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|Antigen||P21-Activated Kinase 1 (PAK1) Antibodies|
|Conjugate||This PAK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-PAK1 AntibodyTarget Details PAK1 Application Details Handling References for anti-PAK1 antibody (ABIN360317) Images
|Specificity||This antibody reacts to PAK1.|
|Purification||Protein A column, followed by peptide affinity purification|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T423 of human PAK1.|
Target Details PAK1Product Details anti-PAK1 Antibody Application Details Handling References for anti-PAK1 antibody (ABIN360317) Images back to top
|Alternative Name||PAK1 (PAK1 Antibody Abstract)|
|Background||PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK|
|Gene ID||5058, 9606|
|Research Area||Cancer, Apoptosis/Necrosis, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis|
Application DetailsProduct Details anti-PAK1 Antibody Target Details PAK1 Handling References for anti-PAK1 antibody (ABIN360317) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK1 Antibody Target Details PAK1 Application Details References for anti-PAK1 antibody (ABIN360317) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PAK1 antibody (ABIN360317)Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling Images back to top
Chen, Yin, Zhu, Yan, Ji, Chen, Cai, Zhang, Zong, Hu, Yuan, Shen, Gu: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003
Sells, Knaus, Bagrodia, Ambrose, Bokoch, Chernoff: "Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells." in: Current biology : CB, Vol. 7, Issue 3, pp. 202-10, 1997
Brown, Stowers, Baer, Trejo, Coughlin, Chant: "Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway." in: Current biology : CB, Vol. 6, Issue 5, pp. 598-605, 1997
ImagesProduct Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling References for anti-PAK1 antibody (ABIN360317) back to top