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anti-Human EIF4EBP1 antibody for Immunofluorescence

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
C-Term
58
30
27
23
21
19
17
15
14
14
14
14
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
443
237
196
11
8
5
2
2
1
1
1
Host
Rabbit
388
95
4
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
16
11
10
10
8
8
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
320
202
199
89
61
27
18
15
14
6
5
4
3
3
3
1
Supplier
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Catalog No. ABIN499179
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2735693 ABIN1532584 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.2735693 ABIN2707290 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
0.2735693 ABIN1995479 IF ELISA Mouse IgG1 AA 2-118 Log in to see 5F3G9
0.2735693 ABIN2164534 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.2735693 ABIN560717 IF PLA ELISA WB Mouse IgG1 kappa AA 1-118, full length Log in to see 4F3-H2
0.2735693 ABIN4276857 ELISA ICC IF WB Rabbit C-Term Log in to see Polyclonal
0.2735693 ABIN4276858 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.2735693 ABIN2649165 IF Rabbit Log in to see
0.0011096579 ABIN197452 IF IHC (p) WB Rabbit AA 43-47 Log in to see Polyclonal 2
0.0011096579 ABIN152297 ICC IF IHC (p) IHC WB Rabbit C-Term Log in to see Polyclonal 45
0.0011096579 ABIN362872 IF IHC WB Rabbit IgG AA 43-47 Log in to see Polyclonal 5
0.0011096579 ABIN3183094 ELISA IF IHC Rabbit IgG Ser33 Log in to see Polyclonal
0.0011096579 ABIN303259 IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0011096579 ABIN1842405 IF IHC WB Rabbit IgG pThr45 Log in to see Polyclonal
0.0011096579 ABIN1978746 IF IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
0.0011096579 ABIN1978745 IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011096579 ABIN1827816 IF ELISA WB Mouse IgG1, kappa Log in to see 4F3-H2
0.0011096579 ABIN2885578 IF IHC ELISA Rabbit IgG AA 2-51 Log in to see Polyclonal
0.0011096579 ABIN4902797 IF IHC IP WB Mouse IgG Log in to see
0.0011096579 ABIN2132706 IF IHC ELISA WB Rabbit IgG pSer801 Log in to see Polyclonal

Top referenced anti-EIF4EBP1 antibody for Immunofluorescence

Similar anti-EIF4EBP1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 57 Antibodies 179 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 61 Antibodies
Immunohistochemistry (IHC) 102 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 141 Antibodies
Dot Blot (DB) 18 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope C-Term
(58), (30), (27), (23), (21), (19), (17), (15), (14), (14), (14), (14), (12), (12), (11), (10), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(443), (237), (196), (11), (8), (5), (2), (2), (1), (1), (1)
Host Rabbit
(388), (95), (4)
Clonality
Polyclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(19), (16), (11), (10), (10), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(320), (202), (199), (89), (61), (27), (18), (15), (14), (6), (5), (4), (3), (3), (3), (1)
Pubmed 2 references available
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN499179) Images
Specificity 4E-BP1 antibody was raised against a 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1.
Purification Affinity chromatography purified via peptide column
Immunogen A 14 amino acid peptide from near the carboxy-terminus of Human 4E-BP1
Isotype IgG

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN499179) Images back to top
Antigen
Alternative Name EIF4EBP1 / 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms (reviewed in 1). One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 2). One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs (1). Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 3), at least in part by inhibiting the activity of TOR towards 4E-BP1.Synonyms: Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-1, PHAS-I, PHAS1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1
Gene ID 1978
NCBI Accession NP_004086
UniProt Q13541
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN499179) Images back to top
Application Notes ELISA. Western Blot: 4E-BP1 antibody can be used for the detection of 4E-BP1 at 2.5 - 5 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN499179) Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-EIF4EBP1 Antibody (ABIN499179)

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Background publications

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003

Shah, Anthony, Kimball, Jefferson: "4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle." in: American journal of physiology. Endocrinology and metabolism, Vol. 279, Issue 4, pp. E715-29, 2000

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN499179) back to top
Supplier Images
Immunofluorescence (IF) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (C-Term) (ABIN499179) Immunocytochemistry of AP30001PU-N 4E-BP1 antibody in 3T3 cells with 4E-BP1 antibody ...
Western Blotting (WB) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (C-Term) (ABIN499179) Western blot analysis of AP30001PU-N 4E-BP1 antibody in 3T3 cell lysate with 4E-BP1 a...