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anti-Rat (Rattus) BAD antibody for Immunohistochemistry (Frozen Sections)

Recommended BAD Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antibodies
  • AI325008
  • bad
  • BAD
  • Bbc2
  • BBC2
  • BCL2L8
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
Reactivity
Human, Mouse (Murine), Rat (Rattus)
710
364
207
9
5
4
2
2
1
Host
Rabbit
903
48
2
1
Clonality
Polyclonal
Conjugate
This BAD antibody is un-conjugated
66
65
65
59
59
59
6
6
6
6
6
6
6
6
6
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
509
418
415
279
67
60
46
23
8
4
3
3
2
2
1
1
1
Supplier
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Catalog No. ABIN187916
$ 744.33
Plus shipping costs $45.00

Similar anti-BAD Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Western Blotting (WB) 177 Antibodies 368 Antibodies 123 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 31 Antibodies 32 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies 26 Antibodies 17 Antibodies
Immunohistochemistry (IHC) 265 Antibodies 135 Antibodies 114 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies 34 Antibodies

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(710), (364), (207), (9), (5), (4), (2), (2), (1)
Host Rabbit
(903), (48), (2), (1)
Clonality
Polyclonal
Conjugate This BAD antibody is un-conjugated
(66), (65), (65), (59), (59), (59), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(509), (418), (415), (279), (67), (60), (46), (23), (8), (4), (3), (3), (2), (2), (1), (1), (1)
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Product Details anti-BAD Antibody

Target Details BAD Application Details Handling Images
Specificity Recognizes Bad. Species Reactivity: Human rat and mouse. Other species not tested.
Immunogen Anti-Bad polyclonal antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad (GenBank accession no. Q92934).

Target Details BAD

Product Details anti-BAD Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2-like 8 (BAD Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation, it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.
Research Area Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-BAD Antibody Target Details BAD Handling Images back to top
Application Notes Immunohistochemistry: Use at 2 µg/mL. Western blot: Recommend 0.5-1.0 µg/mL. The optimal dilution for a specific application should be determined by the researcher. Figure 1. Western blot analysis of Bad in T24 cell lysates with anti-Bad at (A) 0.5, (B) 1 and (C) 2 µg/mL. Figure 2. Immunohistochemical staining of rat thymus using anti-Bad at 2 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-BAD Antibody Target Details BAD Application Details Images back to top
Format Liquid
Buffer 100 µg at 1 mg/mL of ion exchange chromatography purified IgG in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

Images

Product Details anti-BAD Antibody Target Details BAD Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (ABIN187916) anti-BCL2-Associated Agonist of Cell Death (BAD) antibody
Western Blotting (WB) image for anti-BAD antibody (BCL2-Associated Agonist of Cell Death) (ABIN187916) anti-BCL2-Associated Agonist of Cell Death (BAD) antibody (Image 2)