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|Application / Reactivity||Human||Mouse (Murine)||Dog (Canine)|
|Western Blotting (WB)||199 Antibodies||88 Antibodies||5 Antibodies|
|Immunoprecipitation (IP)||18 Antibodies||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||41 Antibodies||26 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||152 Antibodies||69 Antibodies||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunofluorescence (IF)||62 Antibodies||34 Antibodies||2 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine) Alternatives|
|Conjugate||This MAP2K4 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-MAP2K4 AntibodyTarget Details MAP2K4 Application Details Handling Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412828|
|Specificity||The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human, mouse, and dog origins.|
|Characteristics||Affinity purified polyclonal antibody to detect SEK1 (MKK4/JKK1) in human, mouse and dog samples.|
|Immunogen||Synthetic peptide surrounding amino acid 347 of mouse SEK1|
Target Details MAP2K4Product Details anti-MAP2K4 Antibody Application Details Handling Images back to top
|Alternative Name||SEK1/MKK4/JKK1 (MAP2K4 Antibody Abstract)|
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs throμgh phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
Synonyms: NKK , JNKK1 , MAP kinase kinase 4 , MAP2K4 , MAPK/ERK kinase 4 , MAPKK4 , MEK4 , monkeyK4 , PRKMK4 , SAPK/ERK kinase 1 , SEK1 ,SERK1
|Pathways||MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Handling Images back to top
Western blotting (0.5-4 μg/mL).
However, the optimal concentrations should be determined individually.
Suggested positive control: Western Blot: Jurkat cell lysate
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
ImagesProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Handling back to top