|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||28 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||42 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) Antibodies|
|Epitope||AA 1065-1097, Middle Region Alternatives|
|Conjugate||This MAP3K4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAP3K4 AntibodyTarget Details MAP3K4 Application Details Handling References for anti-MAP3K4 Antibody (ABIN953298) Images
|Specificity||This antibody reacts to MEKK4.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Protein A column, followed by peptide affinity purification|
|Immunogen||KLH conjugated synthetic peptide between 1065-1097 amino acids from the Central region of human MEKK4 Genename: MAP3K4|
Target Details MAP3K4Product Details anti-MAP3K4 Antibody Application Details Handling References for anti-MAP3K4 Antibody (ABIN953298) Images back to top
|Alternative Name||MAP3K4 (MAP3K4 Antibody Abstract)|
|Background||The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.Synonyms: MAP three kinase 1, MAPK/ERK kinase kinase 4, MAPKKK4, MEKK4, MTK1, Mitogen-activated protein kinase kinase kinase 4|
|Molecular Weight||181685 Da|
Application DetailsProduct Details anti-MAP3K4 Antibody Target Details MAP3K4 Handling References for anti-MAP3K4 Antibody (ABIN953298) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K4 Antibody Target Details MAP3K4 Application Details References for anti-MAP3K4 Antibody (ABIN953298) Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-MAP3K4 Antibody (ABIN953298)Product Details anti-MAP3K4 Antibody Target Details MAP3K4 Application Details Handling Images back to top
Grucza, Johnson, Krueger et al.: "Incorporating age at onset of smoking into genetic models for nicotine dependence: evidence for interaction with multiple genes." in: Addiction biology, Vol. 15, Issue 3, pp. 346-57, 2010 (PubMed).
Whitmarsh, Davis: "Role of mitogen-activated protein kinase kinase 4 in cancer." in: Oncogene, Vol. 26, Issue 22, pp. 3172-84, 2007 (PubMed).
Aissouni, Zapart, Iovanna et al.: "CIN85 regulates the ability of MEKK4 to activate the p38 MAP kinase pathway." in: Biochemical and biophysical research communications, Vol. 338, Issue 2, pp. 808-14, 2005 (PubMed).
Abell, Johnson: "MEKK4 is an effector of the embryonic TRAF4 for JNK activation." in: The Journal of biological chemistry, Vol. 280, Issue 43, pp. 35793-6, 2005 (PubMed).
Derbyshire, Halfter, Heimark et al.: "Angiotensin II stimulated transcription of cyclooxygenase II is regulated by a novel kinase cascade involving Pyk2, MEKK4 and annexin II." in: Molecular and cellular biochemistry, Vol. 271, Issue 1-2, pp. 77-90, 2005 (PubMed).
ImagesProduct Details anti-MAP3K4 Antibody Target Details MAP3K4 Application Details Handling References for anti-MAP3K4 Antibody (ABIN953298) back to top