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anti-Rat (Rattus) STAT5A antibody for Immunofluorescence

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • AA959963
  • MGF
  • STAT5
  • Stat5
  • STAT5A
  • STAT5A/MGF
  • STATA5
Epitope
Tyr694
86
52
42
17
14
13
9
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
405
194
156
28
21
16
13
11
6
1
Host
Mouse
322
96
10
Clonality
Monoclonal
Conjugate
This STAT5A antibody is un-conjugated
12
11
10
8
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
323
195
132
55
54
50
33
22
18
17
12
8
6
4
4
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN400812
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3200249 ABIN967810 IF IP IHC WB Mouse IgG2b AA 451-649 Log in to see 89-Stat5 5
0.3200249 ABIN967811 IF IP IHC WB Mouse IgG2b AA 451-649 Log in to see 89-Stat5 5
0.3200249 ABIN2707112 IC IF WB Rabbit Center Log in to see Polyclonal
0.001084523 ABIN272189 IF WB Rabbit Log in to see Polyclonal
0.001084523 ABIN223192 IF WB Rabbit IgG pTyr694 Log in to see Polyclonal
0.001084523 ABIN3187089 ELISA IF WB Rabbit IgG Internal Region Log in to see Polyclonal
0.001084523 ABIN1003513 IF WB Rabbit phosphospecific Log in to see Polyclonal
0.001084523 ABIN2964322 FACS IF IHC (p) WB Mouse IgG1 Log in to see 9F7
0.001084523 ABIN2670147 FACS IF IHC WB Mouse IgG1 Log in to see OTI9F7
0.001084523 ABIN2670149 FACS IF IHC WB Mouse IgG1 Log in to see OTI4E7

Top referenced anti-STAT5A antibody for Immunofluorescence

Similar anti-STAT5A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 37 Antibodies 50 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 4 Antibodies 9 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 83 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Western Blotting (WB) 113 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Gel Shift (GS) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope Tyr694
(86), (52), (42), (17), (14), (13), (9), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(405), (194), (156), (28), (21), (16), (13), (11), (6), (1)
Host Mouse
(322), (96), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is un-conjugated
(12), (11), (10), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(323), (195), (132), (55), (54), (50), (33), (22), (18), (17), (12), (8), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN400812) Images
Specificity This phospho-specific monoclonal antibody reacts with Stat5a pTyr694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.
Cross-Reactivity (Details) Species reactivity (expected):Human and Rat (100 %).
Species reactivity (tested):Mouse.
Characteristics Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A
Purification Protein A Chromatography from concentrated tissue culture supernate.
Immunogen Synthetic peptide corresponding to residues surrounding Tyr694 of Mouse STAT5a protein.
Clone 5F6-F1
Isotype IgG1

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN400812) Images back to top
Antigen
Alternative Name STAT5 / STAT5A (STAT5A Antibody Abstract)
Background Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Gene ID 20850
UniProt P42230
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN400812) Images back to top
Application Notes ELISA: 1/20,000. Western blot: 1/500-1/2,000 (Expect a band approximately 91 kDa in size corresponding tophosphorylated Stat5α protein in the appropriate cell lysate or extract). Immunohistochemistry (20-40 μg/mL). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN400812) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening. For low volume of reagent (25 μl), to minimizeloss of volume dilute 1/10 by adding 225 μl of the buffer stated abovedirectly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at thebottom of the vial. Use this intermediate dilution when calculating final dilutions asrecommended below. Store the vial at -20 °C or below after dilution. Avoid cycles of freezing and thawing.
Shelf life: One year from despatch (3 months from date of opening).
Expiry Date 12 months

References for anti-STAT5A Antibody (ABIN400812)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Background publications

Engblom, Kornfeld, Schwake, Tronche, Reimann, Beug, Hennighausen, Moriggl, Schütz: "Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression." in: Genes & development, Vol. 21, Issue 10, pp. 1157-62, 2007

Baugh, Floyd, Stephens: "The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes." in: Obesity (Silver Spring, Md.), Vol. 15, Issue 3, pp. 583-90, 2007

Laurence, Tato, Davidson, Kanno, Chen, Yao, Blank, Meylan, Siegel, Hennighausen, Shevach, Oshea: "Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation." in: Immunity, Vol. 26, Issue 3, pp. 371-81, 2007

Burchill, Yang, Vogtenhuber, Blazar, Farrar: "IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 1, pp. 280-90, 2006

Images

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN400812) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN400812) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody
Immunohistochemistry (IHC) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN400812) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody (Image 2)
Immunofluorescence (IF) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN400812) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody (Image 3)