You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PAK1 antibody for Enzyme Immunoassay

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • AW045634
  • pak
  • PAK-1
  • pak-1
  • Paka
  • pakalpha
  • PAKalpha
  • X-PAK1
  • XPak1
Epitope
Thr423
55
25
24
17
13
10
10
10
10
9
7
7
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
319
153
149
4
4
1
1
Host
Rabbit
289
17
15
1
Clonality
Polyclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
225
127
125
58
53
44
22
22
16
7
5
4
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN360317
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN358296 EIA Rabbit Ig pThr212 Log in to see Polyclonal 5
0.0008870071 ABIN358352 EIA Rabbit Ig pThr423 Log in to see Polyclonal 5
0.0008870071 ABIN272054 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008870071 ABIN782427 EIA IHC (p) WB Mouse IgG1 AA 191-280 Log in to see 1-00E-011
0.0008870071 ABIN117984 EIA Rabbit pThr423 Log in to see Polyclonal

Top referenced anti-PAK1 antibody for Enzyme Immunoassay

Similar anti-PAK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 22 Antibodies
ELISA 126 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunodiffusion (ID) 7 Antibodies
Immunofluorescence (IF) 60 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 125 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Western Blotting (WB) 229 Antibodies

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Epitope Thr423
(55), (25), (24), (17), (13), (10), (10), (10), (10), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(319), (153), (149), (4), (4), (1), (1)
Host Rabbit
(289), (17), (15), (1)
Clonality
Polyclonal
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(225), (127), (125), (58), (53), (44), (22), (22), (16), (7), (5), (4), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling References for anti-PAK1 Antibody (ABIN360317) Images
Specificity This antibody reacts to PAK1.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T423 of human PAK1.
Isotype Ig

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling References for anti-PAK1 Antibody (ABIN360317) Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK
Gene ID 5058, 9606
UniProt Q13153
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling References for anti-PAK1 Antibody (ABIN360317) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details References for anti-PAK1 Antibody (ABIN360317) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PAK1 Antibody (ABIN360317)

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling Images back to top
Background publications

Chen, Yin, Zhu, Yan, Ji, Chen, Cai, Zhang, Zong, Hu, Yuan, Shen, Gu: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003

Sells, Knaus, Bagrodia, Ambrose, Bokoch, Chernoff: "Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells." in: Current biology : CB, Vol. 7, Issue 3, pp. 202-10, 1997

Brown, Stowers, Baer, Trejo, Coughlin, Chant: "Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway." in: Current biology : CB, Vol. 6, Issue 5, pp. 598-605, 1997

Images

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling References for anti-PAK1 Antibody (ABIN360317) back to top
Supplier Images
 image for anti-PAK1 antibody (P21-Activated Kinase 1) (Thr423) (ABIN360317) anti-P21-Activated Kinase 1 (PAK1) (Thr423) antibody
 image for anti-PAK1 antibody (P21-Activated Kinase 1) (Thr423) (ABIN360317) anti-P21-Activated Kinase 1 (PAK1) (Thr423) antibody (Image 2)