anti-Human BRAF antibody for ELISA

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Epitope
AA 346-445, partial
36
33
29
24
22
21
21
18
16
12
11
10
10
9
8
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
406
85
69
13
10
4
4
3
1
1
1
1
Host
Mouse
309
93
3
2
Clonality
Monoclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Proximity Ligation Assay (PLA), ELISA, Western Blotting (WB)
267
209
145
78
71
45
39
22
11
8
5
4
1
1
1
Supplier
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Catalog No. ABIN513800
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.504103 ABIN513799 ELISA WB Mouse IgM kappa AA 346-445, partial Log in to see 3C6
12.504103 ABIN560094 ELISA WB Mouse IgG1 kappa AA 346-445, partial Log in to see 3C6
12.504103 ABIN513801 ELISA WB Mouse IgG2a kappa AA 138-231, partial Log in to see 2E9
12.504103 ABIN513806 ELISA WB Mouse IgG2a kappa AA 138-231, partial Log in to see 3F5
12.504103 ABIN513803 ELISA WB Mouse IgG2b kappa AA 138-231, partial Log in to see 4G7
12.504103 ABIN513804 ELISA WB Mouse IgG2b kappa AA 138-231, partial Log in to see 3A6
12.504103 ABIN513805 ELISA WB Mouse IgG2b kappa AA 138-231, partial Log in to see 1F2
12.504103 ABIN513807 ELISA WB Mouse IgG2a kappa AA 138-231, partial Log in to see 4F4
12.504103 ABIN513809 ELISA WB Mouse IgG2a kappa AA 138-231, partial Log in to see 2F6
12.504103 ABIN513802 IF ELISA WB Mouse IgG2a kappa AA 138-231, partial Log in to see 2F7
12.504103 ABIN2672314 LMNX ELISA Mouse IgG2b Log in to see OTI1G8
12.504103 ABIN2672313 LMNX ELISA Mouse IgG2a Log in to see OTI3A7
12.504103 ABIN2671841 LMNX ELISA Mouse IgG1 Log in to see OTI3C3
12.504103 ABIN2671840 LMNX ELISA Mouse IgG1 Log in to see OTI4C11
1 ABIN2869395 ELISA ICC IHC WB Mouse IgG1 Log in to see 1H12 2
1 ABIN968991 ICC IHC ELISA WB Mouse IgG1 Log in to see 1H12 2
1 ABIN462545 IF IHC (p) ELISA WB Mouse IgG1 AA 285-401 Log in to see 1H12
1 ABIN2869224 ELISA IHC WB Mouse IgG1 Log in to see 1H12F1-1H12G10-1F12F11C9 5
1 ABIN2879057 IF IHC (p) ELISA WB Rabbit IgG AA 171-220 Log in to see Polyclonal
1 ABIN2878975 IHC (p) ELISA WB Rabbit IgG AA 411-460 Log in to see Polyclonal

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 346-445, partial
(36), (33), (29), (24), (22), (21), (21), (18), (16), (12), (11), (10), (10), (9), (8), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(406), (85), (69), (13), (10), (4), (4), (3), (1), (1), (1), (1)
Host Mouse
(309), (93), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Proximity Ligation Assay (PLA), ELISA, Western Blotting (WB)
(267), (209), (145), (78), (71), (45), (39), (22), (11), (8), (5), (4), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN513800) Images
Cross-Reactivity Human
Immunogen BRAF (NP_004324, 346 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: FRPADEDHRN QFGQRDRSSS APNVHINTIE PVNIDDLIRD QGFRGDGGST TGLSATPPAS LPGSLTNVKA LQKSPGPQRE RKSSSSSEDR NRMKTLGRRD
Clone 3D2
Isotype IgG1 kappa

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN513800) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background Full Gene Name: v-raf murine sarcoma viral oncogene homolog B1
Synonyms: B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
Gene ID 673
NCBI Accession NP_004324, NM_004333
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling ProductDetails: References for anti-BRAF antibody (ABIN513800) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Product Quality tested by: Antibody Reactive Against Recombinant Protein.

Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details ProductDetails: References for anti-BRAF antibody (ABIN513800) Images back to top
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

ProductDetails: References for anti-BRAF antibody (ABIN513800)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Liu, Chen, Chau, Jan, Chen, Hsu, Lin, Juang, Lu, Cheng, Chen, Chang, Ting, Kao, Hsiao, Huang: "Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 5, pp. 1335-49, 2013

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN513800) back to top
Supplier Images
ELISA image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 346-445), (partial) antibody (ABIN513800) Detection limit for recombinant GST tagged BRAF is approximately 0.03ng/ml as a captu...
Proximity Ligation Assay (PLA) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 346-445), (partial) antibody (ABIN513800) Proximity Ligation Analysis of protein-protein interactions between MAPK3 and BRAF. H...
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 346-445), (partial) antibody (ABIN513800) BRAF monoclonal antibody (M02), clone 3D2. Western Blot analysis of BRAF expression i...
 image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 346-445), (partial) antibody (ABIN513800) Western Blot detection against Immunogen (36.74 KDa) .