You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Chicken GJA1 antibody for Immunoprecipitation

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
AA 252-270
66
21
20
16
15
12
10
8
7
7
6
6
6
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
257
195
175
55
24
22
22
20
18
17
10
10
7
7
7
5
4
4
4
3
3
3
3
2
2
1
1
1
Host
Mouse
255
26
23
Clonality
Monoclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
238
121
104
73
42
19
14
13
11
7
7
5
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN967717
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967718 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
1 ABIN2704673 IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2621332 IP WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-GJA1 antibody for Immunoprecipitation

Similar anti-GJA1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 151 Antibodies 170 Antibodies 193 Antibodies 39 Antibodies 22 Antibodies
Immunoprecipitation (IP) 4 Antibodies 4 Antibodies 9 Antibodies 9 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope AA 252-270
(66), (21), (20), (16), (15), (12), (10), (8), (7), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(257), (195), (175), (55), (24), (22), (22), (20), (18), (17), (10), (10), (7), (7), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(255), (26), (23)
Clonality (Clone)
Monoclonal   ( )
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(238), (121), (104), (73), (42), (19), (14), (13), (11), (7), (7), (5), (2), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN967717) Images
Cross-Reactivity Chicken, Dog (Canine), Human, Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat Connexin-43 aa. 252-270
Clone 2-Connexin
Isotype IgG1

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN967717) Images back to top
Antigen
Alternative Name Connexin-43 (GJA1 Antibody Abstract)
Background Gap junctions are intercellular protein pores. They enable cell-to-cell communication by allowing passage of ions and other small molecules. The subunits of gap junction channels are assembled from a family of proteins called connexins. Individual connexin molecules join to make hexameric hemichannels termed connexons, these structures dock to connexons on neighboring cells, forming gap junction pores. Connexin-43 is a member of the connexin family possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. In addition, it is believed that phosphorylation of connexin-43 plays a regulatory role both in the assembly of connexons and in gating activity at the gap junction. This antibody is routinely tested by western blot analysis.
Molecular Weight 43 kDa
Research Area Extracellular Matrix, Cytoskeleton, Signaling
Pathways MAPK Signaling, Myometrial Relaxation and Contraction

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN967717) Images back to top
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN967717) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-GJA1 Antibody (ABIN967717)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Product cited in:

Giepmans, Hengeveld, Postma, Moolenaar: "Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8544-9, 2001

Toyofuku, Akamatsu, Zhang, Kuzuya, Tada, Hori: "c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes." in: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 1780-8, 2001

Lucke, Choudhry, Thom, Selmer, Burden, Hodgins: "Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium." in: The Journal of investigative dermatology, Vol. 112, Issue 3, pp. 354-61, 1999

Laird, Castillo, Kasprzak: "Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1193-203, 1996

Loo, Berestecky, Kanemitsu, Lau: "pp60src-mediated phosphorylation of connexin 43, a gap junction protein." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12751-61, 1995

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN967717) back to top
Supplier Images
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270) (ABIN967717) Western blot analysis of Connexin-43 on a rat cerebrum lysate. Lane 1: 1:250, lane 2:...
Immunofluorescence (IF) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270) (ABIN967717) Immunofluorescence staining on WI38 cells (human lung).