anti-Rat (Rattus) STAT5A antibody for Immunofluorescence

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5A-like
  • signal transducer and activator of transcription 5B
  • STAT5A
  • Stat5a
  • LOC100348518
  • STAT5B
Epitope
AA 451-649
102
56
47
22
19
16
10
8
6
6
5
5
5
5
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
493
230
198
35
24
19
17
16
7
4
4
1
1
1
1
1
1
Host
Mouse
359
140
14
Clonality
Monoclonal
Conjugate
This STAT5A antibody is un-conjugated
13
13
11
9
8
8
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
397
233
163
96
88
86
46
42
26
17
11
9
7
7
6
4
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN967810
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.176395 ABIN967811 IF IP IHC WB Mouse IgG2b AA 451-649 Log in to see 89-Stat5 5
11.176395 ABIN2707112 IC IF WB Rabbit Center Log in to see Polyclonal
11.176395 ABIN4356401 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN5550843 EIA IF IHC (p) WB Mouse IgG1 Tyr694 Log in to see 5F6-F1
1 ABIN3187089 ELISA IF WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1104496 ELISA IF IHC (p) WB Mouse IgG pTyr694 Log in to see 5F6-F1
1 ABIN272189 IF WB Rabbit Log in to see Polyclonal
1 ABIN223192 IF WB Rabbit IgG pTyr694 Log in to see Polyclonal
1 ABIN2892110 IF WB Rabbit pSer420 Log in to see Polyclonal
1 ABIN1960083 FACS IF IHC (p) WB Mouse IgG1 Log in to see 9F7
1 ABIN2964322 FACS IF IHC (p) WB Mouse IgG1 full length Log in to see 9F7
1 ABIN4356399 ELISA ICC IF IHC IHC (p) WB Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356410 ELISA ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356411 ELISA ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356412 ELISA ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356421 ELISA ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356420 ELISA ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356423 ELISA ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356422 ELISA ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1
1 ABIN4356405 ELISA ICC IF IHC IHC (p) WB PerCP Mouse IgG1 kappa pTyr694 Log in to see 5F6-F1

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope AA 451-649
(102), (56), (47), (22), (19), (16), (10), (8), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(493), (230), (198), (35), (24), (19), (17), (16), (7), (4), (4), (1), (1), (1), (1), (1), (1)
Host Mouse
(359), (140), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is un-conjugated
(13), (13), (11), (9), (8), (8), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(397), (233), (163), (96), (88), (86), (46), (42), (26), (17), (11), (9), (7), (7), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A antibody (ABIN967810) Images
Cross-Reactivity Human, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Sheep Stat5 aa. 451-649
Clone 89-Stat5
Isotype IgG2b

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A antibody (ABIN967810) Images back to top
Antigen
Alternative Name Stat5 (STAT5A Antibody Abstract)
Background Stat5, also known as mammary gland factor (MGF) has been shown to play a critical role in the lactogenic hormone response and has been well characterized in mammary epithelial cells. The SH3 domain of MGF shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology has led to its classification as a member of the Stat family. The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment and activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation.
Synonyms: MGF
Molecular Weight 92 kDa
Research Area Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A antibody (ABIN967810) Images back to top
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A antibody (ABIN967810) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-STAT5A antibody (ABIN967810)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Product cited in:

Buitenhuis, Baltus, Lammers, Coffer, Koenderman: "Signal transducer and activator of transcription 5a (STAT5a) is required for eosinophil differentiation of human cord blood-derived CD34+ cells." in: Blood, Vol. 101, Issue 1, pp. 134-42, 2002

Chughtai, Schimchowitsch, Lebrun, Ali: "Prolactin induces SHP-2 association with Stat5, nuclear translocation, and binding to the beta-casein gene promoter in mammary cells." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31107-14, 2002

Sadowski, Choi, Le, Wheeler, Wang, Sadowski: "Insulin Induction of SOCS-2 and SOCS-3 mRNA expression in C2C12 Skeletal Muscle Cells Is Mediated by Stat5*." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20703-10, 2001

Kirken, Malabarba, Xu, Liu, Farrar, Hennighausen, Larner, Grimley, Rui: "Prolactin stimulates serine/tyrosine phosphorylation and formation of heterocomplexes of multiple Stat5 isoforms in Nb2 lymphocytes." in: The Journal of biological chemistry, Vol. 272, Issue 22, pp. 14098-103, 1997

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994

Images

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Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (ABIN967810) Western blot analysis of Stat5 on human endothelial cell lysate. Lane 1: 1:250, lane ...
Immunofluorescence (IF) image for anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (ABIN967810) Immunofluorescent staining of rabbit muscle with anti-Stat5 antibody.