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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Cow (Bovine)||Fruit Fly (Drosophila melanogaster)||Sheep (Ovine)||Xenopus laevis|
|Western Blotting (WB)||284 Antibodies||123 Antibodies||103 Antibodies||23 Antibodies||22 Antibodies||19 Antibodies||19 Antibodies||22 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies||14 Antibodies||22 Antibodies||1 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||56 Antibodies||24 Antibodies||23 Antibodies||1 Antibodies||1 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis Alternatives|
|Conjugate||This MAPK3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
|9 references available|
|Supplier||Log in to see|
Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN361833) Images
|Specificity||Detects ~44kda (ERK1) and ~42 kDa (ERK2).|
|Immunogen||A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.|
Target Details MAPK3Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN361833) Images back to top
|Alternative Name||ERK1 (MAPK3 Antibody Abstract)|
The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Cellular Localization: Cytoplasm | Nucleus
|Research Area||Stem Cells, Alzheimer's Disease, Signaling, Inflammation|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN361833) Images back to top
|Application Notes||Recommended Dilution: WB (1:5000), IHC (1:100), ICC/IF (1:100), FCM (1:100), optimal dilutions for assays should be determined by the user.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN361833) Images back to top
References for anti-MAPK3 Antibody (ABIN361833)Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
|Product cited in:||
Revuelta-López, Cal, Herraiz-Martínez et al.: "Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes." in: Journal of molecular and cellular cardiology, Vol. 85, pp. 25-36, 2015 (PubMed).
Thon, Hosoi, Yoshii et al.: "Leptin induced GRP78 expression through the PI3K-mTOR pathway in neuronal cells." in: Scientific reports, Vol. 4, pp. 7096, 2014 (PubMed).
Enikanolaiye, Larivière, Troy et al.: "Involucrin-claudin-6 tail deletion mutant (CDelta206) transgenic mice: a model of delayed epidermal permeability barrier formation and repair." in: Disease models & mechanisms, Vol. 3, Issue 3-4, pp. 167-80, 2010 (PubMed).
Bhat, Gomis, Potter et al.: "Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages." in: Molecular immunology, Vol. 47, Issue 6, pp. 1337-46, 2010 (PubMed).
Chuderland, Marmor, Shainskaya et al.: "Calcium-mediated interactions regulate the subcellular localization of extracellular signal-regulated kinases." in: The Journal of biological chemistry, Vol. 283, Issue 17, pp. 11176-88, 2008 (PubMed).
Zhuang, Schnellmann: "A death-promoting role for extracellular signal-regulated kinase." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 3, pp. 991-7, 2006 (PubMed).
Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006 (PubMed).
Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." in: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005 (PubMed).
Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." in: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991 (PubMed).
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