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anti-Human MAP3K4 antibody for Western Blotting

Recommended MAP3K4 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) Antibodies
  • D17Rp17
  • D17Rp17e
  • MAPKKK4
  • Mek4b
  • Mekk4
  • MEKK 4
  • MEKK4
  • mKIAA0213
  • MTK1
  • PRO0412
  • RP17
  • Rp17a
  • Tas
Epitope
AA 1065-1097, Middle Region
18
15
13
10
8
8
7
2
2
1
1
1
1
Reactivity
Human
78
30
23
Host
Rabbit
57
21
Clonality
Polyclonal
Conjugate
This MAP3K4 antibody is un-conjugated
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
48
46
35
12
11
4
2
2
1
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Catalog No. ABIN953298
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13309549 ABIN393795 WB Mouse IgG1 kappa AA 1201-1300 Log in to see 4F10 5
0.13309549 ABIN393852 WB Mouse IgG1 kappa AA 1201-1300 Log in to see 6C6 5
0.13309549 ABIN2704980 IHC WB Rabbit C-Term Log in to see Polyclonal
0.13309549 ABIN658707 IHC (p) WB Rabbit Ig AA 1067-1097, Center, Cys1081 Log in to see Polyclonal
0.13309549 ABIN1327304 ELISA WB Mouse IgG2b AA 1201-1300, partial Log in to see X1
0.13309549 ABIN950015 ELISA WB Mouse IgG2b AA 1201-1300, partial Log in to see X1
0.13309549 ABIN561799 ELISA WB Mouse IgG1 kappa AA 1201-1300, partial Log in to see 4C3
0.13309549 ABIN561798 IHC (p) ELISA WB Mouse IgG1 kappa AA 1201-1300, partial Log in to see 6C6
0.13309549 ABIN561801 IHC (p) ELISA WB Mouse IgG1 kappa AA 1201-1300, partial Log in to see 4F10
0.13309549 ABIN2445622 IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.0011733918 ABIN598811 IHC (p) ELISA WB Mouse IgG1, kappa Log in to see 6C6
0.0011733918 ABIN598812 IHC (p) ELISA WB Mouse IgG1, kappa Log in to see 4F10
0.0011733918 ABIN1911734 IHC ELISA WB Biotin Rabbit IgG Internal Region, AA 1065-1097 Log in to see Polyclonal
0.0011733918 ABIN1911735 IHC ELISA WB FITC Rabbit IgG Internal Region, AA 1065-1097 Log in to see Polyclonal
0.0011733918 ABIN1911733 IHC ELISA WB APC Rabbit IgG Internal Region, AA 1065-1097 Log in to see Polyclonal
0.0011733918 ABIN1911736 IHC ELISA WB PE Rabbit IgG Internal Region, AA 1065-1097 Log in to see Polyclonal
0.0011733918 ABIN1911732 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Internal Region, AA 1065-1097 Log in to see Polyclonal
0.0011733918 ABIN1911737 IHC ELISA WB HRP Rabbit IgG Internal Region, AA 1065-1097 Log in to see Polyclonal
0.0011733918 ABIN561802 ELISA WB Mouse IgG2b kappa AA 1201-1300, partial Log in to see 5D4
0.0011733918 ABIN561800 PLA ELISA WB Mouse IgG1 kappa AA 1201-1300, partial Log in to see 6A12

Top referenced anti-MAP3K4 antibody for Western Blotting

Similar anti-MAP3K4 Antibodies

Application / Reactivity Human
ELISA 46 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 49 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) Antibodies
Epitope AA 1065-1097, Middle Region
(18), (15), (13), (10), (8), (8), (7), (2), (2), (1), (1), (1), (1)
Reactivity Human
(78), (30), (23)
Host Rabbit
(57), (21)
Clonality
Polyclonal
Conjugate This MAP3K4 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(48), (46), (35), (12), (11), (4), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-MAP3K4 Antibody

Target Details MAP3K4 Application Details Handling References for anti-MAP3K4 Antibody (ABIN953298) Images
Specificity This antibody reacts to MEKK4.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein A column, followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 1065-1097 amino acids from the Central region of human MEKK4 Genename: MAP3K4
Isotype Ig

Target Details MAP3K4

Product Details anti-MAP3K4 Antibody Application Details Handling References for anti-MAP3K4 Antibody (ABIN953298) Images back to top
Antigen
Alternative Name MAP3K4 (MAP3K4 Antibody Abstract)
Background The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.Synonyms: MAP three kinase 1, MAPK/ERK kinase kinase 4, MAPKKK4, MEKK4, MTK1, Mitogen-activated protein kinase kinase kinase 4
Molecular Weight 181685 Da
Gene ID 4216
NCBI Accession NP_005913
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K4 Antibody Target Details MAP3K4 Handling References for anti-MAP3K4 Antibody (ABIN953298) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K4 Antibody Target Details MAP3K4 Application Details References for anti-MAP3K4 Antibody (ABIN953298) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MAP3K4 Antibody (ABIN953298)

Product Details anti-MAP3K4 Antibody Target Details MAP3K4 Application Details Handling Images back to top
Background publications

Grucza, Johnson, Krueger, Breslau, Saccone, Chen, Derringer, Agrawal, Lynskey, Bierut: "Incorporating age at onset of smoking into genetic models for nicotine dependence: evidence for interaction with multiple genes." in: Addiction biology, Vol. 15, Issue 3, pp. 346-57, 2010

Whitmarsh, Davis: "Role of mitogen-activated protein kinase kinase 4 in cancer." in: Oncogene, Vol. 26, Issue 22, pp. 3172-84, 2007

Aissouni, Zapart, Iovanna, Dikic, Soubeyran: "CIN85 regulates the ability of MEKK4 to activate the p38 MAP kinase pathway." in: Biochemical and biophysical research communications, Vol. 338, Issue 2, pp. 808-14, 2005

Abell, Johnson: "MEKK4 is an effector of the embryonic TRAF4 for JNK activation." in: The Journal of biological chemistry, Vol. 280, Issue 43, pp. 35793-6, 2005

Derbyshire, Halfter, Heimark, Sy, Vaillancourt: "Angiotensin II stimulated transcription of cyclooxygenase II is regulated by a novel kinase cascade involving Pyk2, MEKK4 and annexin II." in: Molecular and cellular biochemistry, Vol. 271, Issue 1-2, pp. 77-90, 2005

Images

Product Details anti-MAP3K4 Antibody Target Details MAP3K4 Application Details Handling References for anti-MAP3K4 Antibody (ABIN953298) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MAP3K4 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 4) (AA 1065-1097) (ABIN953298) Formalin fixed and paraffin embedded human heart stained with MEKK4 Antibody (Center)...
Western Blotting (WB) image for anti-MAP3K4 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 4) (AA 1065-1097) (ABIN953298) Western blot analysis of MEKK4 Antibody (Center) Cat.-No AP52665PU-N in human normal ...