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anti-Human BAD antibody for Dot Blot

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • AI325008
  • bad
  • BAD
  • Bbc2
  • BBC2
  • BCL2L8
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
Epitope
pSer99
51
47
41
38
36
36
34
27
27
24
20
20
20
20
20
20
20
19
19
19
19
19
19
19
19
19
19
19
18
17
16
16
15
13
12
10
9
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
726
377
217
9
5
4
2
2
1
1
Host
Rabbit
919
50
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
66
65
65
59
59
59
6
6
6
6
6
6
6
6
6
Application
Dot Blot (DB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
511
433
417
293
74
67
54
26
10
8
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN389521
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.63784635 ABIN1539690 DB Rabbit Ig pSer1159 Log in to see Polyclonal 1
0.63784635 ABIN1539741 DB Rabbit Ig pTyr158 Log in to see Polyclonal 1
0.63784635 ABIN1881101 DB Rabbit pSer57 Log in to see Polyclonal 2
0.63784635 ABIN1881098 DB Rabbit pSer124 Log in to see Polyclonal 2
0.63784635 ABIN1881099 DB Rabbit pSer32 Log in to see Polyclonal 2
0.63784635 ABIN1881100 DB Rabbit pThr80 Log in to see Polyclonal 2
0.63784635 ABIN1539706 DB Rabbit Ig AA 41-70, pSer64 Log in to see Polyclonal
0.63784635 ABIN1539776 DB Rabbit Ig pSer74 Log in to see Polyclonal
0.63784635 ABIN1539773 DB Rabbit Ig pSer71 Log in to see Polyclonal
0.63784635 ABIN1539737 DB Rabbit Ig pTyr110 Log in to see Polyclonal
0.63784635 ABIN1539722 DB Rabbit Ig pThr137 Log in to see Polyclonal
0.63784635 ABIN1539757 DB Rabbit Ig pSer1041 Log in to see Polyclonal
0.63784635 ABIN1539698 DB Rabbit Ig pSer25 Log in to see Polyclonal
0.63784635 ABIN1539717 DB Rabbit Ig pSer91 Log in to see Polyclonal
0.63784635 ABIN1539752 DB Rabbit Ig pTyr76 Log in to see Polyclonal
0.63784635 ABIN2159427 DB Rabbit Ig Fraction pSer34 Log in to see Polyclonal
0.63784635 ABIN2159426 DB Rabbit Ig Fraction pSer25 Log in to see Polyclonal
0.63784635 ABIN2159430 DB Rabbit Ig Fraction pSer91 Log in to see Polyclonal
0.63784635 ABIN2159428 DB Rabbit Ig Fraction pSer71 Log in to see Polyclonal
0.63784635 ABIN2159431 DB Rabbit Ig Fraction pThr137 Log in to see Polyclonal

Top referenced anti-BAD antibody for Dot Blot

  • Human Polyclonal BAD Primary Antibody for DB - ABIN1881101 (2 Publications): Galmiche, Ezzoukhry, François, Louandre, Sabbagh, Nguyen-Khac, Descamps, Trouillet, Godin, Regimbeau, Joly, Barbare, Duverlie, Mazière, Chatelain: BAD, a proapoptotic member of the BCL2 family, is a potential therapeutic target in hepatocellular carcinoma. in Molecular cancer research : MCR 2010 (PubMed)

  • Human Polyclonal BAD Primary Antibody for DB - ABIN1881098 (2 Publications): Yu, Sun, Shen, Gao, Fan, Sun: Expression of the apoptosis-related genes BCL-2 and BAD in human breast carcinoma and their associated relationship with chemosensitivity. in Journal of experimental & clinical cancer research : CR 2010 (PubMed)

  • Human Polyclonal BAD Primary Antibody for DB - ABIN1539690 (1 Publications): Liu, Graves, Scott, Li, Lin: Akt switches TopBP1 function from checkpoint activation to transcriptional regulation through phosphoserine binding-mediated oligomerization. in Molecular and cellular biology 2013 (PubMed)

  • Human Polyclonal BAD Primary Antibody for DB - ABIN1539741 (1 Publications): Almeida, Mesquita, Cruz, Osório, Custódio, Brito, Vingadassalom, Martins, Leong, Holden, Cabanes, Sousa: Src-dependent tyrosine phosphorylation of non-muscle myosin heavy chain-IIA restricts Listeria monocytogenes cellular infection. in The Journal of biological chemistry 2015 (PubMed)

Similar anti-BAD Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 250 Antibodies
ELISA 365 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 276 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 65 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Immunostaining (ISt) 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 381 Antibodies

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope pSer99
(51), (47), (41), (38), (36), (36), (34), (27), (27), (24), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (17), (16), (16), (15), (13), (12), (10), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(726), (377), (217), (9), (5), (4), (2), (2), (1), (1)
Host Rabbit
(919), (50), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BAD antibody is un-conjugated
(66), (65), (65), (59), (59), (59), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Dot Blot (DB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(511), (433), (417), (293), (74), (67), (54), (26), (10), (8), (4), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN389521) Images
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This Bad Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
Clone RB06933
Isotype Ig

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling References for anti-BAD Antibody (ABIN389521) Images back to top
Antigen
Alternative Name Bad (BAD Antibody Abstract)
Background Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

Synonyms: Bcl2-antagonist of cell death, BAD, Bcl-2 binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8,
Pathways: Neurotrophin signaling pathway, Hepatitis C, Insulin signaling pathway, Alzheimer's disease, VEGF signaling pathway, Toxoplasmosis, Pathways in cancer, Non-small cell lung cancer, Tuberculosis, Focal adhesion, Prostate cancer, Melanoma, Apoptosis, Colorectal cancer, Pancreatic cancer, ErbB signaling pathway, Chronic myeloid leukemia, Endometrial cancer, Acute myeloid leukemia, Amyotrophic lateral sclerosis (ALS)
Molecular Weight 18392 Da
Gene ID 572
UniProt Q92934
Research Area Cancer, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling References for anti-BAD Antibody (ABIN389521) Images back to top
Application Notes Suggested dilutions:
IF = 1:200, DB = 1:500, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details References for anti-BAD Antibody (ABIN389521) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-BAD Antibody (ABIN389521)

Product Details anti-BAD Antibody Target Details BAD Application Details Handling Images back to top
Background publications

Hurbin, Coll, Dubrez-Daloz, Mari, Auberger, Brambilla, Favrot: "Cooperation of amphiregulin and insulin-like growth factor-1 inhibits Bax- and Bad-mediated apoptosis via a protein kinase C-dependent pathway in non-small cell lung cancer cells." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19757-67, 2005

Antignani, Youle: "A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad." in: Biochemistry, Vol. 44, Issue 10, pp. 4074-82, 2005

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN389521) back to top
Supplier Images
Dot Blot (DB) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (pSer99) (ABIN389521) Dot blot analysis of anti-hBad-pS99 Phospho-specific Pab (ABIN389521) on nitrocellulo...
Immunohistochemistry (IHC) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (pSer99) (ABIN389521) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...