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anti-Human EIF4EBP1 antibody for Western Blotting

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
AA 48-77, pThr69
59
30
29
22
20
20
17
14
13
13
13
13
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
435
238
190
10
8
5
2
2
1
1
1
Host
Rabbit
383
91
9
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
313
200
192
86
56
26
21
18
14
7
5
4
4
3
3
1
Supplier
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Catalog No. ABIN388707
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN659184 WB Mouse IgG1 Log in to see 319CT22-8-2 2
0.13476625 ABIN189001 FACS IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y330 1
0.13476625 ABIN950187 EIA WB Rabbit Ig Middle Region Log in to see Polyclonal 2
0.13476625 ABIN499179 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 2
0.13476625 ABIN968937 ELISA WB Mouse IgG1 Log in to see 9E12D9 2
0.13476625 ABIN969467 IHC ELISA WB Mouse IgG1 Log in to see 11G12C11 2
0.13476625 ABIN1107077 IHC (p) WB Mouse IgG1 Log in to see 4B6G10 2
0.13476625 ABIN1107078 IHC (p) WB Mouse IgG1 Log in to see 11G12C11 2
0.13476625 ABIN1107076 WB Mouse IgG1 Log in to see 9E12D9 2
0.13476625 ABIN1724657 IHC ELISA WB Mouse IgG1 Log in to see 4B6G10 2
0.13476625 ABIN1531763 IHC ELISA WB Rabbit IgG pThr36 Log in to see Polyclonal
0.13476625 ABIN1531764 IHC ELISA WB Rabbit IgG pThr45 Log in to see Polyclonal
0.13476625 ABIN1531250 IHC ELISA WB Rabbit IgG pSer64 Log in to see Polyclonal
0.13476625 ABIN498732 IHC (p) WB Rabbit pThr37 Log in to see Polyclonal
0.13476625 ABIN498733 IHC (p) WB Rabbit pThr46 Log in to see Polyclonal
0.13476625 ABIN1532268 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN1532792 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN1532793 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2784742 WB Rabbit N-Term Log in to see Polyclonal
0.13476625 ABIN498611 IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-EIF4EBP1 antibody for Western Blotting

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Application / Reactivity Human
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ELISA 173 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 193 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 86 Antibodies
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Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 300 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope AA 48-77, pThr69
(59), (30), (29), (22), (20), (20), (17), (14), (13), (13), (13), (13), (12), (12), (11), (10), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(435), (238), (190), (10), (8), (5), (2), (2), (1), (1), (1)
Host Rabbit
(383), (91), (9)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EIF4EBP1 antibody is un-conjugated
(20), (17), (12), (11), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(313), (200), (192), (86), (56), (26), (21), (18), (14), (7), (5), (4), (4), (3), (3), (1)
Pubmed 2 references available
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN388707) Images
Specificity This EIF4EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T69 of human EIF4EBP1.
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This EIF4EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 AA from human EIF4EBP1.
Clone RB11407
Isotype Ig

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN388707) Images back to top
Antigen
Alternative Name EIF4EBP1 (EIF4EBP1 Antibody Abstract)
Background EIF4EBP1 is one member of a family of translation repressor proteins. It directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Synonyms: 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I
Molecular Weight 12449 DA
Gene ID 1978
NCBI Accession NP_004086
Research Area Translation Factors, Signaling
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN388707) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN388707) Images back to top
Format Liquid
Concentration 0.29 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-EIF4EBP1 Antibody (ABIN388707)

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Background publications

Fonseca, Smith, Lee, MacKintosh, Proud: "PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex." in: The Journal of biological chemistry, Vol. 282, Issue 34, pp. 24514-24, 2007

Armengol, Rojo, Castellví, Iglesias, Cuatrecasas, Pons, Baselga, Ramón y Cajal: "4E-binding protein 1: a key molecular "funnel factor" in human cancer with clinical implications." in: Cancer research, Vol. 67, Issue 16, pp. 7551-5, 2007

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN388707) back to top
Supplier Images
Western Blotting (WB) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (AA 48-77) (ABIN388707) Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (...
Immunohistochemistry (IHC) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (AA 48-77) (ABIN388707) Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with EIF4EB...