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anti-Mouse (Murine) EIF4EBP1 antibody for Western Blotting

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
pThr69
59
30
29
22
21
20
17
14
13
13
13
13
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
439
239
191
11
8
5
2
2
1
1
1
Host
Rabbit
385
92
9
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
316
201
192
89
56
27
21
18
15
7
5
4
4
3
3
1
Supplier
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Catalog No. ABIN1531251
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN499179 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 2
0.13341247 ABIN1531763 IHC ELISA WB Rabbit IgG pThr36 Log in to see Polyclonal
0.13341247 ABIN1531764 IHC ELISA WB Rabbit IgG pThr45 Log in to see Polyclonal
0.13341247 ABIN1531250 IHC ELISA WB Rabbit IgG pSer64 Log in to see Polyclonal
0.13341247 ABIN498732 IHC (p) WB Rabbit pThr37 Log in to see Polyclonal
0.13341247 ABIN498733 IHC (p) WB Rabbit pThr46 Log in to see Polyclonal
0.13341247 ABIN1532268 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1532792 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1532793 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2784742 WB Rabbit N-Term Log in to see Polyclonal
0.13341247 ABIN498611 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN498612 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN2162272 IHC WB Rabbit IgG Thr239, Thr45 Log in to see Polyclonal
0.13341247 ABIN2162271 IHC WB Rabbit IgG Thr36, Tyr170 Log in to see Polyclonal
0.13341247 ABIN1532269 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2705357 IHC WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN1870153 IHC WB Rabbit IgG pThr36 Log in to see Polyclonal
0.13341247 ABIN2705356 IHC WB Rabbit pSer69 Log in to see Polyclonal
0.13341247 ABIN2707288 IHC WB Rabbit N-Term, pSer37 Log in to see Polyclonal
0.13341247 ABIN2707289 IHC WB Rabbit N-Term, pSer46 Log in to see Polyclonal

Top referenced anti-EIF4EBP1 antibody for Western Blotting

Similar anti-EIF4EBP1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 46 Antibodies 57 Antibodies
Gel Shift (GS) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 102 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 121 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 12 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope pThr69
(59), (30), (29), (22), (21), (20), (17), (14), (13), (13), (13), (13), (12), (12), (11), (10), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(439), (239), (191), (11), (8), (5), (2), (2), (1), (1), (1)
Host Rabbit
(385), (92), (9)
Clonality
Polyclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(20), (17), (12), (11), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(316), (201), (192), (89), (56), (27), (21), (18), (15), (7), (5), (4), (4), (3), (3), (1)
Pubmed 1 reference available
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) Images
Specificity 4E-BP1 (Phospho-Thr69) Antibody detects endogenous levels of 4E- BP1 only when phosphorylated at Thr69.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69.
Isotype IgG

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) Images back to top
Antigen
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background Molecular weight of target protein: 12 kDa
Synonyms: 4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I
Molecular Weight 12 kDa
Gene ID 13685
UniProt Q60876
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Mm.6700 (NCBI Gene Symbol: EIF4EBP1)

Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN1531251) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-EIF4EBP1 Antibody (ABIN1531251)

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Background publications

Pause, Belsham, Gingras, Donzé, Lin, Lawrence, Sonenberg: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, 1994

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) back to top
Supplier Images
Western Blotting (WB) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr69) (ABIN1531251) Western blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 30', us...
Immunohistochemistry (IHC) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr69) (ABIN1531251) Immunohistochemistry analysis of paraffin-embedded human brain carcinoma, using 4E-BP...