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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||158 Antibodies||20 Antibodies||76 Antibodies||59 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||14 Antibodies||7 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies||5 Antibodies||9 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||56 Antibodies||26 Antibodies||17 Antibodies||5 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 9 (MAPK9) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPK9 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-MAPK9 AntibodyTarget Details MAPK9 Application Details Handling Images
|Specificity||This antibody specifically interacts with the N-terminus of SAPK1α/jnk2 kinases.|
|Characteristics||Synonyms: JNK-55, PRKM9, MAP kinase 9, Mitogen-activated protein kinase 9, Stress-activated proteinkinase JNK2, c-Jun N-terminal kinase 2|
|Purification||Subsequent thiophilic adsorbtion and Size Exclusion Chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic peptide conjugated to KLH.|
Target Details MAPK9Product Details anti-MAPK9 Antibody Application Details Handling Images back to top
|Alternative Name||MAPK9 / JNK2 (MAPK9 Antibody Abstract)|
|Background||Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1.Synonyms: JNK-55, MAP kinase 9, Mitogen-activated protein kinase 9, PRKM9, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2|
|Molecular Weight||54 kDa|
|Pathways||MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-MAPK9 Antibody Target Details MAPK9 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry on frozen sections. Positive Control: Cell lysate from untreated A431 cells. (See Protocol)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Positive Control Cell Lysate (A431 Untreated)|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK9 Antibody Target Details MAPK9 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mLPBS containing 0.09 % Sosium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: One year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-MAPK9 Antibody Target Details MAPK9 Application Details Handling back to top