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anti-Human STAT5B antibody for Enzyme Immunoassay

Recommended STAT5B Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5B (STAT5B) Antibodies
  • STAT5
  • stat5
  • Stat5
  • STAT5A
  • stat5b
Epitope
C-Term
35
21
12
8
6
5
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
182
82
70
8
8
8
6
4
3
3
Host
Rabbit
134
60
Clonality
Polyclonal
Conjugate
This STAT5B antibody is un-conjugated
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
149
70
62
33
29
22
14
7
6
3
2
1
1
1
1
Supplier
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Catalog No. ABIN357079
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN781828 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D6
0.0008867729 ABIN781829 EIA IF IHC (p) WB Mouse IgG1 Log in to see 2D1
0.0008867729 ABIN452649 EIA Mouse IgG1 pThr694 Log in to see A4B9
0.0008867729 ABIN452651 EIA Mouse IgG2b pThr694 Log in to see A2H6
0.0008867729 ABIN1109153 EIA WB Mouse IgG1 Log in to see 5B3
0.0008867729 ABIN2732826 EIA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-STAT5B antibody for Enzyme Immunoassay

Similar anti-STAT5B Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 64 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 61 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 139 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 5B (STAT5B) Antibodies
Epitope C-Term
(35), (21), (12), (8), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(182), (82), (70), (8), (8), (8), (6), (4), (3), (3)
Host Rabbit
(134), (60)
Clonality
Polyclonal
Conjugate This STAT5B antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(149), (70), (62), (33), (29), (22), (14), (7), (6), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-STAT5B Antibody

Target Details STAT5B Application Details Handling References for anti-STAT5B Antibody (ABIN357079) Images
Specificity This antibody detects STAT5b at C-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human STAT5b.
Isotype Ig

Target Details STAT5B

Product Details anti-STAT5B Antibody Application Details Handling References for anti-STAT5B Antibody (ABIN357079) Images back to top
Antigen
Alternative Name STAT5B (STAT5B Antibody Abstract)
Background STAT5b is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.Synonyms: STAT-5B, Signal transducer and activator of transcription 5B
Molecular Weight 89865 Da
Gene ID 6777, 9606
UniProt P51692
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-STAT5B Antibody Target Details STAT5B Handling References for anti-STAT5B Antibody (ABIN357079) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100,
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT5B Antibody Target Details STAT5B Application Details References for anti-STAT5B Antibody (ABIN357079) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-STAT5B Antibody (ABIN357079)

Product Details anti-STAT5B Antibody Target Details STAT5B Application Details Handling Images back to top
Background publications

Xi, Zhang, Gooding, Smithgall, Grandis: "Constitutive activation of Stat5b contributes to carcinogenesis in vivo." in: Cancer research, Vol. 63, Issue 20, pp. 6763-71, 2003

Uddin, Lekmine, Sassano, Rui, Fish, Platanias: "Role of Stat5 in type I interferon-signaling and transcriptional regulation." in: Biochemical and biophysical research communications, Vol. 308, Issue 2, pp. 325-30, 2003

Zhang, Msc, Moilanen, Lahti, Hämäläinen, Giembycz, Barnes, Lindsay, Kankaanranta: "Regulation of eosinophil apoptosis by nitric oxide: Role of c-Jun-N-terminal kinase and signal transducer and activator of transcription 5." in: The Journal of allergy and clinical immunology, Vol. 112, Issue 1, pp. 93-101, 2003

Yamashita, Iwase, Toyama, Fujii: "Naturally occurring dominant-negative Stat5 suppresses transcriptional activity of estrogen receptors and induces apoptosis in T47D breast cancer cells." in: Oncogene, Vol. 22, Issue 11, pp. 1638-52, 2003

Kloth, Laughlin, Biscardi, Boerner, Parsons, Silva: "STAT5b, a Mediator of Synergism between c-Src and the Epidermal Growth Factor Receptor." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 1671-9, 2003

Images

Product Details anti-STAT5B Antibody Target Details STAT5B Application Details Handling References for anti-STAT5B Antibody (ABIN357079) back to top
Supplier Images
 image for anti-STAT5B antibody (Signal Transducer and Activator of Transcription 5B) (C-Term) (ABIN357079) anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (C-Term) antibody
 image for anti-STAT5B antibody (Signal Transducer and Activator of Transcription 5B) (C-Term) (ABIN357079) anti-Signal Transducer and Activator of Transcription 5B (STAT5B) (C-Term) antibody (Image 2)