anti-Human BAD antibody for Dot Blot

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-antagonist of cell death b
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitope
pSer99
60
58
52
49
46
45
43
29
22
21
21
20
20
20
20
20
20
20
20
20
20
20
20
20
20
19
19
19
19
15
15
12
10
9
8
8
7
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
779
435
265
19
15
8
5
4
3
2
2
1
1
1
1
1
1
Host
Rabbit
957
49
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
65
64
64
58
58
58
6
6
6
6
6
6
6
6
6
6
6
Application
Dot Blot (DB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
527
465
395
295
113
79
53
28
13
12
6
5
4
3
2
1
1
1
Options
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Catalog No. ABIN389521
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.667469 ABIN1539741 DB Rabbit Ig pTyr158 Log in to see Polyclonal 1
28.667469 ABIN1539690 DB Rabbit Ig pSer1159 Log in to see Polyclonal 1
28.667469 ABIN1881099 DB Rabbit pSer32 Log in to see Polyclonal 2
28.667469 ABIN1881100 DB Rabbit pThr80 Log in to see Polyclonal 2
28.667469 ABIN1881101 DB Rabbit pSer57 Log in to see Polyclonal 2
28.667469 ABIN1881098 DB Rabbit pSer124 Log in to see Polyclonal 2
28.667469 ABIN1539722 DB Rabbit Ig pThr137 Log in to see Polyclonal
28.667469 ABIN1539706 DB Rabbit Ig AA 41-70, pSer64 Log in to see Polyclonal
28.667469 ABIN1539776 DB Rabbit Ig pSer74 Log in to see Polyclonal
28.667469 ABIN1539737 DB Rabbit Ig pTyr110 Log in to see Polyclonal
28.667469 ABIN1539773 DB Rabbit Ig pSer71 Log in to see Polyclonal
28.667469 ABIN1539698 DB Rabbit Ig pSer25 Log in to see Polyclonal
28.667469 ABIN1539757 DB Rabbit Ig pSer1041 Log in to see Polyclonal
28.667469 ABIN1539752 DB Rabbit Ig pTyr76 Log in to see Polyclonal
28.667469 ABIN1539717 DB Rabbit Ig pSer91 Log in to see Polyclonal
28.667469 ABIN3030133 DB ELISA Rabbit Ig Fraction pTyr110 Log in to see Polyclonal
28.667469 ABIN3030130 DB IF IHC ELISA Rabbit Ig Fraction pSer99 Log in to see Polyclonal
28.667469 ABIN3030131 DB ELISA Rabbit Ig Fraction pThr137 Log in to see Polyclonal
28.667469 ABIN3030119 DB ELISA Rabbit Ig Fraction pSer124 Log in to see Polyclonal
28.667469 ABIN3030123 DB ELISA Rabbit Ig Fraction pSer322 Log in to see Polyclonal

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope pSer99
(60), (58), (52), (49), (46), (45), (43), (29), (22), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (15), (15), (12), (10), (9), (8), (8), (7), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(779), (435), (265), (19), (15), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(957), (49), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BAD antibody is un-conjugated
(65), (64), (64), (58), (58), (58), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Dot Blot (DB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(527), (465), (395), (295), (113), (79), (53), (28), (13), (12), (6), (5), (4), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling ProductDetails: References for anti-BAD antibody (ABIN389521) Images
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This Bad Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
Clone RB06933
Isotype Ig

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling ProductDetails: References for anti-BAD antibody (ABIN389521) Images back to top
Antigen
Alternative Name Bad (BAD Antibody Abstract)
Background Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

Synonyms: Bcl2-antagonist of cell death, BAD, Bcl-2 binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8,
Pathways: Neurotrophin signaling pathway, Hepatitis C, Insulin signaling pathway, Alzheimer's disease, VEGF signaling pathway, Toxoplasmosis, Pathways in cancer, Non-small cell lung cancer, Tuberculosis, Focal adhesion, Prostate cancer, Melanoma, Apoptosis, Colorectal cancer, Pancreatic cancer, ErbB signaling pathway, Chronic myeloid leukemia, Endometrial cancer, Acute myeloid leukemia, Amyotrophic lateral sclerosis (ALS)
Molecular Weight 18392 Da
Gene ID 572
UniProt Q92934
Research Area Cancer, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling ProductDetails: References for anti-BAD antibody (ABIN389521) Images back to top
Application Notes Suggested dilutions:
IF = 1:200, DB = 1:500, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details ProductDetails: References for anti-BAD antibody (ABIN389521) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

ProductDetails: References for anti-BAD antibody (ABIN389521)

Product Details anti-BAD Antibody Target Details BAD Application Details Handling Images back to top
Background publications

Hurbin, Coll, Dubrez-Daloz, Mari, Auberger, Brambilla, Favrot: "Cooperation of amphiregulin and insulin-like growth factor-1 inhibits Bax- and Bad-mediated apoptosis via a protein kinase C-dependent pathway in non-small cell lung cancer cells." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19757-67, 2005

Antignani, Youle: "A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad." in: Biochemistry, Vol. 44, Issue 10, pp. 4074-82, 2005

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling ProductDetails: References for anti-BAD antibody (ABIN389521) back to top
Supplier Images
Dot Blot (DB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody (ABIN389521) Dot blot analysis of anti-hBad-pS99 Phospho-specific Pab (ABIN389521) on nitrocellulo...
Immunohistochemistry (IHC) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody (ABIN389521) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...