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anti-Mouse (Murine) EIF4EBP1 antibody for Flow Cytometry

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
pThr36, pThr45
58
30
28
22
20
20
17
14
14
13
13
13
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
435
237
190
10
8
5
2
2
1
1
1
Host
Mouse
384
90
9
Clonality
Monoclonal
Conjugate
This EIF4EBP1 antibody is conjugated to EFluor 660
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
314
200
192
87
56
26
20
18
14
6
5
4
4
3
3
1
Supplier
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Available images

Catalog No. ABIN2677045
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30219433 ABIN2677147 FACS PerCP-eFluor® 710 Rat IgG2a kappa Log in to see 6C3
0.0010481157 ABIN354772 FACS IF IHC (p) WB Rabbit IgG pThr37, pThr46 Log in to see
0.0010481157 ABIN280960 FACS IP IHC Rat IgG2a kappa Log in to see 6C3
0.0010481157 ABIN279837 FACS Biotin Rat IgG2a kappa Log in to see 6C3
0.0010481157 ABIN278063 FACS FITC Rat IgG2a Log in to see 6C3
0.0010481157 ABIN278961 FACS PE Rat IgG2a kappa Log in to see 6C3
0.0010481157 ABIN2211697 FACS FITC Rat IgG1 kappa Log in to see 5K22
0.0010481157 ABIN2211698 FACS Biotin Rat IgG1 kappa Log in to see 5K22
0.0010481157 ABIN2211699 FACS PE Rat IgG1 kappa Log in to see 5K22
0.0010481157 ABIN2211696 FACS Rat IgG1 kappa Log in to see 5K22
0.0010481157 ABIN3014095 FACS PE Mouse IgG2b kappa pThr36, pThr45 Log in to see V3NTY24

Similar anti-EIF4EBP1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 57 Antibodies 173 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 104 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Western Blotting (WB) 141 Antibodies
Dot Blot (DB) 18 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope pThr36, pThr45
(58), (30), (28), (22), (20), (20), (17), (14), (14), (13), (13), (13), (12), (12), (11), (10), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(435), (237), (190), (10), (8), (5), (2), (2), (1), (1), (1)
Host Mouse
(384), (90), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EIF4EBP1 antibody is conjugated to EFluor 660
(20), (17), (12), (11), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(314), (200), (192), (87), (56), (26), (20), (18), (14), (6), (5), (4), (4), (3), (3), (1)
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling Images
Specificity The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46. 4E-BP1 is a member of a family of translation repressor proteins that include 4E-BP2 and 4E-BP3. In its non-phosphorylated form, 4E-BP1 binds to the eIF4E translation initiation factor and represses cap-dependent translation. Phosphorylation of 4E-BP1 at multiple sites is necessary to disrupt this interaction and de-repress cap-dependent translation. Studies have identified several kinases that phosphorylate 4E-BP1. For instance, FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111. Phosphorylation of 4E-BP1 at Thr37 and Thr46 is inhibited by the PI3 kinase inhibitors LY294002 and wortmannin.
Characteristics The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E- binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46. 4E-BP1 is a member of a family of translation repressor proteins that include 4E-BP2 and 4E-BP3. In its non-phosphorylated form, 4E-BP1 binds to the eIF4E translation initiation factor and represses cap-dependent translation. Phosphorylation of 4E-BP1 at multiple sites is necessary to disrupt this interaction and de-repress cap-dependent translation. Studies have identified several kinases that phosphorylate 4E-BP1. For instance, FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111. Phosphorylation of 4E-BP1 at Thr37 and Thr46 is inhibited by the PI3 kinase inhibitors LY294002 and wortmannin.
Clone V3NTY24
Isotype IgG2b kappa

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling Images back to top
Application Notes Applications reported: This V3NTY24 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This V3NTY24 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Protocols: Use of Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins is recommended and allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol may be used and allows for the greatest discrimination of phospho- specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). Use of Protocol B: One-step protocol for intracellular (nuclear) proteins is not recommended. All Protocols can be found in the "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red Page 2 of 2 Anti-Human/Mouse phospho-4E-BP1 (T36/T45) eFluor® 660
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr36) (EFluor 660) (ABIN2677045) anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pThr36), (pThr45) antibody (EFluor 660)