You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PAK1 antibody for Flow Cytometry

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • AW045634
  • pak
  • PAK-1
  • pak-1
  • Paka
  • pakalpha
  • PAKalpha
  • X-PAK1
  • XPak1
Epitope
N-Term
55
26
24
17
13
10
10
10
10
9
7
7
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
321
154
150
4
4
1
1
Host
Rabbit
290
17
16
1
Clonality
Monoclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
227
127
126
58
54
44
22
22
16
7
6
4
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN189638
Contact our Customer Service for availability and price in your country.

Similar anti-PAK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 34 Antibodies 34 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 3 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies 14 Antibodies
Immunofluorescence (IF) 39 Antibodies 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 70 Antibodies 71 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies 32 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 99 Antibodies

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Epitope N-Term
(55), (26), (24), (17), (13), (10), (10), (10), (10), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(321), (154), (150), (4), (4), (1), (1)
Host Rabbit
(290), (17), (16), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(227), (127), (126), (58), (54), (44), (22), (22), (16), (7), (6), (4), (1)
Supplier Log in to see

Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling Images
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues in the N-terminus of human PAK1 protein was used as immunogen.
Clone EP795Y
Isotype IgG

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background P21-activated kinase 1 (PAK1) is a member of a family of serine/threonine proteinkinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). ThePAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 andPAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility,apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5).PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5). Alternate Names: anti-Alpha PAK antibody, anti-Alpha PAK antibody, anti-p21 activated kinase 1 antibody,anti-p21 activated kinase1 antibody, anti-p21/Cdc42/Rac1 activated kinase 1 antibody,anti-p65 PAK antibody, anti-PAK 1 antibody, anti-PAK alpha antibody,anti-Serine/threonine protein kinase PAK 1 antibody, anti-Serine/threonine proteinkinase PAK1 antibody, anti-STE20 homolog (yeast) antibody.
Gene Symbol: PAK1
Gene ID 5058, 18479, 29431
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling Images back to top
Application Notes Recommended dilutions: Flow Cytometry 1:10, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:500
Protocol Protocol specific for PAK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PAK1 antibody (P21-Activated Kinase 1) (N-Term) (ABIN189638) Western blot analysis of anti- PAK1 (N-term) RabMAb (cat. # 110-57316), dilution 1:50...
Immunohistochemistry (IHC) image for anti-PAK1 antibody (P21-Activated Kinase 1) (N-Term) (ABIN189638) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-P...
Immunofluorescence (IF) image for anti-PAK1 antibody (P21-Activated Kinase 1) (N-Term) (ABIN189638) Immunofluorescent staining of HeLa cells using anti-PAK1 (N-term) RabMAb (cat. # 110-...