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anti-Mouse (Murine) GJA1 antibody for Enzyme Immunoassay

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • AI118175
  • AU042049
  • AVSD3
  • AW546267
  • Cnx32
  • Cnx43
  • Cnx46
  • connexin43
  • connexin 43
  • Cx32
  • cx43
  • CX43
  • Cx43
  • cx43a1
  • Cx43alpha1
  • Cx46
  • DFNB38
  • dfnb38
  • Gja-1
  • Gja-3
  • gja1
  • gja1-A
  • GJAL
  • gjal
  • Gjb-1
  • HLHS1
  • HSS
  • Npm1
  • ODDD
  • oddd
  • zfCx43.3
Reactivity
Human, Mouse (Murine), Rat (Rattus)
242
163
140
44
20
15
14
12
11
10
9
7
6
6
5
4
4
3
3
3
3
3
2
1
1
1
Host
Rabbit
245
28
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
220
114
103
58
43
20
12
10
10
9
7
2
2
1
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Available images

Catalog No. ABIN265368
$ 390.50
Plus shipping costs $45.00

Similar anti-GJA1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 45 Antibodies 45 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 3 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Light Microscopy (LM) 2 Antibodies
Western Blotting (WB) 143 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(242), (163), (140), (44), (20), (15), (14), (12), (11), (10), (9), (7), (6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(245), (28), (22)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(220), (114), (103), (58), (43), (20), (12), (10), (10), (9), (7), (2), (2), (1)
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling Images
Specificity This antibody detects endogenous levels of GJA1 / Cx43 protein(region surrounding Ser369).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen.

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GJA1 / Cx43 (GJA1 Antibody Abstract)
Background Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
Molecular Weight approx. 43 kDa
Gene ID 2697
NCBI Accession NP_000156
UniProt P17302
Pathways MAPK Signaling

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling Images back to top
Application Notes ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid Repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling back to top
Supplier Images
 image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (ABIN265368) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) antibody
 image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (ABIN265368) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) antibody (Image 2)