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anti-Mouse (Murine) MAPK3 antibody for Immunohistochemistry (Frozen Sections)

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Reactivity
Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis
454
216
155
26
22
21
18
18
16
13
7
6
4
4
4
4
3
3
2
2
2
1
1
Host
Rabbit
317
151
21
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
23
23
21
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
384
184
151
95
66
57
50
33
30
18
10
5
4
3
2
2
2
1
1
Supplier
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Catalog No. ABIN264862
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088293775 ABIN372623 IHC (fro) IF WB Rabbit IgG pThr202, Tyr204 Log in to see Polyclonal 4
0.00088293775 ABIN126829 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4
0.00088293775 ABIN126831 EIA IHC (fro) IF IP WB Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4
0.00088293775 ABIN184401 IHC (fro) IP WB Rabbit Log in to see Polyclonal

Top referenced anti-MAPK3 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-MAPK3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Fruit Fly (Drosophila melanogaster) Sheep (Ovine) Xenopus laevis
Western Blotting (WB) 351 Antibodies 164 Antibodies 106 Antibodies 23 Antibodies 22 Antibodies 19 Antibodies 19 Antibodies 22 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 38 Antibodies 14 Antibodies 22 Antibodies 1 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies 29 Antibodies 28 Antibodies 1 Antibodies 3 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Reactivity Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis
(454), (216), (155), (26), (22), (21), (18), (18), (16), (13), (7), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(317), (151), (21), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(23), (23), (21), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(384), (184), (151), (95), (66), (57), (50), (33), (30), (18), (10), (5), (4), (3), (2), (2), (2), (1), (1)
Pubmed 6 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN264862) Images
Purification Serum
Immunogen A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH
Isotype IgG

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN264862) Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).Synonyms: ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
Gene ID 50689
NCBI Accession NP_059043
UniProt P21708
Research Area Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN264862) Images back to top
Application Notes Western blot: 1: 5000 (ECL). Immunohistochemistry on frozen sections: 1: 50. Flow cytometry: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN264862) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

References for anti-MAPK3 Antibody (ABIN264862)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Background publications

Bhat, Gomis, Potter, Tikoo: "Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages." in: Molecular immunology, Vol. 47, Issue 6, pp. 1337-46, 2010

Aldinger, Qiu: "New mouse genetic model duplicates human 15q11-13 autistic phenotypes, or does it?" in: Disease models & mechanisms, Vol. 3, Issue 1-2, pp. 3-4, 2010

Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006

Zhuang, Schnellmann: "A death-promoting role for extracellular signal-regulated kinase." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 3, pp. 991-7, 2006

Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." in: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005

Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." in: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN264862) back to top
Supplier Images
 image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (ABIN264862) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
 image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (ABIN264862) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Image 2)