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anti-Mouse (Murine) ARAF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • 1200013E08Rik
  • A-RAF
  • a-raf
  • A-Raf
  • araf
  • ARAF
  • ARAF1
  • araf1
  • Araf1
  • AW495444
  • pks2
  • PKS2
  • rafa1
  • RAFA1
  • wu:fk45h07
  • zgc:92074
Reactivity
Human, Mouse (Murine), Rat (Rattus)
129
66
61
3
3
2
2
1
1
Host
Rabbit
108
23
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
86
52
33
29
19
11
5
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN271994
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089850504 ABIN2855039 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089850504 ABIN746078 IF (p) IHC (p) WB Rabbit IgG pTyr301, pTyr302 Log in to see Polyclonal
0.00089850504 ABIN745328 IF (p) IHC (p) WB Rabbit IgG pSer299 Log in to see Polyclonal
0.00089850504 ABIN746080 IHC (p) WB Biotin Rabbit IgG pTyr301, pTyr302 Log in to see Polyclonal
0.00089850504 ABIN746087 IHC (p) WB HRP Rabbit IgG pTyr301, pTyr302 Log in to see Polyclonal
0.00089850504 ABIN745330 IHC (p) WB Biotin Rabbit IgG pSer299 Log in to see Polyclonal
0.00089850504 ABIN745337 IHC (p) WB HRP Rabbit IgG pSer299 Log in to see Polyclonal
0.00089850504 ABIN682770 IHC (p) WB Biotin Rabbit IgG pSer214 Log in to see Polyclonal
0.00089850504 ABIN682777 IHC (p) WB HRP Rabbit IgG pSer214 Log in to see Polyclonal
0.00089850504 ABIN682768 IF (p) IHC (p) WB Rabbit IgG pSer214 Log in to see Polyclonal

Similar anti-ARAF Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 88 Antibodies 32 Antibodies 28 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 3 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 11 Antibodies 10 Antibodies
Immunohistochemistry (IHC) 31 Antibodies 14 Antibodies 13 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies 33 Antibodies
Immunofluorescence (IF) 12 Antibodies

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(129), (66), (61), (3), (3), (2), (2), (1), (1)
Host Rabbit
(108), (23)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(86), (52), (33), (29), (19), (11), (5), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling Images
Specificity This antibody detects endogenous levels of A-RAF protein. (region surrounding Asp297)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name ARAF (ARAF Antibody Abstract)
Background Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks
Molecular Weight approx. 68 kDa
Gene ID 369
NCBI Accession NP_001645
UniProt P10398
Pathways MAPK Signaling, RTK Signaling

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling Images back to top
Application Notes ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling back to top
Supplier Images
 image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (ABIN271994) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibody
 image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (ABIN271994) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibody (Image 2)