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anti-Dog (Canine) MAP2K4 antibody for Western Blotting

Recommended MAP2K4 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies
  • 2
  • 142758_at
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4
  • ATMEK4
  • ATMKK4
  • CG9738
  • CT27508
  • CT38386
  • D-MKK4
  • DmelCG12798
  • Dmel\\CG9738
  • dMkk4
  • DMKK4
  • dMKK4
  • F19C24.26
  • F19C24_26
  • hep
  • jnkk
  • JNKK
  • JNKK1
  • jnkk1
  • Jnkk2
  • JNKK2
  • map2k4
  • MAP KINASE KINASE 4
  • MAPKK4
  • mapkk4
  • Mapkk7
  • MEK4
  • mek4
  • Mek7
  • mitogen-activated protein kinase kinase 4
  • MKK4
  • mkk4
  • MKK4A-l
  • Mkk7
  • Mpk4
  • PRKMK4
  • prkmk4
  • SAPKK-1
  • SAPKK1
  • Sek1
  • sek1
  • SEK1
  • SEK1/MKK4
  • serk1
  • SERK1
  • Serk1
  • SKK1
  • xmek2
  • zgc:136500
Reactivity
Dog (Canine), Human, Mouse (Murine)
304
145
115
9
8
4
4
2
Host
Rabbit
280
37
Clonality
Polyclonal
Conjugate
This MAP2K4 antibody is un-conjugated
12
12
11
8
8
8
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
205
149
117
62
41
18
14
13
10
10
4
3
1
Supplier
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Available images

Catalog No. ABIN223508
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN783859 WB Rabbit Log in to see Polyclonal
0.0011760078 ABIN2452426 FACS IF IP IHC WB Mouse IgG2b Log in to see 8A8
0.0011760078 ABIN1003131 WB Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN4334806 FACS ICC IF IHC IHC (p) IP WB Mouse IgG2b Log in to see 8A8

Similar anti-MAP2K4 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine)
Western Blotting (WB) 199 Antibodies 90 Antibodies 5 Antibodies
Immunoprecipitation (IP) 18 Antibodies 1 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies 27 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 150 Antibodies 70 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunofluorescence (IF) 62 Antibodies 34 Antibodies 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine)
(304), (145), (115), (9), (8), (4), (4), (2)
Host Rabbit
(280), (37)
Clonality
Polyclonal
Conjugate This MAP2K4 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(205), (149), (117), (62), (41), (18), (14), (13), (10), (10), (4), (3), (1)
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Product Details anti-MAP2K4 Antibody

Target Details MAP2K4 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN412828
Specificity The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human, mouse, and dog origins.
Characteristics Affinity purified polyclonal antibody to detect SEK1 (MKK4/JKK1) in human, mouse and dog samples.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 347 of mouse SEK1
Isotype IgG

Target Details MAP2K4

Product Details anti-MAP2K4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SEK1/MKK4/JKK1 (MAP2K4 Antibody Abstract)
Background SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs throμgh phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
Synonyms: NKK , JNKK1 , MAP kinase kinase 4 , MAP2K4 , MAPK/ERK kinase 4 , MAPKK4 , MEK4 , monkeyK4 , PRKMK4 , SAPK/ERK kinase 1 , SEK1 ,SERK1
Gene ID 26398
Pathways MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Handling Images back to top
Application Notes Western blotting (0.5-4 μg/mL).
However, the optimal concentrations should be determined individually.
Comment

Suggested positive control: Western Blot: Jurkat cell lysate
Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

Images

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K4 antibody (Mitogen-Activated Protein Kinase Kinase 4) (ABIN223508) Western blot analysis of SEK1 expression with Jurkat cell lysate (Lane 1, 2).