anti-Human BAD antibody for Immunoprecipitation

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-antagonist of cell death b
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitope
AA 39-198
60
58
52
49
47
45
43
29
22
21
21
20
20
20
20
20
20
20
20
20
20
20
20
20
20
19
19
19
19
15
15
12
10
9
8
8
7
5
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
779
434
264
19
15
8
5
4
3
2
2
1
1
1
1
1
1
Host
Mouse
958
48
2
1
Clonality
Monoclonal
Conjugate
This BAD antibody is un-conjugated
65
64
64
58
58
58
6
6
6
6
6
6
6
6
6
6
6
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
527
464
396
294
114
79
52
28
13
12
6
5
4
3
2
1
1
1
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Catalog No. ABIN967940
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967941 IF IP IHC WB Mouse IgG2b AA 39-198 Log in to see 48-Bad 5
1 ABIN461986 IHC (p) IP WB Mouse IgG N-Term Log in to see BYC001
1 ABIN234359 IP ELISA WB Mouse IgG1 Log in to see 5E6
1 ABIN2469925 IP IHC WB Mouse IgG N-Term Log in to see BYC001
1 ABIN4282865 IHC (p) IP WB Mouse IgG N-Term Log in to see BYC001
1 ABIN222885 IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN223026 IP IHC WB Rabbit IgG pSer112 Log in to see Polyclonal
1 ABIN204711 IP ELISA WB Mouse IgG1 pSer112 Log in to see
1 ABIN126732 IF IP WB Mouse IgG1 Log in to see 5-00E-006
1 ABIN957139 IHC (fro) FACS IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN222249 IP ELISA WB Rabbit IgG pSer112 Log in to see Polyclonal
1 ABIN1723248 IHC (p) IP WB Mouse IgG full length Log in to see BYC001
1 ABIN408803 IP ELISA WB Mouse IgG1 pSer155 Log in to see 27AT381
1 ABIN957137 IHC (fro) IHC (p) IP WB Rabbit AA 87-103 Log in to see Polyclonal
1 ABIN126976 IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN241748 IHC IP WB Rabbit pSer112 Log in to see Polyclonal
1 ABIN4282859 ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN1584052 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1821819 IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN127069 IP WB Rabbit pSer112, pSer75 Log in to see Polyclonal

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Epitope AA 39-198
(60), (58), (52), (49), (47), (45), (43), (29), (22), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (15), (15), (12), (10), (9), (8), (8), (7), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(779), (434), (264), (19), (15), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(958), (48), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAD antibody is un-conjugated
(65), (64), (64), (58), (58), (58), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(527), (464), (396), (294), (114), (79), (52), (28), (13), (12), (6), (5), (4), (3), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling ProductDetails: References for anti-BAD antibody (ABIN967940) Images
Cross-Reactivity Human, Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse Bad aa. 39-198
Clone 48-Bad
Isotype IgG2b

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling ProductDetails: References for anti-BAD antibody (ABIN967940) Images back to top
Antigen
Alternative Name Bad (BAD Antibody Abstract)
Background Isolated by screening for Bcl-2 interacting proteins, Bad shows significant homology to Bcl-2 within the Bcl-2 homology domains 1 and 2 (BH1 and BH2). In addition, several other proteins involved in cell death such as Bax, Bcl-X[L], Mcl-1, and A1 share similar homology with Bcl-2. Bcl-2 is known to oppose several apoptotic signals and is considered to be a central downstream cell death repressor. Bcl-X[L] represses apoptosis, but its short form, Bcl-X[S], promotes cell death. Bax is known to homodimerize as well as heterodimerize with Bcl-2. An excess concentration of Bax opposes the ability of Bcl-2 to repress cell death. Bad can selectively dimerize with Bcl-X[L] and Bcl-2, but not with Bax, Bcl-X[S], Mcl-1, A1, or itself. In mammalian cells, Bad binds more strongly to Bcl-X[L] than Bcl-2. This may explain why Bad reverses the death repressor activity of Bcl-X[L], but not that of Bcl-2. The formation of the Bad-Bcl-X[L] heterodimer displaces Bax and restores favorable conditions for apoptosis. This antibody is tested by western blot analysis.
Molecular Weight 23 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling ProductDetails: References for anti-BAD antibody (ABIN967940) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details ProductDetails: References for anti-BAD antibody (ABIN967940) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

ProductDetails: References for anti-BAD antibody (ABIN967940)

Product Details anti-BAD Antibody Target Details BAD Application Details Handling Images back to top
Product cited in:

Tomicic, Thust, Kaina: "Ganciclovir-induced apoptosis in HSV-1 thymidine kinase expressing cells: critical role of DNA breaks, Bcl-2 decline and caspase-9 activation." in: Oncogene, Vol. 21, Issue 14, pp. 2141-53, 2002

Walsh, Lutz, Cotter, OConnor: "Erythrocyte survival is promoted by plasma and suppressed by a Bak-derived BH3 peptide that interacts with membrane-associated Bcl-X(L)." in: Blood, Vol. 99, Issue 9, pp. 3439-48, 2002

Ayllón, Cayla, García, Roncal, Fernández, Albar, Martínez, Rebollo: "Bcl-2 targets protein phosphatase 1 alpha to Bad." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7345-52, 2001

Graff, Konicek, McNulty, Wang, Houck, Allen, Paul, Hbaiu, Goode, Sandusky, Vessella, Neubauer: "Increased AKT activity contributes to prostate cancer progression by dramatically accelerating prostate tumor growth and diminishing p27Kip1 expression." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24500-5, 2000

Background publications

Yang, Zha, Jockel, Boise, Thompson, Korsmeyer: "Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death." in: Cell, Vol. 80, Issue 2, pp. 285-91, 1995

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling ProductDetails: References for anti-BAD antibody (ABIN967940) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 39-198) antibody (ABIN967940) Western blot analysis of Bad on an A431 cell lysate (Human epithelial carcinoma, ATCC...
Immunofluorescence (IF) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 39-198) antibody (ABIN967940) Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).