anti-Rat (Rattus) ARAF antibody for Immunofluorescence

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • v-raf murine sarcoma 3611 viral oncogene homolog
  • araf
  • ARAF
  • LOC100340437
  • araf-a
  • araf-b
  • Araf
Epitope
AA 276-325, pTyr302
22
20
16
15
14
12
10
9
8
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
146
81
74
6
6
6
3
2
2
2
1
1
Host
Rabbit
120
26
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
98
51
39
29
28
13
6
3
2
2
1
1
1
Options
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Catalog No. ABIN1531487
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967727 IF IP IHC WB Mouse IgG1 AA 183-199 Log in to see 1-A 5
1 ABIN3182781 ELISA IF IHC WB Rabbit IgG pTyr302 Log in to see Polyclonal
1 ABIN2883484 ELISA IF IHC (p) WB Rabbit IgG pTyr302 Log in to see Polyclonal
1 ABIN1574357 IF IHC ELISA WB Rabbit IgG pTyr302 Log in to see Polyclonal
1 ABIN2133975 IF IHC ELISA WB Rabbit IgG pTyr301 Log in to see Polyclonal

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope AA 276-325, pTyr302
(22), (20), (16), (15), (14), (12), (10), (9), (8), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(146), (81), (74), (6), (6), (6), (3), (2), (2), (2), (1), (1)
Host Rabbit
(120), (26)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(98), (51), (39), (29), (28), (13), (6), (3), (2), (2), (1), (1), (1)
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling Images
Specificity

A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A-RAF only when phosphorylated at Tyr302.

PhosphorylationH:Y302 M:Y300 R:Y300

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302.
Isotype IgG

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name A-RAF (ARAF Antibody Abstract)
Background

Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2

NCBI Gene Symbol: ARAF

Molecular Weight 67 kDa
Gene ID 369
OMIM 311010
UniProt P10398
Pathways MAPK Signaling, RTK Signaling, Hepatitis C

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
Comment

Unigene-Number: Hs.225936, Hs.446641 (NCBI Gene Symbol: ARAF)

Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 276-325), (pTyr302) antibody (ABIN1531487) Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', usin...
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 276-325), (pTyr302) antibody (ABIN1531487) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RA...
Immunofluorescence (IF) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 276-325), (pTyr302) antibody (ABIN1531487) Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. Th...