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anti-Human BAD antibody for Immunoprecipitation

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • AI325008
  • bad
  • BAD
  • Bbc2
  • BBC2
  • BCL2L8
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
Epitope
AA 39-198
51
45
41
37
36
36
34
27
27
23
20
20
20
20
20
20
20
19
19
19
19
19
19
19
19
19
19
19
18
17
16
16
15
13
12
10
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
710
364
207
9
5
4
2
2
1
Host
Mouse
904
47
2
1
Clonality
Monoclonal
Conjugate
This BAD antibody is un-conjugated
66
65
65
59
59
59
6
6
6
6
6
6
6
6
6
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
509
418
415
278
67
60
45
22
8
4
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN967941
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089396554 ABIN967940 IF IP IHC WB Mouse IgG2b AA 39-198 Log in to see 48-Bad 5
0.00089396554 ABIN268326 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y208
0.00089396554 ABIN234359 IP ELISA WB Mouse IgG1 Log in to see 5E6
0.00089396554 ABIN2469925 IP IHC WB Mouse IgG N-Term Log in to see BYC001
0.00089396554 ABIN957139 IHC (fro) FACS IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089396554 ABIN222885 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089396554 ABIN223026 IP IHC WB Rabbit IgG pSer112 Log in to see Polyclonal
0.00089396554 ABIN408791 IP ELISA WB Mouse IgG1 Ser155 Log in to see 27AT937
0.00089396554 ABIN957137 IHC (fro) IHC (p) IP WB Rabbit AA 87-103 Log in to see Polyclonal
0.00089396554 ABIN1723248 IHC (p) IP WB Mouse IgG N-Term, His6 Log in to see BYC001
0.00089396554 ABIN408803 IP ELISA WB Mouse IgG1 Ser155 Log in to see 27AT381
0.00089396554 ABIN1967878 IP IHC WB Rabbit Log in to see Polyclonal
0.00089396554 ABIN1584052 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.00089396554 ABIN2736342 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089396554 ABIN1976925 IF IP IHC (p) Rabbit IgG AA 1-50 Log in to see Polyclonal
0.00089396554 ABIN2208128 IP IHC WB Rabbit IgG AA 87-103 Log in to see Polyclonal
0.00089396554 ABIN1001857 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089396554 ABIN2208164 IP WB Mouse IgG1 Log in to see 9L48
0.00089396554 ABIN1003499 IP IHC WB Rabbit phosphospecific Log in to see Polyclonal
0.00089396554 ABIN2208104 IP WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 9L48

Top referenced anti-BAD antibody for Immunoprecipitation

Similar anti-BAD Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 36 Antibodies 132 Antibodies
ELISA 80 Antibodies 163 Antibodies
Flow Cytometry (FACS) 3 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 14 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 114 Antibodies 135 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 123 Antibodies

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope AA 39-198
(51), (45), (41), (37), (36), (36), (34), (27), (27), (23), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (17), (16), (16), (15), (13), (12), (10), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(710), (364), (207), (9), (5), (4), (2), (2), (1)
Host Mouse
(904), (47), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAD antibody is un-conjugated
(66), (65), (65), (59), (59), (59), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(509), (418), (415), (278), (67), (60), (45), (22), (8), (4), (4), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN967941) Images
Cross-Reactivity Human, Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse Bad aa. 39-198
Clone 48-Bad
Isotype IgG2b

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling References for anti-BAD Antibody (ABIN967941) Images back to top
Antigen
Alternative Name Bad (BAD Antibody Abstract)
Background Isolated by screening for Bcl-2 interacting proteins, Bad shows significant homology to Bcl-2 within the Bcl-2 homology domains 1 and 2 (BH1 and BH2). In addition, several other proteins involved in cell death such as Bax, Bcl-X[L], Mcl-1, and A1 share similar homology with Bcl-2. Bcl-2 is known to oppose several apoptotic signals and is considered to be a central downstream cell death repressor. Bcl-X[L] represses apoptosis, but its short form, Bcl-X[S], promotes cell death. Bax is known to homodimerize as well as heterodimerize with Bcl-2. An excess concentration of Bax opposes the ability of Bcl-2 to repress cell death. Bad can selectively dimerize with Bcl-X[L] and Bcl-2, but not with Bax, Bcl-X[S], Mcl-1, A1, or itself. In mammalian cells, Bad binds more strongly to Bcl-X[L] than Bcl-2. This may explain why Bad reverses the death repressor activity of Bcl-X[L], but not that of Bcl-2. The formation of the Bad-Bcl-X[L] heterodimer displaces Bax and restores favorable conditions for apoptosis. This antibody is tested by western blot analysis.
Molecular Weight 23 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling References for anti-BAD Antibody (ABIN967941) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details References for anti-BAD Antibody (ABIN967941) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-BAD Antibody (ABIN967941)

Product Details anti-BAD Antibody Target Details BAD Application Details Handling Images back to top
Product cited in:

Tomicic, Thust, Kaina: "Ganciclovir-induced apoptosis in HSV-1 thymidine kinase expressing cells: critical role of DNA breaks, Bcl-2 decline and caspase-9 activation." in: Oncogene, Vol. 21, Issue 14, pp. 2141-53, 2002

Walsh, Lutz, Cotter, OConnor: "Erythrocyte survival is promoted by plasma and suppressed by a Bak-derived BH3 peptide that interacts with membrane-associated Bcl-X(L)." in: Blood, Vol. 99, Issue 9, pp. 3439-48, 2002

Ayllón, Cayla, García, Roncal, Fernández, Albar, Martínez, Rebollo: "Bcl-2 targets protein phosphatase 1 alpha to Bad." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7345-52, 2001

Graff, Konicek, McNulty, Wang, Houck, Allen, Paul, Hbaiu, Goode, Sandusky, Vessella, Neubauer: "Increased AKT activity contributes to prostate cancer progression by dramatically accelerating prostate tumor growth and diminishing p27Kip1 expression." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24500-5, 2000

Yang, Zha, Jockel, Boise, Thompson, Korsmeyer: "Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death." in: Cell, Vol. 80, Issue 2, pp. 285-91, 1995

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling References for anti-BAD Antibody (ABIN967941) back to top
Supplier Images
Western Blotting (WB) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 39-198) (ABIN967941) Western blot analysis of Bad on an A431 cell lysate (Human epithelial carcinoma, ATCC...
Immunofluorescence (IF) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 39-198) (ABIN967941) Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).
 image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (AA 39-198) (ABIN967941) anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 39-198) antibody (Image 3)