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anti-Rabbit Caspase 9 antibody for Western Blotting

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Reactivity
Cow (Bovine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
397
109
106
15
13
9
5
4
2
2
2
1
1
1
Host
Rabbit
404
45
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Western Blotting (WB)
375
159
114
66
64
57
45
31
13
9
6
6
2
2
Supplier
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Catalog No. ABIN222874
$ 304.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011843324 ABIN126959 WB Rabbit Log in to see Polyclonal
0.0011843324 ABIN1002025 WB Rabbit Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Western Blotting

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Hamster Pig (Porcine) Rabbit
Western Blotting (WB) 329 Antibodies 93 Antibodies 91 Antibodies 5 Antibodies 6 Antibodies 3 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 57 Antibodies 10 Antibodies 1 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies 14 Antibodies 12 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 103 Antibodies 42 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Cow (Bovine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(397), (109), (106), (15), (13), (9), (5), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(404), (45), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Western Blotting (WB)
(375), (159), (114), (66), (64), (57), (45), (31), (13), (9), (6), (6), (2), (2)
Pubmed 3 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN222874) Images
Blocking Peptide Blocking peptide for this product available: ABIN412659
Specificity The antibody detects proform (~46 kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25 kDa) caspase-9.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 290 of human caspase-9
Isotype IgG

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN222874) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.
Synonyms: CASP-9 , EC 3.4.22.62 , ICE-like apoptotic protease 6 ,ICE-LAP6 ,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
Gene ID 842
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN222874) Images back to top
Application Notes Western blotting (0.5-4 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN222874) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-Caspase 9 Antibody (ABIN222874)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Product cited in:

Woods, Burns, Bradford, Ross, Kosyk, Swenberg, Cunningham, Rusyn: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007

Takebe, Cheng, Fandy, Srivastava, Wu, Shankar, Bauer, Shaughnessy, Tricot: "IMP dehydrogenase inhibitor mycophenolate mofetil induces caspase-dependent apoptosis and cell cycle inhibition in multiple myeloma cells." in: Molecular cancer therapeutics, Vol. 5, Issue 2, pp. 457-66, 2006

Siu, Alway: "Mitochondria-associated apoptotic signalling in denervated rat skeletal muscle." in: The Journal of physiology, Vol. 565, Issue Pt 1, pp. 309-23, 2005

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN222874) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN222874) Western blot analysis of Caspase 9with rat kidney tissue lysate.