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anti-Human BAD antibody for Immunofluorescence

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • AI325008
  • bad
  • BAD
  • Bbc2
  • BBC2
  • BCL2L8
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
Epitope
pSer134
50
47
41
37
37
36
34
27
27
24
20
20
20
20
20
20
20
19
19
19
19
19
19
19
19
19
19
19
18
17
16
16
15
13
12
10
9
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
724
374
215
9
5
4
2
2
1
1
Host
Rabbit
918
49
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
66
65
65
59
59
59
6
6
6
6
6
6
6
6
6
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
509
430
418
291
74
67
53
26
10
8
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN1531524
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32106254 ABIN967941 IF IP IHC WB Mouse IgG2b AA 39-198 Log in to see 48-Bad 5
0.32106254 ABIN967940 IF IP IHC WB Mouse IgG2b AA 39-198 Log in to see 48-Bad 5
0.32106254 ABIN2704517 IC IF IHC WB Rabbit C-Term, pSer155 Log in to see Polyclonal
0.32106254 ABIN2162905 IF IHC ELISA WB Rabbit IgG Ser134 Log in to see Polyclonal
0.32106254 ABIN2159438 DB IF Rabbit Ig Fraction pSer99 Log in to see Polyclonal
0.0010843406 ABIN389521 DB IF IHC (p) Rabbit Ig pSer99 Log in to see Polyclonal 2
0.0010843406 ABIN268326 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y208
0.0010843406 ABIN446783 ICC IF IHC (p) IHC WB Rabbit AA 110-160 Log in to see Polyclonal
0.0010843406 ABIN2891940 IF IHC WB Rabbit Pro130 Log in to see Polyclonal
0.0010843406 ABIN3183465 ELISA IF IHC WB Rabbit IgG Ser163 Log in to see Polyclonal
0.0010843406 ABIN3182608 ELISA IF IHC WB Rabbit IgG pSer91 Log in to see Polyclonal
0.0010843406 ABIN3181929 ELISA IF IHC WB Rabbit IgG pSer155 Log in to see Polyclonal
0.0010843406 ABIN3182787 ELISA IF IHC WB Rabbit IgG pSer134 Log in to see Polyclonal
0.0010843406 ABIN1976926 IF IHC (p) Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0010843406 ABIN1976925 IF IP IHC (p) Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0010843406 ABIN1840429 DB IF IHC (p) WB Rabbit pSer99 Log in to see Polyclonal
0.0010843406 ABIN2208139 IC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010843406 ABIN4282853 ICC IF IHC IHC (p) WB Rabbit pSer155 Log in to see Polyclonal
0.0010843406 ABIN2959614 IF IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010843406 ABIN3030130 DB IF IHC ELISA Rabbit Ig Fraction pSer99 Log in to see Polyclonal

Top referenced anti-BAD antibody for Immunofluorescence

Similar anti-BAD Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 36 Antibodies 132 Antibodies
ELISA 81 Antibodies 164 Antibodies
Flow Cytometry (FACS) 3 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 15 Antibodies 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 122 Antibodies 144 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Western Blotting (WB) 131 Antibodies

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope pSer134
(50), (47), (41), (37), (37), (36), (34), (27), (27), (24), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (17), (16), (16), (15), (13), (12), (10), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(724), (374), (215), (9), (5), (4), (2), (2), (1), (1)
Host Rabbit
(918), (49), (2), (1)
Clonality
Polyclonal
Conjugate This BAD antibody is un-conjugated
(66), (65), (65), (59), (59), (59), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(509), (430), (418), (291), (74), (67), (53), (26), (10), (8), (4), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN1531524) Images
Specificity BAD (Phospho-Ser134) Antibody detects endogenous levels of BAD only when phosphorylated at Ser134.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human BAD around the phosphorylation site of Ser134.
Isotype IgG

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling References for anti-BAD Antibody (ABIN1531524) Images back to top
Antigen
Alternative Name BAD (BAD Antibody Abstract)
Background Molecular weight of target protein: 22 kDa
Synonyms: BAD, BBC6, Bcl- XL/Bcl-2 associated death promoter, Bcl-2 binding component 6, Bcl-2-like 8 protein, Bcl-xL/Bcl-2 associated death promoter, Bcl2-antagonist of cell death, BCL2L8
Molecular Weight 22 kDa
Gene ID 572
OMIM 603167
UniProt Q92934
Research Area Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling References for anti-BAD Antibody (ABIN1531524) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.370254 (NCBI Gene Symbol: BAD)

Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details References for anti-BAD Antibody (ABIN1531524) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-BAD Antibody (ABIN1531524)

Product Details anti-BAD Antibody Target Details BAD Application Details Handling Images back to top
Background publications

Wang, Rapp, Reed: "Bcl-2 targets the protein kinase Raf-1 to mitochondria." in: Cell, Vol. 87, Issue 4, pp. 629-38, 1997

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN1531524) back to top
Supplier Images
Western Blotting (WB) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (pSer134) (ABIN1531524) Western blot analysis of extracts from mouse liver, using BAD (Phospho-Ser134) Antibo...
Immunohistochemistry (IHC) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (pSer134) (ABIN1531524) Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Se...
Immunofluorescence (IF) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (pSer134) (ABIN1531524) Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) Antibody. The p...