anti-Human PAK1 antibody for Enzyme Immunoassay

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • X-PAK1
  • XPak1
  • pak
  • pak-1
  • pakalpha
  • PAKalpha
  • AW045634
  • PAK-1
  • Paka
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • Protein PAK-1
  • pak1
  • PAK1
  • Pak1
  • pak-1
Epitope
Thr423
63
33
21
15
12
12
11
10
9
9
6
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
346
187
178
8
8
6
5
4
4
3
2
1
1
Host
Rabbit
312
17
16
1
Clonality
Polyclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
248
125
118
76
55
52
23
20
12
7
5
5
4
1
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Catalog No. ABIN360317
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358296 EIA Rabbit Ig pThr212 Log in to see Polyclonal 5
1 ABIN358352 EIA Rabbit Ig pThr423 Log in to see Polyclonal 5
1 ABIN782427 EIA IHC (p) WB Mouse IgG1 AA 191-280 Log in to see 1-00E-011
1 ABIN272054 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN117984 EIA Rabbit pThr423 Log in to see Polyclonal

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Epitope Thr423
(63), (33), (21), (15), (12), (12), (11), (10), (9), (9), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(346), (187), (178), (8), (8), (6), (5), (4), (4), (3), (2), (1), (1)
Host Rabbit
(312), (17), (16), (1)
Clonality
Polyclonal
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(248), (125), (118), (76), (55), (52), (23), (20), (12), (7), (5), (5), (4), (1)
Pubmed 3 references available
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Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling References for anti-PAK1 antibody (ABIN360317) Images
Specificity This antibody reacts to PAK1.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T423 of human PAK1.
Isotype Ig

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling References for anti-PAK1 antibody (ABIN360317) Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK
Gene ID 5058, 9606
UniProt Q13153
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling References for anti-PAK1 antibody (ABIN360317) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details References for anti-PAK1 antibody (ABIN360317) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PAK1 antibody (ABIN360317)

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling Images back to top
Background publications

Chen, Yin, Zhu, Yan, Ji, Chen, Cai, Zhang, Zong, Hu, Yuan, Shen, Gu: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003

Sells, Knaus, Bagrodia, Ambrose, Bokoch, Chernoff: "Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells." in: Current biology : CB, Vol. 7, Issue 3, pp. 202-10, 1997

Brown, Stowers, Baer, Trejo, Coughlin, Chant: "Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway." in: Current biology : CB, Vol. 6, Issue 5, pp. 598-605, 1997

Images

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Supplier Images
 image for anti-P21-Activated Kinase 1 (PAK1) (Thr423) antibody (ABIN360317) anti-P21-Activated Kinase 1 (PAK1) (Thr423) antibody
 image for anti-P21-Activated Kinase 1 (PAK1) (Thr423) antibody (ABIN360317) anti-P21-Activated Kinase 1 (PAK1) (Thr423) antibody (Image 2)