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|Antigen||Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT5A antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-STAT5A AntibodyTarget Details STAT5A Application Details Handling Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412921|
The antibody recognizes ~95 kDa Stat5 of human, mouse and rat origins.
Reactivity to other species has not been tested.
|Immunogen||Synthetic peptide surrounding amino acid 777 of human Stat5|
Target Details STAT5AProduct Details anti-STAT5A Antibody Application Details Handling Images back to top
|Alternative Name||STAT5 (STAT5A Antibody Abstract)|
Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Althoμgh early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Synonyms: Signal transducer and activator of transcription 5, stat -5, STAT5, STAT-5, STAT 5
|Research Area||Cancer, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation|
Application DetailsProduct Details anti-STAT5A Antibody Target Details STAT5A Handling Images back to top
|Application Notes||Western blotting (2-10 μg/mL), immunoprecipitation (20 μg/mL) and Immunohistochemistry (5 μg/mL). However, the optimal concentrations should be determined individually. The antibody recognizes ~95 kDa Stat5 of human, mouse and rat origins. Reactivity to other species has not been tested.|
Suggested positive control:
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS).|
|Handling Advice||The antibody solution should be gently mixed before use.|
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
ImagesProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling back to top