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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Enzyme Immunoassay (EIA)||9 Antibodies|
|Flow Cytometry (FACS)||72 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||31 Antibodies|
|Immunofluorescence (IF)||66 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||211 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||78 Antibodies|
|Immunoprecipitation (IP)||30 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Intracellular Staining (ICS)||11 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies|
|Epitope||pTyr695, pTyr699 Alternatives|
|Conjugate||This STAT5A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
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Product Details anti-STAT5A AntibodyTarget Details STAT5A Application Details Handling Images
|Specificity||This antibody specifically recognizes STAT5A phosphorylated at Tyr 694 and STAT5B phosphorylated at Tyr 699. The antibody does not crossreact with the non-phosphorylated form of STAT5 nor with unrelated tyrosine-phosphorylated proteins.|
|Characteristics||Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A|
|Purification||Size exclusion chromatography|
|Immunogen||Synthetic phosphopeptide conjuagted to KLH|
Target Details STAT5AProduct Details anti-STAT5A Antibody Application Details Handling Images back to top
|Alternative Name||STAT5 / STAT5A (STAT5A Antibody Abstract)|
|Background||The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT5 is activated in response to prolactine, growth hormone, GM-CSF, and IL-2.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A|
|Pathways||JAK-STAT Signaling, RTK Signaling|
Application DetailsProduct Details anti-STAT5A Antibody Target Details STAT5A Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Images back to top
|Buffer||PBS/0.09 % Na-Azide/PEG and Sucrose/50 % Glycerol|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody (Aliquote and freeze in liquid nitrogen) at -20 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling back to top