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anti-Mouse (Murine) MAP3K5 antibody for Enzyme Immunoassay

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Epitope
N-Term
53
48
43
23
22
20
17
15
13
13
13
9
8
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
374
139
78
4
1
Host
Rabbit
359
23
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
287
157
136
59
55
40
23
10
8
7
6
2
1
1
1
Supplier
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Catalog No. ABIN499349
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN499347 EIA IF WB Rabbit IgG Log in to see Polyclonal 3
0.0008867729 ABIN265316 EIA IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-MAP3K5 antibody for Enzyme Immunoassay

Similar anti-MAP3K5 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 24 Antibodies 132 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 11 Antibodies
Flow Cytometry (FACS) 1 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 11 Antibodies 23 Antibodies
Immunofluorescence (IF) 14 Antibodies 41 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 55 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Western Blotting (WB) 81 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope N-Term
(53), (48), (43), (23), (22), (20), (17), (15), (13), (13), (13), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(374), (139), (78), (4), (1)
Host Rabbit
(359), (23)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(287), (157), (136), (59), (55), (40), (23), (10), (8), (7), (6), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499349) Images
Specificity ASK1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human ASK1.
Purification Affinity chromatography purified via peptide column
Immunogen Human ASK1 (N-Terminus) Peptide
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN499349) Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-?-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN499349) Images back to top
Application Notes ELISA. Western Blot: ASK1 antibody can be used for the detection of ASK1 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN499349) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-MAP3K5 Antibody (ABIN499349)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Wang, Diener, Jannuzzi, Trollinger, Tan, Lichenstein, Zukowski, Yao: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499349) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (N-Term) (ABIN499349) Western blot analysis of ASK1 in 3T3 cell lysate with AP30087PU-N ASK1 antibody at (A...