anti-Mouse (Murine) STAT3 antibody for Western Blotting

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • AW109958
  • Aprf
  • APRF
  • HIES
  • Xstat3
  • aprf
  • hies
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • z-Stat3
  • signal transducer and activator of transcription 3 (acute-phase response factor)
  • signal transduction and activation of transcription 3
  • signal transducer and activator of transcription 3
  • signal transducer and activator of transcription 3 (acute-phase response factor), gene 1
  • STAT3
  • stat3
  • Stat3
  • stat3.1
Epitope
N-Term
113
89
72
25
18
17
17
16
15
15
13
11
6
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
589
344
272
40
36
32
29
16
10
9
7
5
4
4
4
3
3
2
1
1
1
1
1
Host
Rabbit
458
136
34
5
2
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
28
23
18
14
11
11
9
6
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
540
243
215
92
80
80
58
54
27
12
11
9
8
6
5
3
3
3
2
2
2
2
1
1
1
1
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Catalog No. ABIN2780479
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3471746 ABIN2779321 WB Rabbit Middle Region Log in to see Polyclonal 1
3.3471746 ABIN2792239 IHC WB Rabbit Middle Region Log in to see Polyclonal 2
3.3471746 ABIN1449165 WB Mouse IgG1 AA 703-714, pTyr708, pTyr705 Log in to see PS3-1 1
3.3471746 ABIN1531975 IHC ELISA WB Rabbit IgG pSer727 Log in to see Polyclonal 1
3.3471746 ABIN389664 IF IHC (p) WB Rabbit Ig pSer727 Log in to see Polyclonal 3
3.3471746 ABIN1533010 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.3471746 ABIN1533722 ELISA WB Rabbit IgG Log in to see Polyclonal 3
3.3471746 ABIN967809 IF IP IHC WB Mouse IgG1 AA 1-175 Log in to see 84-Stat3 5
3.3471746 ABIN968833 ICS WB Mouse IgG2a, kappa pTyr705 Log in to see 4-P 4
3.3471746 ABIN968868 IF WB Mouse IgG1 pSer727 Log in to see 49-p 4
3.3471746 ABIN968869 IF WB Mouse IgG1 pSer727 Log in to see 49-p 4
3.3471746 ABIN967808 IF IP IHC WB Mouse IgG1 AA 1-175 Log in to see 84-Stat3 5
3.3471746 ABIN968834 ICS WB Mouse IgG2a, kappa pTyr705 Log in to see 4-P 4
3.3471746 ABIN3043299 WB Rabbit IgG AA 139-167, N-Term Log in to see Polyclonal 8
3.3471746 ABIN256760 ICC IF IHC (p) IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
3.3471746 ABIN4356373 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
3.3471746 ABIN126898 EIA IHC (fro) IF IP WB Mouse IgG1 pTyr705 Log in to see 9-00E-012
3.3471746 ABIN969421 ICC IHC ELISA WB Mouse IgG1 Log in to see 3B5 1
3.3471746 ABIN4898973 FACS ICC WB Sheep IgG AA 1-175 Log in to see Polyclonal 1
3.3471746 ABIN4898974 FACS ICC IP WB Mouse IgG2b AA 1-175 Log in to see 232209 2

Top referenced anti-STAT3 antibody for Western Blotting

Similar anti-STAT3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Zebrafish Guinea Pig Horse (Equine) Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies 4 Antibodies 4 Antibodies 2 Antibodies 1 Antibodies
In Vivo Imaging (IVI) 1 Antibodies
Western Blotting (WB) 496 Antibodies 268 Antibodies 209 Antibodies 30 Antibodies 31 Antibodies 5 Antibodies 5 Antibodies 9 Antibodies 11 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope N-Term
(113), (89), (72), (25), (18), (17), (17), (16), (15), (15), (13), (11), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(589), (344), (272), (40), (36), (32), (29), (16), (10), (9), (7), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(458), (136), (34), (5), (2), (1)
Clonality
Polyclonal
Conjugate This STAT3 antibody is un-conjugated
(28), (23), (18), (14), (11), (11), (9), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(540), (243), (215), (92), (80), (80), (58), (54), (27), (12), (11), (9), (8), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling ProductDetails: References for anti-STAT3 antibody (ABIN2780479) Images
Blocking Peptide Blocking peptide for this product available: ABIN984529
Sequence AQWNQLQQLD TRYLEQLHQL YSDSFPMELR QFLAPWIESQ DWAYAASKES
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against STAT3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human STAT3

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling ProductDetails: References for anti-STAT3 antibody (ABIN2780479) Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Alias Symbols: APRF, FLJ20882, MGC16063, HIES
Protein Interaction Partner: MTOR, GADD45A, BMX, BLK, UBC, SYK, LMO2, SRI, HIC1, GNB2, USP22, RELA, COPS5, BRD4, ADRB2, PSMG3, TBCD, TBC1D15, PUS7, LUC7L2, ARFIP1, IVNS1ABP, MSH2, BMH1, SIN3A, EGFR, PIAS3, HDAC3, SRC, LYN, JAK3, JAK2, HES5, TRIM28, HES1, HCK, FYN, FGR, HNRNPM, SULT2A
Protein Size: 769
Molecular Weight 88 kDa
Gene ID 6774
NCBI Accession NM_003150, NP_003141
UniProt B5BTZ6
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling ProductDetails: References for anti-STAT3 antibody (ABIN2780479) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 769 AA

Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details ProductDetails: References for anti-STAT3 antibody (ABIN2780479) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-STAT3 antibody (ABIN2780479)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Product cited in:

Deng, Chen, Li, Yuan, Li, Ke, Wang, Wu, Luo: "Bone marrow mesenchymal stromal cells with CD47 high expression via the signal transducer and activators of transcription signaling pathway preventing myocardial fibrosis." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10555-64, 2015

Vega, Crotty Alexander, Charles, Hwang, Nizet, De Maio: "Activation of the stress response in macrophages alters the M1/M2 balance by enhancing bacterial killing and IL-10 expression." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 12, pp. 1305-17, 2014

Deng, Chen, Li, Liu, Niu, Zhou, Li, Ke, Mo et al.: "Bone marrow mesenchymal stromal cells with support of bispecific antibody and ultrasound-mediated microbubbles prevent myocardial fibrosis via the signal transducer and activators of transcription ..." in: Cytotherapy, Vol. 13, Issue 4, pp. 431-40, 2011

Background publications

Ohbayashi, Kawakami, Muromoto, Togi, Ikeda, Kamitani, Sekine, Honjoh, Matsuda: "The IL-6 family of cytokines modulates STAT3 activation by desumoylation of PML through SENP1 induction." in: Biochemical and biophysical research communications, Vol. 371, Issue 4, pp. 823-8, 2008

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling ProductDetails: References for anti-STAT3 antibody (ABIN2780479) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (N-Term) (ABIN2780479) WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (N-Term) (ABIN2780479) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (N-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (N-Term) (ABIN2780479) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (N-Term) antibody (Image 3)