|
|Immunohistochemistry (IHC)||120 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||37 Antibodies|
|Immunoprecipitation (IP)||53 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Intracellular Staining (ICS)||12 Antibodies|
|Western Blotting (WB)||212 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine) Alternatives|
|Conjugate||This STAT3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-STAT3 AntibodyTarget Details STAT3 Application Details Handling Images
|Specificity||The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1. AM00148PU-N specifically recognizes STAT3 phosphorylated at Tyr 705 in human, mouse and dog. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins. AM00148PU-N is particularly suitable for Western blot and ELISA applications.|
|Characteristics||Synonyms: STAT-3, Acute-phase response factor, APRF, Signal transducer and activator oftranscription 3|
|Purification||Size exclusion chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic phosphopeptide conjuagted to KLH|
Target Details STAT3Product Details anti-STAT3 Antibody Application Details Handling Images back to top
|Alternative Name||STAT3 (STAT3 Antibody Abstract)|
|Background||The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3|
|Molecular Weight||92 kDa|
|Research Area||Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus|
Application DetailsProduct Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10g per 106 pervanadate treated HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS with 0.09 % Sodium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
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