anti-Human MAP3K5 antibody for Immunohistochemistry

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Epitope
AA 932-981, pSer966
72
58
37
19
19
15
15
15
14
13
10
7
6
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
400
171
96
6
1
1
1
1
1
1
Host
Rabbit
379
27
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
304
159
133
82
65
43
21
13
12
10
4
3
2
1
1
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Catalog No. ABIN1531773
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.869368 ABIN1532802 IHC ELISA WB Rabbit IgG AA 932-981 Log in to see Polyclonal 2
8.869368 ABIN2705520 IHC WB Rabbit Center Log in to see Polyclonal
8.869368 ABIN2707341 IHC WB Rabbit N-Term Log in to see Polyclonal
8.869368 ABIN1531772 IHC ELISA WB Rabbit IgG AA 49-98, pSer83 Log in to see Polyclonal
8.869368 ABIN1532801 IHC ELISA WB Rabbit IgG AA 49-98 Log in to see Polyclonal
8.869368 ABIN969273 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 2-00E-04 1
8.869368 ABIN4281869 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
8.869368 ABIN1870370 IHC WB Rabbit IgG pSer966 Log in to see Polyclonal
8.869368 ABIN1870371 IHC WB Rabbit IgG pSer83 Log in to see Polyclonal
8.869368 ABIN4281875 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 2E4
8.869368 ABIN2431625 IHC ELISA Rabbit IgG Log in to see Polyclonal
8.869368 ABIN1844371 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
8.869368 ABIN3019770 IHC WB Rabbit pSer966 Log in to see Polyclonal
8.869368 ABIN3020331 IHC WB Rabbit pSer83 Log in to see Polyclonal
1 ABIN2755775 IHC WB Rabbit N-Term, pSer83 Log in to see Polyclonal
1 ABIN362730 IF IHC Rabbit IgG AA 964-968 Log in to see Polyclonal 5
1 ABIN362714 IF IHC WB Rabbit IgG AA 81-85 Log in to see Polyclonal 4
1 ABIN362196 IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 5
1 ABIN362194 IHC WB Rabbit IgG pSer83 Log in to see Polyclonal 5
1 ABIN3197893 IHC WB Rabbit pSer966 Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope AA 932-981, pSer966
(72), (58), (37), (19), (19), (15), (15), (15), (14), (13), (10), (7), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(400), (171), (96), (6), (1), (1), (1), (1), (1), (1)
Host Rabbit
(379), (27)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(304), (159), (133), (82), (65), (43), (21), (13), (12), (10), (4), (3), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN1531773) Images
Specificity

ASK1 (Phospho-Ser966) Antibody detects endogenous levels of ASK1 only when phosphorylated at Ser966.

PhosphorylationH:S966 M:S973 R:S973

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 antibody (ABIN1531773) Images back to top
Antigen
Alternative Name ASK1 (MAP3K5 Antibody Abstract)
Background

Synonyms: Apoptosis signal-regulating kinase 1, ASK-1, kinase ASK1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Mitogen-activated protein kinase kinase kinase 5

NCBI Gene Symbol: MAP3K5

Molecular Weight 154 kDa
Gene ID 4217
OMIM 602448
UniProt Q99683
Research Area Cardiovascular, Hypertrophy, Apoptosis/Necrosis
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 antibody (ABIN1531773) Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Comment

Unigene-Number: Hs.186486 (NCBI Gene Symbol: MAP3K5)

Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 antibody (ABIN1531773) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-MAP3K5 antibody (ABIN1531773)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Mungall, Palmer, Sims, Edwards, Ashurst, Wilming, Jones, Horton, Hunt, Scott, Gilbert, Clamp, Bethel, Milne, Ainscough, Almeida, Ambrose, Andrews, Ashwell, Babbage, Bagguley, Bailey, Banerjee, Barker et al.: "The DNA sequence and analysis of human chromosome 6. ..." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN1531773) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 932-981), (pSer966) antibody (ABIN1531773) Western blot analysis of extracts from 293 cells treated with TNF(20ng/ml)+calyculinA...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 932-981), (pSer966) antibody (ABIN1531773) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1...