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anti-Human STAT5A antibody for Immunohistochemistry (Frozen Sections)

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5A-like
  • signal transducer and activator of transcription 5B
  • STAT5A
  • Stat5a
  • LOC100348518
  • STAT5B
Epitope
pTyr695, pTyr699
93
50
45
17
16
15
9
6
6
6
5
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
446
239
210
35
23
19
17
17
7
3
2
1
1
1
1
Host
Mouse
337
116
12
Clonality
Monoclonal
Conjugate
This STAT5A antibody is un-conjugated
12
12
10
8
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
364
220
152
85
76
69
37
31
26
18
11
8
7
6
5
4
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN126900
$ 555.50
Plus shipping costs $45.00

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General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope pTyr695, pTyr699
(93), (50), (45), (17), (16), (15), (9), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(446), (239), (210), (35), (23), (19), (17), (17), (7), (3), (2), (1), (1), (1), (1)
Host Mouse
(337), (116), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(364), (220), (152), (85), (76), (69), (37), (31), (26), (18), (11), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling Images
Specificity This antibody specifically recognizes STAT5A phosphorylated at Tyr 694 and STAT5B phosphorylated at Tyr 699. The antibody does not crossreact with the non-phosphorylated form of STAT5 nor with unrelated tyrosine-phosphorylated proteins.
Characteristics Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A
Purification Size exclusion chromatography
Immunogen Synthetic phosphopeptide conjuagted to KLH
Clone 5G4
Isotype IgG1

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT5 / STAT5A (STAT5A Antibody Abstract)
Background The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT5 is activated in response to prolactine, growth hormone, GM-CSF, and IL-2.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Gene ID 6776
UniProt P42229
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Images back to top
Concentration 0.1mg/mL
Buffer PBS/0.09 % Na-Azide/PEG and Sucrose/50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody (Aliquote and freeze in liquid nitrogen) at -20 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr695) (ABIN126900) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr695), (pTyr699) antibody