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anti-Mouse (Murine) MAPK3 antibody for Enzyme Immunoassay

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Epitope
N-Term
58
44
41
39
27
20
20
15
14
13
9
9
8
8
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
454
216
155
27
23
22
19
19
16
13
7
6
4
4
4
4
3
3
2
2
2
1
1
Host
Rabbit
317
151
21
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
23
23
21
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
384
184
151
96
66
57
50
33
30
18
9
6
4
3
2
2
2
1
1
Supplier
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Catalog No. ABIN359630
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008854307 ABIN126829 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4
0.0008854307 ABIN401356 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008854307 ABIN126831 EIA IHC (fro) IF IP WB Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4
0.0008854307 ABIN1108133 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1-00E-005

Top referenced anti-MAPK3 antibody for Enzyme Immunoassay

Similar anti-MAPK3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 26 Antibodies 38 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 23 Antibodies 23 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Western Blotting (WB) 106 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 4 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope N-Term
(58), (44), (41), (39), (27), (20), (20), (15), (14), (13), (9), (9), (8), (8), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(454), (216), (155), (27), (23), (22), (19), (19), (16), (13), (7), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(317), (151), (21), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(23), (23), (21), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(384), (184), (151), (96), (66), (57), (50), (33), (30), (18), (9), (6), (4), (3), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN359630) Images
Specificity This antibody reacts to ERK1.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERK1.
Isotype Ig

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN359630) Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2). ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595, 9606
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN359630) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN359630) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAPK3 Antibody (ABIN359630)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Background publications

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002

Charest, Mordret, Harder, Jirik, Pelech: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993

Owaki, Makar, Boulton, Cobb, Geppert: "Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs." in: Biochemical and biophysical research communications, Vol. 182, Issue 3, pp. 1416-22, 1992

Gonzalez, Raden, Rigby, Davis: "Heterogeneous expression of four MAP kinase isoforms in human tissues." in: FEBS letters, Vol. 304, Issue 2-3, pp. 170-8, 1992

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN359630) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (N-Term) (ABIN359630) (TOP)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primar...
Western Blotting (WB) image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (N-Term) (ABIN359630) Western blot analysis of ERK1 (arrow) using rabbit ERK1 N-term Pab.293 cell lysates (...